A5074491988- Forensic Toxicology and Drug Analysis
- Alcohol Consumption and Health Effects
- Traffic control and management
- Autonomous Vehicle Technology and Safety
- Pharmaceutical studies and practices
- Metabolomics and Mass Spectrometry Studies
- Vehicular Ad Hoc Networks (VANETs)
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Metabolism and Genetic Disorders
- Microbial bioremediation and biosurfactants
- Analytical Chemistry and Chromatography
- Carcinogens and Genotoxicity Assessment
- Magnetism in coordination complexes
- Renal function and acid-base balance
- Analytical Methods in Pharmaceuticals
- Metal-Catalyzed Oxygenation Mechanisms
- Lanthanide and Transition Metal Complexes
- Poisoning and overdose treatments
- Pharmacogenetics and Drug Metabolism
- Pesticide Residue Analysis and Safety
- Science, Research, and Medicine
- Cannabis and Cannabinoid Research
- Effects and risks of endocrine disrupting chemicals
- Microbial Metabolic Engineering and Bioproduction
BMW (Germany)
2021-2025
Heidelberg University
1999-2023
BMW Group (Germany)
2020-2023
University Hospital Heidelberg
2008-2023
Roche (Switzerland)
2012-2022
Vereinigung Analytischer Kinder- und Jugendlichen-Psychotherapeuten
2020
Philipps University of Marburg
2013-2019
University of Stuttgart
2010-2019
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2016
South Westphalia University of Applied Sciences
2005
Ethyl glucuronide (ethyl β-d-6-glucosiduronic acid), a minor ethanol metabolite in serum or urine, was determined by gas chromatography-mass spectrometry. Prior to this, ethyl synthesized the reaction of acetobromo-glucosiduronic acid with ethanol. For determination glucuronide, samples were precipitated acetone, and urine specimens analyzed after evaporation dryness. The residues derivatized acetic anhydride. Capillary chromatography used find retention index value 1920 for triacetyl...
The kinetic profile of ethanol and ethyl glucuronide (EtG) in serum was investigated three subject groups: 1) Healthy, moderately drinking volunteers (daily intake less than 30 g ethanol) who ingested a single dose ethanol. In this group the maximum concentration (SEtGC) (SEC) did not exceed 3.7 mg/L 1.5 g/L respectively. EtG peaked 2 to 3.5 h later eliminated with terminal half-life 3 h. decreased slower ethanol--the metabolite could still be determined up 8 after complete elimination. 2)...
With increasing connectivity, more and advanced forms of cooperation among vehicles have become possible. Research has shown that vehicle-to-vehicle communication can improve road safety. However, recent research advances regarding complex interactions such as cooperative maneuver negotiation not yet gained much attention. Thus, in this survey, we analyze proposals for coordination protocols automated vehicles. We include the communication, computation, decision-making architectures commonly...
Polysorbates 20 and 80 are the most frequently used excipients in biotherapeutics, safety data for which have been well documented adults. The polysorbate content therapeutic formulations that administered to children, however, has less clearly regulated or defined with regard safety. In pediatric patients, excessive amounts of biotherapeutics linked hypersensitivity other toxicity-related effects. To determine safe levels polysorbates young we developed progressive factor (PPSF), an age-...
Ethyl glucuronide (EG) is a useful marker of alcohol consumption because its presence in urine can be detected up to five days. We investigated the impact diuresis on urinary excretion FG, minor ethanol metabolite. Seven healthy volunteers drank 250 mL wine (25 g ethanol) 15 min and, 240 later, ingested 1 L water within min. Urine was voided before drinking started and every 30–60 for 400–550 thereafter. Urinary ethyl (UEG), creatinine, were determined using liquid chromatography-tandem mass...
Journal Article Morphine and Glucuronides in Serum of Heroin Consumers Heroin-Related Deaths Determined by HPLC with Native Fluorescence Detection Get access Rolf Aderjan, Aderjan Institut für Rechtsmedizin im Klinikum, University Heidelberg, Vosstrasse 2, D-69115 Germany Search for other works this author on: Oxford Academic PubMed Google Scholar Sybille Hofmann, Hofmann Georg Schmitt, Schmitt Gisela Skopp Analytical Toxicology, Volume 19, Issue 3, May-June 1995, Pages 163–168,...
Rubber oxygenase A (RoxA) is one of only two known enzymes able to catalyze the oxidative cleavage latex for biodegradation. RoxA acts as a processive dioxygenase yield predominant product 12-oxo-4,8-dimethyl-trideca-4,8-diene-1-al (ODTD), tri-isoprene unit. Here we present structural analysis from Xanthomonas sp. strain 35Y at resolution 1.8 Å. The enzyme 75-kDa diheme c -type cytochrome with an unusually low degree secondary structure. Analysis heme group arrangement and peptide chain...
Phospholipidosis (PLD) is a lysosomal storage disorder induced by compounds, notably cationic amphiphilic drugs, which although reversible interferes with cellular phospholipids.The in silico method described utilizes the moment ΔΔG(AM) (kJ/mol) together basic pK(a) values to assign PLD inducing potential compound. The new model was accurate and sensitive (85% 82%, respectively) when compared other data sets. Therefore, parallel vitro assay for discontinued. reinforce our view that far more...
The rubber oxygenase (RoxA) of Xanthomonas sp. strain 35Y (RoxA(Xsp)) is so far the only known extracellular c-type diheme cytochrome that able to cleave poly(cis-1,4-isoprene). All other rubber-degrading bacteria described are Gram positive and employ a nonheme protein (latex-clearing [Lcp]) for postulated primary attack polyisoprene. Here, we identified RoxA orthologs in genomes Haliangium ochraceum, Myxococcus fulvus, Corallococcus coralloides, Chondromyces apiculatus. roxA H. ochraceum...
The biochemical properties of a new tungsten-containing aldehyde oxidoreductase from the mesophilic betaproteobacterium Aromatoleum aromaticum EbN1 (AOR Aa ) are presented in this study. enzyme was purified phenylalanine-grown cells an overexpressing mutant lacking gene for dehydrogenase normally involved anaerobic phenylalanine degradation. AOR catalyzes oxidation broad variety aldehydes to respective acids with either viologen dyes or NAD+ as electron acceptors. In contrast previously...
XCE, a new member of the endothelin-converting enzyme and neutral endopeptidase family, is preferentially expressed in specific areas central nervous system including spinal chord medulla. To elucidate importance function we disrupted its gene mouse embryonic stem cells by homologous recombination created mice deficient XCE. The resulting phenotype characterized neonatal lethality. All XCE −/− homozygous died respiratory failure shortly after birth, most cases their lungs were never...
Natural rubber [poly-(cis-1,4-isoprene)] is cleaved to 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (ODTD) by oxygenase A (RoxA) isolated from Xanthomonas sp. RoxA has two c-type haem centres that show distinct alpha-bands at 549 and 553 nm in the dithionite-reduced state. well-resolved midpoint potential (E(0)') of -65 mV was determined for one spectrophotometric titrations absence dioxygen with dithionite ferricyanide as reductant oxidant, respectively. The second not resolvable (E(0)' about...
Anaerobic phenylalanine metabolism in the denitrifying betaproteobacterium Aromatoleum aromaticum is initiated by conversion of to phenylacetate, which further metabolized via benzoyl-coenzyme A (CoA). The formation phenylacetate catalyzed transaminase, phenylpyruvate decarboxylase, and a phenylacetaldehyde-oxidizing enzyme. presence these enzymes was detected extracts cells grown with nitrate. We found that two distinct are involved oxidation phenylacetaldehyde an aldehyde:ferredoxin...
Application of hydrostatic pressure (≤ 10.5 kbar) on the two-dimensional spin transition compound [Fe(btr)2(NCS)2]·H2O (btr = 4,4'-bis-1,2,4-triazole) results in an unexpected stabilization HS state. On release pressure, state is found to be partially trapped. After thermal relaxation metastable obtained by LIESST effect (light-induced excited trapping), a pure LS contrast experiments. This different behavior supports structural phase as likely basis pressure-induced
Ethyl glucuronide is a highly specific metabolite of ethanol that formed by enzymatic conjugation with glucuronic acid. Because its suitability as marker excessive alcohol consumption in serum low blood-alcohol concentration and urine, especially after the breakdown ethanol, demand exists for simple fast analytical procedure, which rarely possible using mass spectrometric determination methods. For this reason, we developed an immunochemical screening procedure (ELISA) polyclonal antibodies...
RoxA is an extracellular c-type diheme cytochrome secreted by Xanthomonas sp. strain 35Y during growth on rubber. cleaves poly(cis-1,4-isoprene) to 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (ODTD). Analysis of the structure revealed that Phe317 located in close proximity (≈5 Å) N-terminal heme presumably represents active site. To find evidence whether important for catalysis, we changed it tyrosine, tryptophan, leucine, histidine, or alanine. All five muteins were expressed after...
In the past decade Göttingen minipig has gained increasing recognition as animal model in pharmaceutical and safety research because it recapitulates many aspects of human physiology metabolism. Genome-based comparison drug targets together with quantitative tissue expression analysis allows rational prediction pharmacology cross-reactivity drugs models thereby improving attrition which is an important challenge process development. Here we present a new chromosome level based version genome...