A5085759890- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Orthopaedic implants and arthroplasty
- Osteoarthritis Treatment and Mechanisms
- Shoulder Injury and Treatment
- Bone Tissue Engineering Materials
- Cervical and Thoracic Myelopathy
- 3D Printing in Biomedical Research
- Legal and Policy Issues
- Veterinary Orthopedics and Neurology
- Spinal Hematomas and Complications
- Anesthesia and Pain Management
- Silk-based biomaterials and applications
- Pain Management and Opioid Use
- Myofascial pain diagnosis and treatment
- Tendon Structure and Treatment
Trinity College Dublin
2021-2024
University of Pennsylvania
2022
AO Foundation
2022
Tokai University
2022
Modular biofabrication strategies using microtissues or organoids as biological building blocks have great potential for engineering replacement tissues and organs at scale. Here we describe the development of a strategy to engineer osteochondral by spatially localising phenotypically distinct cartilage within an instructive 3D printed polymer framework. We first demonstrate that immature can spontaneously fuse form homogeneous macrotissues, combining less cellular results in superior fusion...
Abstract Background In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes much‐needed progress field. Here, an international group spine scientists collaborated standardize extraction expansion techniques for NP reduce variability, improve comparability between labs utilization funding resources. Methods The most applied methods extraction,...
A significant hurdle for potential cell-based therapies is the subsequent survival and regenerative capacity of implanted cells. While many exciting developments have demonstrated promise preclinically, intervertebral disc (IVD) degeneration fail to translate equivalent clinical efficacy.This work aims ascertain relevance both a small large animal model by experimentally investigating comparing these models human from perspective anatomical scale their cellular metabolic potential.First,...
Abstract Background Despite exciting advances in regenerative medicine, cell‐based strategies for treating degenerative disc disease remain their infancy. To maximize the potential successful clinical translation, a more thorough understanding of vivo microenvironment is needed to better determine and predict how cell therapies will respond when administered vivo. Aims This work aims reflect on nutrient degenerating IVD through consolidating what has already been measured together with...
Ex vivo disc organ culture systems have become a valuable tool for the development and pre-clinical testing of potential intervertebral (IVD) regeneration strategies. Bovine caudal discs been widely selected due to their large availability comparability human IVDs in terms size biochemical composition. However, despite extensive use, it remains be elucidated whether nutrient microenvironment is comparable degeneration.This work aims create first experimentally validated silico model which...
Abstract During intervertebral disc (IVD) degeneration, microenvironmental challenges such as decreasing levels of glucose, oxygen, and pH play crucial roles in cell survival matrix turnover. Antacids, Mg(OH) 2 CaCO 3 , entrapped microcapsules are capable neutralizing acidic microenvironments a controlled fashion therefore may offer the potential to improve niche degenerated IVD enhance cell‐based regeneration strategies. The objectives this work were, first, develop characterize antacid...
Abstract Background While growth factors have the potential to halt degeneration and decrease inflammation in animal models, literature investigating effect of dosage on human cells is lacking. Moreover, despite completion clinical trials using differentiation factor‐5 (GDF‐5), no results been publicly released. Aims The overall objective was quantitatively assess three clinically relevant concentrations GDF‐5 (0.25, 1, 2 mg) as a therapeutic for disc regeneration. Materials methods Firstly,...
Abstract Background It is well established that the unique biochemical microenvironment of intervertebral disc plays a predominant role in cell viability and biosynthesis. However, unless effect microenvironmental conditions primary to study objective, vitro culture parameters are critical for reproducibility both varied not routinely reported. Aims This work aims investigate local microenvironments commonly used configurations, highlighting physiological relevance, potential discrepancies,...
There has been significant interest in identifying alternative cell sources and growth factor stimulation to improve matrix synthesis for disc repair. Recent work identified nasoseptal chondrocytes (NC) as a possible source with matrix-forming abilities. While various factors such members of the TGFβ superfamily have explored enhance formation, no consensus exists optimum needed induce cells towards discogenic phenotype. This study assessed both nucleus pulposus (NP) NC microtissues...