A5058368282- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- CRISPR and Genetic Engineering
- EEG and Brain-Computer Interfaces
Boston University
2020-2021
Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane
Fibronectin intrabodies generated with mRNA display (FingRs) are a recently developed tool for labeling excitatory or inhibitory synapses, the benefit of not altering endogenous synaptic protein expression levels transmission. Here, we viral vector FingR toolbox that allows multi-color, neuron-type-specific synapses in multiple brain regions. We screened various fluorophores, fusion configurations, and transcriptional control regulations adeno-associated virus (AAV) retrovirus designs....
Abstract Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold membrane dynamics from single neurons the mammalian brain. To perform high speed imaging, widefield microscopy remains an essential tool for recording activity many simultaneously over a large anatomical area. However, lack sectioning makes more prone to background signal contamination, thus far using fully limited simultaneous sampling few cells restricted...
Recent improvements in genetically encoded voltage indicators have enabled optical imaging of action potentials and subthreshold membrane from single neurons the mammalian brain. However, most current techniques can only simultaneously sample a few cell, limited either by strong background or small field-of-view. We show that, both theoretically experimentally, targeted illumination with widefield microscopy significantly improve performance improving signal contrast reducing...