A5050657570- T-cell and B-cell Immunology
- Optical Imaging and Spectroscopy Techniques
- Immune Cell Function and Interaction
- Non-Invasive Vital Sign Monitoring
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- Allergic Rhinitis and Sensitization
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Systemic Lupus Erythematosus Research
- Autoimmune and Inflammatory Disorders Research
- Asthma and respiratory diseases
- Mosquito-borne diseases and control
- Immune cells in cancer
- Photoacoustic and Ultrasonic Imaging
- Single-cell and spatial transcriptomics
- Hemodynamic Monitoring and Therapy
- Laser Applications in Dentistry and Medicine
- Glycosylation and Glycoproteins Research
- Diabetic Foot Ulcer Assessment and Management
- Renal Diseases and Glomerulopathies
- Climate Change and Health Impacts
- Genetic Syndromes and Imprinting
- Cardiac Ischemia and Reperfusion
Georgia Institute of Technology
2018-2024
University of Electronic Science and Technology of China
2014-2023
Immunitas Therapeutics (United States)
2023
Virginia Tech
2019
Sichuan University
2018
Human bone marrow (BM) plasma cells are heterogeneous, ranging from newly arrived antibody-secreting (ASCs) to long-lived (LLPCs). We provide single-cell transcriptional resolution of 17,347 BM ASCs five healthy adults. Fifteen clusters identified minted (cluster 1) expressing MKI67 and high major histocompatibility complex (MHC) class II that progress late 5–8 through intermediate 2–4. Additional ASC include the following: immunoglobulin (Ig) M predominant (likely extra-follicular origin),...
Abstract Intervertebral disc (IVD) disease (IDD) is a complex, multifactorial disease. While various aspects of IDD progression have been reported, the underlying molecular pathways and transcriptional networks that govern maintenance healthy nucleus pulposus (NP) annulus fibrosus (AF) not fully elucidated. We defined transcriptome map human IVD by performing single-cell RNA-sequencing (scRNA-seq) in primary AF NP cells isolated from non-degenerated lumbar disc. Our systematic comprehensive...
Immunoglobulin (Ig) E is central to the pathogenesis of allergic conditions, including fungal rhinosinusitis. However, little known about IgE antibody secreting cells (ASCs). We performed single-cell RNA sequencing from cluster differentiation (CD)19+ and CD19− ASCs nasal polyps patients with rhinosinusitis (n = 3). Nasal were highly enriched in CD19+ ASCs. Class-switched IgG IgA dominant (95.8%), whereas rare (2%) found only compartment. Through Ig gene repertoire analysis, shared clones...
Clinical monitoring of shock mainly depends on blood-oxygen-indices obtained from invasive blood sample tests. The central internal jugular vein oxygenation level (ScvO2) has been considered as a gold standard indicator for prediction. We developed noninvasive spatially-resolved near-infrared spectroscopy (SR-NIRS) to measure tissue oxygen saturation (StO2) surrounding the region taking ScvO2 test in 25 patients with shock. StO2 values were found be highly correlated (r = 0.84, p < 0.001)...
Antibody secreting cells (ASCs) circulate after vaccination and infection migrate to the BM where a subset known as long-lived plasma (LLPCs) persists secrete antibodies for lifetime. The mechanisms by which circulating ASCs become LLPCs are not well elucidated. Here, we show that human blood have distinct morphology, transcriptomes, epigenetics compared with LLPCs. Compared ASCs, decreased nucleus/cytoplasm ratio but increased endoplasmic reticulum numbers of mitochondria. up-regulate...
Modeling Light propagation within human head to deduce spatial sensitivity distribution (SSD) is important for Near-infrared spectroscopy (NIRS)/imaging (NIRI) and diffuse correlation tomography. Lots of models have been used on this issue, including layered model, artificial simplified MRI slices described visible model. Hereinto, Chinese (VCH) model considered be a most faithful presentation anatomical structure, has highlighted employed in modeling light propagation. However, it not...
Juvenile Idiopathic Arthritis (JIA) is an autoimmune disease with a heterogenous clinical presentation and unpredictable response to available therapies. This personalized transcriptomics study sought proof-of-concept for single-cell RNA sequencing characterize patient-specific immune profiles. Whole blood samples from six untreated children, newly diagnosed JIA, two healthy controls were cultured 24 h or without ex vivo TNF stimulation subjected scRNAseq examine cellular populations...
Alcohol use prior to and during pregnancy remains a significant societal problem can lead developmental fetal abnormalities including compromised myocardia function increased risk for heart disease later in life. Alcohol-induced cardiac toxicity has traditionally been studied animal-based models. These models have limitations due physiological differences from human cardiomyocytes (CMs) are also not suitable high-throughput screening. We hypothesized that human-induced pluripotent stem...
Summary Spatial transcriptomics technologies enable the spatially resolved measurement of gene expression within a tissue specimen. With these technologies, researchers can investigate how cells organize into cellular niches which are defined as distinct regions in comprising specific composition cell types or phenotypes. While general-purpose software tools for exploratory analysis spatial data exist, there is need that specialize organization niches. This further enhance downstream...
Low level light therapy (LLLT) has been clinically utilized for many indications in medicine requiring protection from cell/tissue death, stimulation of healing and repair injuries, pain reduction, swelling inflammation. Presently, use LLLT to treat stroke, traumatic brain injury, cognitive dysfunction is attracting growing interest. Near-infrared can penetrate into the tissue, allowing noninvasive treatment be carried out with few treatment-related adverse events. Optimization effect...
To evaluate whether recovery from complicated malaria follows a common trajectory in terms of immunological mechanism or, rather, is highly individualized for each patient, we performed longitudinal gene expression profiling whole blood. RNA sequencing (RNAseq) was on blood samples obtained eight patients four consecutive days between hospital admission and discharge. Six were infected with Plasmodium falciparum, two vivax; one patient pregnant woman P. who hospitalized several weeks. The...
Antibody secreting cells (ASC) circulate after vaccination and migrate to the bone marrow (BM) where a subset known as long-lived plasma (LLPC) persist secrete antibodies for lifetime. The mechanisms of how circulating ASC become LLPC are not well elucidated. Here, we show that human blood ASCs have distinct morphology, transcriptomes, epigenetics compared BM LLPC. acquire transcriptional epigenetic changes in apoptosis pathway support their survival. Upregulation pro-survival gene...
Abstract Background: Intra-tumoral CD4+ and CD8+ T cells expressing inhibitory receptor CD161 have an effector memory phenotype with high cytotoxic potential. inhibits NK cell function in the presence of its ligand, CLEC2D. This suppression can be recovered by treatment IMT-009, a first class anti-CD161 antibody. Single transcriptomic analysis anti-PD1 refractory microsatellite stable colorectal tumors identified CD161+ PD1+ exhausted CD8 suggesting that may provide benefit these both as...
Human bone marrow (BM) plasma cells are heterogeneous, ranging from newly arrived antibody-secreting (ASC) to long-lived (LLPC). We provide single cell transcriptional resolution of 17,347 BM ASC 5 healthy adults. Fifteen clusters were identified minted (cluster 1) expressing MKI67 and high MHC Class II that progressed late 5-8 through intermediate 2-4. Additional included early IgM-predominant likely extra-follicular origin; IFN-responsive; mitochondrial activity ASC. Late ASCs...
Abstract Background Juvenile Idiopathic Arthritis (JIA) is an autoimmune disease with a heterogenous clinical presentation and unpredictable response to available therapies. This personalized transcriptomics study sought proof-of-concept for single-cell RNA sequencing characterize patient-specific immune profiles. Methods Whole blood samples from six untreated children, newly diagnosed JIA, two healthy controls were cultured 24hrs or without ex vivo TNF stimulation subjected scRNAseq examine...
Background: Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) represent a viable cell source for clinical application and other purposes, such as disease modeling drug discovery cardiovascular diseases. However, their immature phenotype limits utility these applications. It is widely known that the transition from fetal to adult heart during development accompanied by an alteration in energy metabolism glycolysis fatty acid oxidation (FAO), respectively. Using this metabolic...
Objective To study the feasibility of non-invasive spatially-resolved near-infrared spectroscopy (SR-NIRS) in clinical bedside monitoring shock. Methods The central venous blood samples 25 patients with shock were collected and internal jugular vein oxygenation (ScvO2) level was measured. The self-developed SR-NIRS device used to measure tissue oxygen saturation (StO2) surrounding region vein. In addition, artery (SaO2) partial pressure (PO2) also measured using conventional methods....
Abstract Allergic fungal rhinosinusitis (AFRS) is a subset of chronic with nasal polyposis (CRSwNP). AFRS primarily associated type 2 inflammatory profile including eosinophilic mucosal infiltration and high serum titers IgE. Patients have increased frequencies IgE ASCs within NP structures, although it unclear if these cells originate from germinal center (GC) reaction or undergo local extrafollicular differentiation class switch recombination (CSR). To better understand the origin cells,...