A5091179496- Bioinformatics and Genomic Networks
- Microbial Metabolic Engineering and Bioproduction
- Computational Drug Discovery Methods
- Hippo pathway signaling and YAP/TAZ
- Advanced Neuroimaging Techniques and Applications
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Neuroscience and Neural Engineering
- Spaceflight effects on biology
- Curcumin's Biomedical Applications
- Cell Image Analysis Techniques
- Ginkgo biloba and Cashew Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Nerve injury and regeneration
- Ubiquitin and proteasome pathways
- Epilepsy research and treatment
- 3D Printing in Biomedical Research
- Biotin and Related Studies
- Metabolism, Diabetes, and Cancer
- Nanoparticles: synthesis and applications
- Spinal Cord Injury Research
- Medicinal Plants and Neuroprotection
- Neurogenesis and neuroplasticity mechanisms
Istanbul Medipol University
2017-2025
Memorial Sloan Kettering Cancer Center
2023-2025
Türkisch-Deutsche Universität
2022
Abstract Systems-level assessments of protein-protein interaction (PPI) network dysfunctions are currently out-of-reach because approaches enabling proteome-wide identification, analysis, and modulation context-specific PPI changes in native (unengineered) cells tissues lacking. Herein, we take advantage chemical binders maladaptive scaffolding structures termed epichaperomes develop an epichaperome-based ‘omics platform, epichaperomics, to identify alterations disease. We provide multiple...
The intricate network of protein-chaperone interactions is crucial for maintaining cellular function. Recent discoveries have unveiled the existence specialized chaperone assemblies, known as epichaperomes, which serve scaffolding platforms that orchestrate reconfiguration protein-protein interaction networks, thereby enhancing adaptability and proliferation. This study explores structural regulatory aspects with a particular focus on role post-translational modifications (PTMs) in their...
The axolotl (Ambystoma mexicanum), a neotenic salamander with remarkable regenerative capabilities, serves as key model for studying nervous system regeneration. Despite its potential, the cellular and molecular mechanisms underlying this capacity remain poorly understood, partly due to lack of reliable in vitro models neural cells. In study, we developed novel protocol primary cultures adult telencephalon/pallium, enabling maintenance viable functionally active Using calcium imaging...
The ability of certain tetrapods, such as amphibians, to regenerate complex structures, organs or limbs, is well-established, though this capacity varies significantly across species, with humans exhibiting limited regenerative potential. Ependymoglia cells in the ventricular region brain are known exhibit proliferative properties during homeostasis and damage perform stem cell functions. This study investigated changes occurring neurons glia central nervous system following metamorphosis...
Alzheimer's disease (AD) progresses as a continuum, from preclinical stages to late-stage cognitive decline, yet the molecular mechanisms driving this progression remain poorly understood. Here, we provide systems-level map of protein-protein interaction (PPI) network dysfunction across AD spectrum and uncover epichaperomes-stable scaffolding platforms formed by chaperones co-factors-as central drivers process. Using over 100 human brain specimens, mouse models, neurons, show that...
Purpose: Curcumin, a polyphenol isolated from the rhizomes of turmeric, holds great potential as neuroprotective agent in addition to its anti-inflammatory and antioxidant characteristics. The poor bioavailability low stability curcumin are greatest barriers clinical use. This study aims investigate effect on axonal injury, by delivering lipophilic primary hippocampal neuron culture means lipid-based drug delivery system, named emulsomes. Methods: To neuroregeneration ex vivo, an injury...
Change in the energy metabolism of cancer cells, which display significant differences compared to normal is a rising phenomenon developing new therapeutic approaches against cancers. One metabolic enzymes, hexokinase-II (HK-II) involved glycolysis, and inhibiting HK-II activity may be potential target for therapy as most drugs clinical use act on DNA damage. Methyl jasmonate (MJ) one compounds blocking cells. In previous study, we showed that novel MJ analogs inhibit through VDAC detachment...
Trauma, vascular events, or neurodegenerative processes can lead to axonal injury and eventual transection (axotomy). Neurons survive axotomy, yet the underlying mechanisms are not fully understood. Excessive water entry into injured neurons poses a particular risk due swelling subsequent death. Using in vitro vivo neurotrauma model systems based on laser surgical nerve cut, we demonstrated that axotomy triggers actomyosin contraction coupled with calpain activity. As consequence, shrink...
Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) nanotechnology with antimicrobial properties. However, exposure to in daily life is issue for public health, still being updated. Aim was evaluate the effects AgNPs on development zebrafish embryos focusing Wnt pathway, proliferation, oxidant-antioxidant status, and apoptosis. The expressions ccnd1 gsk3β were determined by RT-PCR, whereas β-catenin proliferative...
Mesenchymal stem cells have the ability to transdifferentiate into neurons and therefore one of potential adult cell source for neuronal tissue regeneration applications understanding neurodevelopmental processes. In many studies on human mesenchymal (hMSC) derived neurons, success in differentiation was limited protein expressions which is not statisfactory terms activity. Established networks seen culture be investigated synaptic signal transmission develop a model further studying...
Neurodegenerative disorders, including Alzheimer’s disease (AD) and Parkinson’s (PD), represent debilitating conditions with complex, poorly understood pathologies. Epichaperomes, pathologic protein assemblies nucleated on key chaperones, have emerged as critical players in the molecular dysfunction underlying these disorders. In this study, we introduce synthesis characterization of clickable epichaperome probes, PU-TCO, positive control, PU-NTCO, negative control. Through comprehensive...
Regeneration of nerve tissue is a challenging issue in regenerative medicine. Especially, the peripheral defects related to accidents are one leading health problems. For large degeneration nerve, grafts used order obtain connection. These should be biodegradable prevent second surgical intervention. In make more effective engineering materials, nanotechnological improvements were used. addition electrically conductive and biocompatible metallic particles carbon structures has essential...
ABSTRACT Mesenchymal stem cells are one of the promising tissue specific cell source for neural regeneration applications. Previous studies on human mesenchymal (hMSC) derived neurons have been limited and not statisfactory in terms neuronal activity. In this study, we analysed functionality bone marrow hMSCs differentiated into protein expressing by a single step cytokine based induction protocol. Neurons from both primary hMSC line displayed spontaneous activity (≥75%) as demonstrated Ca...
ÖZETNanokorlar, bilinen en küçük ve fonksiyonel antikor fragmanlarıdır.Yapısında sadece iki ağır zincir bulunduran antikorların (heavy chain only antibodies; HcAb) antijen bağlama bölgesi, "nanokor" (nanobody) olarak adlandırılmıştır.Böylelikle bütün bir molekülü kadar olan zincirden oluşan (variable domain of the heavy HcAb; VHH) fragmanı tanımlanmıştır.Nanokorlar, boyutları sayesinde ulaşamadığı bölgelere dahi bağlanabilmektedir.Bunun yanında immün tepkimelere yol açmamaları görüntülemede...
Abstract Primary hyperparathyroidism is caused by solitary parathyroid adenomas (PTAs) in most cases (⁓85%), and it has been previously reported that PTAs are associated with cardiovascular disease (CVD) type-2 diabetes (T2D). To understand the molecular basis of PTAs, we have investigated genetic association amongst CVD T2D through an integrative network-based approach observed a remarkable resemblance. The current study proposed to compare PTAs-associated proteins overlapping determine...
Abstract The intricate protein-chaperone network is vital for cellular function. Recent discoveries have unveiled the existence of specialized chaperone complexes called epichaperomes, protein assemblies orchestrating reconfiguration protein-protein interaction networks, enhancing adaptability and proliferation. This study delves into structural regulatory aspects with a particular emphasis on significance post-translational modifications in shaping their formation A central finding this...
Abstract Trauma, vascular events, or neurodegenerative processes can lead to axonal injury and eventual transection (axotomy). Neurons survive axotomy, yet the underlying mechanisms are not fully understood. Excessive water entry into injured neurons poses a particular risk due swelling subsequent death. Using in vitro vivo neurotrauma model systems based on laser transection, we demonstrated that axotomy triggers actomyosin contraction coupled with calpain activity. As consequence, shrink...