A5050124541- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Adipokines, Inflammation, and Metabolic Diseases
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- RNA modifications and cancer
- Cardiovascular Disease and Adiposity
- Eicosanoids and Hypertension Pharmacology
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Pancreatitis Pathology and Treatment
- Meat and Animal Product Quality
- Biochemical effects in animals
- Heme Oxygenase-1 and Carbon Monoxide
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Inflammasome and immune disorders
- Gene expression and cancer classification
- Ocular Oncology and Treatments
- Pancreatic and Hepatic Oncology Research
- Apelin-related biomedical research
- Plant Molecular Biology Research
- Amoebic Infections and Treatments
Duke-NUS Medical School
2015-2022
Xi'an Jiaotong University
2022
Wenzhou Medical University
2013-2020
Affiliated Eye Hospital of Wenzhou Medical College
2019
National University of Singapore
2014-2015
First Affiliated Hospital of Wenzhou Medical University
2014
Lund University
2014
National Institute of Biological Sciences, Beijing
2012
Brown adipose tissue (BAT) is specialized to burn lipids for heat generation as a natural defense against cold and obesity. Previous studies established microRNAs (miRNAs) essential regulators of brown adipocyte differentiation, but whether miRNAs are required the feature maintenance mature adipocytes remains unknown. To address this question, we ablated Dgcr8, key regulator miRNA biogenesis pathway, in well white adipocytes. Adipose tissue-specific Dgcr8 knockout mice displayed enlarged...
Abstract Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown and overexpression primary adipocyte culture enhances inhibits adipogenesis vitro, respectively. Fat-specific knockout significantly adipogenic gene program adipose tissues, accompanied by systemic glucose intolerance insulin resistance. also results depot-specific phenotypes: it can repress...
Enhancing brown fat activity and promoting white browning are attractive therapeutic strategies for treating obesity associated metabolic disorders. To provide a comprehensive picture of the gene regulatory network in these processes, we conducted series transcriptome studies by RNA sequencing (RNA-seq) quantified mRNA long noncoding (lncRNA) changes during (chronic cold exposure, beta-adrenergic agonist treatment, intense exercise) activation or inactivation (acute exposure...
Lipoxins (LXs) and their analogues are known to display potent anti-inflammatory actions. Previously, we reported that lipoxin A4 (LXA4) possessed powerful properties in acute pancreatitis rats it may ameliorate the concomitant lung injury by reducing cytokine generation inhibiting neutrophil activation. Considering vascular endothelium plays an important role during adherence, migration activation of leukocytes, present study was designed investigate effects LXA4 on inflammatory response...
Alternative splicing is involved in functional regulation of the mutagenic enzyme activation‐induced cytidine deaminase (AID). However, molecular basis for AID remains undefined. Using a mini‐gene‐based screen HeLa cells, we found that overexpression RNA binding motif protein 5 (RBM5, or LUCA‐15/H37) significantly promoted exon 4 skipping by suppressing intron 3. The inhibitive effect RBM5 on 3 required weak 3′‐splice site (ss). Indicative underlying mechanism, interfered with U2AF65 to...
Brown fat has gained widespread attention as a potential therapeutic target to treat obesity and associated metabolic disorders. Indeed, the anti-obesity of multiple targets stimulate both brown adipocyte differentiation recruitment have been verified in rodent models. However, their humans is unknown due lack human primary cell culture system. Likewise, well-characterized model limited discovery novel for activation fat. To address this current need, we aimed identify describe first system...
The detailed mechanism underlying adipocyte development and function remains to be fully understood. MicroRNAs are a class of non-coding regulators in physiological pathological conditions. Our recent study(11) using adipose-specific dgcr8 knockout mice demonstrates that microRNA biogenesis has depot-specific adipose plays an important role brown feature maintenance. Interestingly, parallel study from Kahn's group, Mori et al observed similar phenotypes Dicer mice. This also reveals the...
Abstract The execution of biological activities inside space‐limited cell nuclei requires sophisticated organization. Current studies on the 3D genome focus chromatin interactions and local structures, e.g., topologically associating domains (TADs). In this study, two global physical properties: DNA density distance to nuclear periphery (DisTP), are introduced a 2D matrix, D 2 plot, is constructed for mapping genetic epigenetic markers. Distinct patterns functional markers indicating its...
Uveal melanomas are the most common primary intraocular malignant tumors in adults, associated with a high metastasis rate and low 5-year survival rate. It is clinic urgency importance to identify prognostic factors for UVMs.55 aberrantly methylated sites of miRNAs 47 lncRNAs were observed between Alive < 2 years group > UVMs. Two classifiers generated. For 13- miRNAs-CpG-classifier, AUC 0.958, 0.848 0.824 at 1 year, 3 years, respectively. 9- lncRNAs-CpG-classifier, 0.943, 0.869 0.866...
Objective To investigate the effects of sleep deprivation on expressions Mir-132, mir-134 in different regions rat brain. Methods All male SD rats were divided into control group (normal group), (SD). The modified multiple platform method (MMPM) was used to establish model. level detected by real time PCR. Results Mir-132 significantly increased groups hippocampus compared with groups(51.87±8.13 vs 67.25±7.59)(P 0.05). Cortex mir-132 and 1.57±0.10, 1.48±0.11 respectively, it...