Abstract: Antioxidant activities of flavonoids were decreased in the order flavonols > flavanones flavones. Inhibitory intensities for hyaluronidase and collagenase reaction differed clearly according to flavonoid subclasses. Kaempferol, quercetin, myricetin, rutin inhibited specifically, while apigenin, luteolin, baicalin, baicalein flavones showed specific inhibition reaction. In addition, flavonoids, except baicalin catechin, potently LPS‐induced nitrite production a dose‐dependent...

Bardet Biedl syndrome (BBS) is a multisystem genetically heterogeneous ciliopathy that most commonly leads to obesity, photoreceptor degeneration, digit anomalies, genito-urinary abnormalities, as well cognitive impairment with autism, among other features. Sequencing of DNA sample from 17-year-old female affected BBS did not identify any mutation in the known genes. Whole-genome sequencing identified novel loss-of-function disease-causing homozygous (K102*) C8ORF37 , gene coding for cilia...

In this study, we investigated the mechanistic role of caspase cascade in extrinsic and intrinsic apoptosis induced by apigenin, which has been targeted as a candidate development noncytotoxic anticancer medicines. Treatment with apigenin (1-100 microM) significantly inhibited proliferation MDA-MB-453 human breast cancer cells dose- time-dependent manner IC(50) values 59.44 35.15 microM at 24 72 h, respectively. This inhibition resulted induction release cytochrome c exposed to its h IC(50)....

Daidzein and its glycoside form daidzin, are known to have potential health benefits metabolized O‑desmethylangolensin (O‑DMA) equol following consumption. In the current study, antioxidant activity cytotoxicity of O‑DMA, equol, daidzein daidzin was investigated their effects on HepG2 human hepatocelluar carcinoma cells were compared. For assays, lactose dehydrogenase (LDH) release 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide‑based cell viability, exposed various...

In this study, we investigated the antiproliferative activity of isoflavones daidzein and genistein in three breast cancer cell lines with different patterns estrogen receptor (ER) c‑erbB‑2 protein expression (ERα‑positive MCF‑7 cells, c‑erbB‑2‑positive SK‑BR‑3 cells ERα/c‑erbB‑2‑positive ZR‑75‑1). After treatment at various concentrations (1‑200 µM for 72 h), effect on proliferation types varied; these effects were found to be associated ERα expression. Daidzein exhibited biphasic...

Genetic mutations disrupting the structure and function of primary cilia cause various inherited retinal diseases in humans. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, pleiotropic ciliopathy characterized by degeneration, obesity, postaxial polydactyly, intellectual disability, genital renal abnormalities. To gain insight into mechanisms degeneration BBS, we developed congenital knockout mouse Bbs8, as well conditional models which BBSome (a protein complex that mediates...

Abstract Diabetes, a chronic metabolic disorder, is characterized by persistent hyperglycemia. This study aimed to evaluate the hypoglycemic and antioxidant activities of lactic acid bacteria strains isolated from humans food products investigate probiotic properties selected four strains. The activity was examined evaluating α-glucosidase α-amylase inhibitory activities. measured using DPPH, ABTS, FRAP assays. Four ( Lactiplantibacillus plantarum MG4229, MG4296, MG5025, Lacticaseibacillus...

Abstract Luteolin (3′,4′,5,7‐tetrahydroxyflavone), a flavonoid, has been known to possess antimutagenic, antitumorigenic, antioxidant, and anti‐inflammatory properties. In this study, we investigated the role of luteolin in regulation adipogenic differentiation 3T3‐L1 preadipocytes. inhibited intracellular triglyceride accumulation dose‐dependent manner without cytotoxicity. Western blot reverse transcription‐polymerase chain reaction analyses showed that inhibition was accompanied by...

To investigate the anticancer, anti-inflammatory, and antiobesity activity of methanol extracts eight distinct species: Artemisia Stolonifera (AST), Selengensis (ASE), Japonica, Montana, Capillaris (ACA), Sylvatica (ASY), Keiskeana (AKE), Scoparia (ASC) in vitro. Antiproliferative was investigated human breast cancer estrogen receptor-a positive T47D negative HS578T cell lines exposed to at various concentrations (5-200 mg/mL) for 24, 48, 72 h. For evaluating anti-inflammatory extracts,...

The present study investigated the effect of phytochemical genistein on proliferation and differentiation MCF-7 3T3-L1 cells via regulation estrogen receptor-α (ERα) expression induction apoptosis. When human breast cancer were treated with 50, 100, 150 200 µM for 24, 48 or 72 h, cell growth was significantly decreased in a concentration-dependent manner. Notably, patterns ERα similar. Furthermore, differentiating inhibited by h treatment 50 genistein, which selected based results...

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In this study, lactic acid bacteria (LAB) strains derived from human and fermented food sources were examined to identify their properties related obesity, as well establish safety stability probiotics. LAB (Lacticaseibacillus rhamnosus MG4502, Lactobacillus gasseri MG4524, Limosilactobacillus reuteri MG5149, Weissella cibaria MG5285) exhibited antioxidant activity through DPPH (>26.1%) ABTS (>40.1%) radical scavenging assays α-glucosidase inhibitory activities (>60.3%),...

This study was performed to investigate the potential use of Saussurea lappa C.B. Clarke as a source anti-oxidant agents. Various solvent fractionates from S. roots were investigated for their anti-oxidative effectiveness. The contents total phenolics and flavonoids determined by Folin-Ciocalteu's colorimetric aluminum nitrate method, respectively. Total phenolic flavonoid n-butanol soluble Clarke, 44.43 μg gallic acid equilibrium (GAE)/g extract 92.15 quercetin (QE)/g extract, respectively,...

Ecklonia cava, an edible marine brown alga (Laminariaceae), is a rich source of phlorotannins. This study aimed to investigate the anti-inflammatory effect cava ethanol extract (ECE, dieckol 10.6%, w/w) on Porphyromonas gingivalis lipopolysaccharide-stimulated inflammation in RAW 264.7 cells and ligature-induced periodontitis rats. The levels nitric oxide (NO) prostaglandin E2 were decreased by more than half treatment with 100 μg/mL ECE. Downregulated tumor necrosis factor-α, interleukin...

Indoleamine 2,3-dioxygenase 1 (IDO1), a tryptophan catabolising enzyme, is known as tumour cell survival factor that causes immune escape in several types of cancer. Flavonoids Sophora flavescens have variety biological benefits for humans; however, cancer immunotherapy effect has not been fully investigated. The flavonoids (1-6) isolated from S. showed IDO1 inhibitory activities (IC50 4.3-31.4 µM). representative (4-6) were determined to be non-competitive inhibitors by kinetic analyses....

Excessive consumption of dietary fat is closely related to obesity, diabetes, insulin resistance, cardiovascular disease, hypertension, and non-alcoholic fatty liver disease. Recently, probiotics have been highly proposed as biotherapeutic treat prevent diseases. Previously, there are studies that demonstrated the beneficial effects against metabolic disorders, including obesity diabetes.We investigated anti-obesity effect mechanism action four human-derived lactic acid bacterial (LAB)...

In this study, we examined the anti-tumor effects of heat-killed Bifidobacterium and Lactobacillus strains on human colorectal carcinoma RKO cells in vitro vivo xenograft models. First, cytotoxic apoptotic 11 different were using an MTT assay flow cytometry, respectively. Then, BALB/c nude mice implanted with orally administered single or mixed bacterial to examine their inhibitory tumor growth. Additionally, levels cleaved caspase-9, -3, -7 PARP tissues analyzed Western blotting...

Glucosinolates, precursors of isothiocyanates, are present in cruciferous vegetables such as the turnip (Brassica rapa L.). Glucosinolates usually broken down through hydrolysis catalyzed by myrosinase released from damaged plant cells. and their breakdown products, particular have long been known to various pharmaceutical benefits, including anticarcinogenic, antimicrobial antioxidant properties. In this study, quantitative analyses isothiocyanates total glucosinolates turnip, which was...