A5008311706- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Cervical and Thoracic Myelopathy
- Musculoskeletal pain and rehabilitation
- Scoliosis diagnosis and treatment
- Medical Imaging and Analysis
- Pelvic and Acetabular Injuries
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Bone Metabolism and Diseases
- Heterotopic Ossification and Related Conditions
- Connective tissue disorders research
- Spinal Hematomas and Complications
- Radiation Dose and Imaging
- Wnt/β-catenin signaling in development and cancer
- Osteoarthritis Treatment and Mechanisms
- Anesthesia and Pain Management
- TGF-β signaling in diseases
- Orthopedic Surgery and Rehabilitation
- Bone fractures and treatments
- Nerve Injury and Rehabilitation
- Peripheral Nerve Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Health Systems, Economic Evaluations, Quality of Life
Emory University
2016-2024
Atlanta VA Medical Center
2019-2024
New York University
2024
Veterans Health Administration
2020-2023
AO Foundation
2021
University of Pennsylvania
2021
Tokai University
2021
Medical Education Institute
2016
Rush University Medical Center
2016
Johns Hopkins Hospital
2016
Abstract Background Rats are a widely accepted preclinical model for evaluating intervertebral disc (IVD) degeneration and regeneration. IVD morphology is commonly assessed using histology, which forms the foundation quantifying state of degeneration. severity evaluated different grading systems that focus on distinct degenerative features. A standard system would facilitate more accurate comparison across laboratories robust comparisons models interventions. Aims This study aimed to develop...
Abstract Background In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes much‐needed progress field. Here, an international group spine scientists collaborated standardize extraction expansion techniques for NP reduce variability, improve comparability between labs utilization funding resources. Methods The most applied methods extraction,...
Abstract Intervertebral disc (IVD) disease (IDD) is a complex, multifactorial disease. While various aspects of IDD progression have been reported, the underlying molecular pathways and transcriptional networks that govern maintenance healthy nucleus pulposus (NP) annulus fibrosus (AF) not fully elucidated. We defined transcriptome map human IVD by performing single-cell RNA-sequencing (scRNA-seq) in primary AF NP cells isolated from non-degenerated lumbar disc. Our systematic comprehensive...
The chronic stinger syndrome is a distinct entity from acute stingers and has been shown to have its own pathophysiology that, unlike stingers, may reflect long-standing geometrical changes of the subaxial spinal canal irritation/degeneration exiting nerve root complex. There no method available, however, accurately predict these symptoms in athletes. mean cervical space available for cord (MSCSAC) novel alternative Torg ratio predicting neurological caused by spondylosis elite It goal this...
Degeneration of the intervertebral disc (IVD) results in deterioration spinal motion segment and can lead to debilitating back pain. Given established mitotic anti-apoptotic effects recombinant human platelet-derived growth factor-BB (rhPDGF-BB) a variety cell types we postulated that rhPDGF-BB might delay degeneration through inhibition apoptosis. To address this hypothesis, treated IVD cells isolated from five independent patients with monolayer 3D pellet cultures. The potential,...
The clinical healing environment after a posterior spinal arthrodesis surgery is one of the most clinically challenging bone-healing environments across all orthopedic interventions due to absence contained space and need form de novo bone. Our group has previously reported that sclerostin in expressed locally at high levels throughout developing fusion. However, role controlling bone fusion remains be established.We computationally identified two FDA-approved drugs, as well single novel...
Abstract Intervertebral disc degeneration (IDD) is a public health dilemma as it associated with low back and neck pain, frequent reason for patients to visit the physician. During IDD, nucleus pulposus (NP), central compartment of intervertebral (IVD) undergo degeneration. Stem cells have been adopted promising biological source regenerate IVD restore its function. Here, we describe simple, two‐step differentiation strategy using cocktail four factors (LDN, AGN, FGF, CHIR) efficient...
Abstract Background The rabbit lumbar spine is a commonly utilized model for studying intervertebral disc degeneration and the pre‐clinical evaluation of regenerative therapies. Histopathology foundation which alterations to morphology cellularity with degeneration, or following repair treatment are assessed. Despite this, no standardized histology grading scale has yet been established field any frequently animal models. Aims purpose this study was establish new scoring system assess...
Osteoinductive bone morphogenetic proteins (BMPs), including BMP-2, have a unique capability of mediating formation both in orthotopic and ectopic locations. Immunosuppresive macrolides been shown to potentiate BMP-2 activity through FKBP12, but these yet translate effective osteoinductive therapies. Herein, we show the osteogenic FK506 as stand-alone agent direct comparison vitro vivo. was capable producing alkaline phosphatase induction C2C12 cells comparable that seen with rhBMP-2....
Study Design Systematic Review and Meta-Analysis. Objectives To compare complication incidence in patients with or without the use of recombinant human Bone Morphogenic Protein-2 (BMP2) undergoing anterior cervical discectomy fusion (ACDF) for degenerative conditions. Methods A systematic search eight online databases was conducted using PRISMA guidelines. Inclusion criteria included English language studies a minimum 10 adult instrumented ACDF surgery spinal condition which BMP2 used all...
Intervertebral disc (IVD) degeneration (IDD) is a multifactorial physiological process which often associated with lower back pain. Previous studies have identified some molecular markers degeneration, despite their significant contributions, provided limited insight into the etiology of IDD. In this study, we utilized network medicine approach to uncover potential mediators Our systematic analyses IDD 284 genes included functional annotation clustering, interaction networks, cluster...
To determine the risk factors associated with radiographic changes and clinical outcomes following 3-level anterior cervical discectomy fusion (ACDF) using rigidplate constructs cortico-cancellous allograft. ACDF has demonstrated efficacy for treatment of multilevel degenerative conditions, but current data exists in small heterogeneous forms.A retrospective review included 98 patients primary surgery at one institution from 2008 to 2013 minimum 1-year follow-up. Cervical sagittal vertical...
Intervertebral disc degeneration is the main contributor to low back pain, now leading cause of disability worldwide. Gene transfer, either in a therapeutic attempt or basic research understand mechanisms degeneration, fascinating and promising tool manipulate complex physiology disc. Viral vectors based on adeno-associated virus (AAV) have emerged as powerful transgene delivery vehicles yet systematic investigation into their respective tropism, transduction efficiency, relative toxicity...
Abstract Bone grafting procedures are commonly used for the repair, regeneration, and fusion of bones in a wide range orthopaedic surgeries, including large bone defects spine procedures. Autografts clinical gold standard, though recombinant human morphogenetic proteins (rhBMPs) often used, particularly difficult situations. However, treatment with rhBMPs can have off-target effects significantly increase surgical costs, adding to patients’ already high economic mental burden. Recent studies...
Abstract The bone healing environment in the posterolateral spine following arthrodesis surgery is one of most challenging all orthopedics and our understanding molecular signaling pathways mediating osteogenesis during spinal fusion limited. In this study, spatial temporal expression pattern Wnt factors inhibitors was assessed for first time. Bilateral with autologous iliac crest graft performed on 21 New Zealand White rabbits. At 1‐, 2‐, 3‐, 4‐, 6‐weeks, sclerostin a variety canonical...
Massel, Dustin H. BS; Puvanesarajah, Varun MD; Mayo, Benjamin C. BA; Hassanzadeh, Hamid Presciutti, Steven M. Phillips, Frank MD Author Information