A5002522621- Spine and Intervertebral Disc Pathology
- Veterinary Orthopedics and Neurology
- Cervical and Thoracic Myelopathy
- Musculoskeletal pain and rehabilitation
- TGF-β signaling in diseases
- Genetics and Neurodevelopmental Disorders
- Retinal Development and Disorders
- Orthopaedic implants and arthroplasty
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Tendon Structure and Treatment
- Neurogenetic and Muscular Disorders Research
- Medical Imaging and Analysis
- Pancreatic function and diabetes
Utrecht University
2017-2023
Abstract Background In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes much‐needed progress field. Here, an international group spine scientists collaborated standardize extraction expansion techniques for NP reduce variability, improve comparability between labs utilization funding resources. Methods The most applied methods extraction,...
Abstract Background Lineage‐tracing experiments have established that the central region of mature intervertebral disc, nucleus pulposus (NP), develops from embryonic structure called “the notochord”. However, changes in cells derived notochord which form NP (i.e., notochordal [NCs]), terms their phenotype and functional identity early developmental stages to skeletal maturation are less understood. These key issues require further investigation better comprehend role NCs homeostasis...
Degeneration of the intervertebral disc (IVD) is a frequent cause for back pain in humans and dogs. Link-N stabilizes proteoglycan aggregates cartilaginous tissues exerts growth factor-like effects. The human variant facilitates IVD regeneration several species vitro by inducing Smad1 signaling, but it not clear whether this specific. Dogs with disease could possibly benefit from treatment, has been tested on canine cells. If appears to be effective canines, would facilitate translation into...
Repopulating the degenerated intervertebral disc (IVD) with tissue-specific nucleus pulposus cells (NPCs) has already been shown to promote regeneration in various species. Yet applicability of NPCs as cell-based therapy hampered by low cell numbers that can be extracted from donor IVDs and their potentially limited regenerative capacity due phenotype. To optimize expansion conditions, we investigated effects increasing culture medium osmolarity during on phenotype dog ability produce a...