A5079678635- Bacterial Genetics and Biotechnology
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Bacterial biofilms and quorum sensing
- ATP Synthase and ATPases Research
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
- Signaling Pathways in Disease
- Bacillus and Francisella bacterial research
- Lysosomal Storage Disorders Research
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Biosensors and Analytical Detection
- Machine Learning in Bioinformatics
- Neuroscience and Neuropharmacology Research
- Protein Structure and Dynamics
Yale University
2018-2024
Fundación Biofísica Bizkaia
2010-2018
Instituto Biofisika
2017-2018
University of the Basque Country
2008-2017
Consejo Superior de Investigaciones Científicas
2008-2014
The phospholipid cardiolipin (CL) has been proposed to play a role in selective mitochondrial autophagy, or mitophagy. CL externalization the outer membrane would act as signal for human Atg8 ortholog subfamily, MAP1LC3 (LC3). latter mediate both recognition and autophagosome formation, ultimately leading removal of damaged mitochondria. We have applied quantitative biophysical techniques study interaction with various orthologs, namely LC3B, GABARAPL2 GABARAP. found that LC3B interacts...
Many cellular activities, such as cell migration, division, phagocytosis, and exo-endocytosis, generate are regulated by membrane tension gradients. Membrane gradients drive flows, but there is controversy over how rapidly plasma flow can relax Here, we show that propagate or slowly, spanning orders of magnitude in speed, depending on the type. In a neuronal terminal specialized for rapid synaptic vesicle turnover, equilibrates within seconds. By contrast, does not neuroendocrine adrenal...
BAK is a key effector of mitochondrial outer membrane permeabilization (MOMP) whose molecular mechanism action remains to be fully dissected in intact cells, mainly due the inherent complexity intracellular apoptotic machinery. Here we show that core features BAK-driven MOMP pathway can reproduced highly simplified vitro system consisting recombinant human lacking carboxyl-terminal 21 residues (BAKΔC) and tBID combination with liposomes bearing an appropriate lipid environment. Using this...
Abstract Specific protein-lipid interactions lead to a gradual recruitment of AuTophaGy-related (ATG) proteins the nascent membrane during autophagosome (AP) formation. ATG3, key protein in movement LC3 towards isolation membrane, has been proposed facilitate LC3/GABARAP lipidation highly curved membranes. In this work we have performed biophysical study human ATG3 interaction with membranes containing phosphatidylethanolamine, phosphatidylcholine and anionic phospholipids. We found that...
Synaptotagmin-1 (Syt1) is a vesicular calcium sensor required for synchronous neurotransmitter release, composed of single-pass transmembrane domain linked to two C2 domains (C2A and C2B) that bind calcium, acidic lipids, SNARE proteins drive fusion the synaptic vesicle with plasma membrane. Despite its essential role, how Syt1 couples entry release poorly understood. Calcium binding C2B critical additionally binds complex. The C2A also function, but it not clear why. Here, we asked what...
BIM and tBID are two BCL-2 homology 3 (BH3)-only proteins with a particularly strong capacity to trigger BAX-driven mitochondrial outer membrane permeabilization, crucial event in mammalian apoptosis. However, the means whereby fulfill this task is controversial. Here, we used reconstituted liposomal system bearing physiological relevance explore systematically how BAX-permeabilizing function influenced by interactions of BIM/BID-derived BH3 motifs multidomain family members lipids. We found...
Little is known about mechanisms of membrane fission in bacteria despite their requirement for cytokinesis. The only dedicated machinery bacteria, protein B (FisB), expressed during sporulation Bacillus subtilis and required to release the developing spore into mother cell cytoplasm. Here, we characterized requirements FisB-mediated fission. FisB forms mobile clusters approximately 12 molecules that give way an immobile cluster at engulfment pole containing 40 proteins time Analysis mutants...
Increasing evidence indicates that the mitochondrial lipid membrane environment directly modulates BCL2 family protein function, but underlying mechanisms are still poorly understood. Here, we used minimalistic reconstituted systems to examine influence of lipids on MCL1 activity and conformation. Site-directed mutagenesis fluorescence spectroscopic analyses revealed homology region (MCL1ΔNΔC) inhibits permeabilization MOM-like membranes exclusively via canonical BH3-into-groove interactions...
Fluorescence microscopy has been one of the most discovery-rich methods in biology. In digital age, discipline is becoming increasingly quantitative. Virtually all biological laboratories have access to fluorescence microscopes, but abilities quantify biomolecule copy numbers are limited by complexity and sophistication associated with current quantification methods. Here, we present DNA-origami-based brightness standards for counting 5–300 copies proteins bacterial mammalian cells, tagged...
Membrane fission is an essential process in all domains of life. The underlying mechanisms remain poorly understood bacteria, partly because suitable assays are lacking. Here, we describe assay to detect membrane during endospore formation single Bacillus subtilis cells with a temporal resolution ∼1 min. Other cellular processes can be quantified and temporally aligned the event individual cells, revealing correlations causal relationships. For complete details on use execution this...
Over the past 15 years, hundreds of previously undiscovered bacterial small open reading frame (sORF)-encoded polypeptides (SEPs) fewer than fifty amino acids have been identified, and biological functions ascribed to an increasing number SEPs from intergenic regions RNAs. However, despite numbering in dozens
Synaptotagmin-1 (Syt1) is a major calcium sensor for rapid neurotransmitter release in neurons and hormone many neuroendocrine cells. It possesses two tandem cytosolic C2 domains that bind calcium, negatively charged phospholipids, the neuronal SNARE complex. Calcium binding to Syt1 triggers exocytosis, but how this occurs not well understood. has additional roles docking dense core vesicles (DCV) synaptic (SV) plasma membrane (PM) regulating fusion pore dynamics. Thus, perturbations could...
Abstract Fluorescence microscopy has been one of the most discovery-rich methods in biology. In digital age, discipline is becoming increasingly quantitative. Virtually all biological laboratories have access to fluorescence microscopes, but abilities quantify biomolecule copy numbers are limited by complexity and sophistication associated with current quantification methods. Here, we present DNA-origami-based brightness standards for counting 5–300 copies proteins mammalian bacterial cells,...