A5008352017- Advanced Biosensing Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Near-Field Optical Microscopy
- Biosensors and Analytical Detection
- Molecular Communication and Nanonetworks
- Cancer Cells and Metastasis
- Advanced biosensing and bioanalysis techniques
- Force Microscopy Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Shape Memory Alloy Transformations
- Ultrasonics and Acoustic Wave Propagation
- Advanced Proteomics Techniques and Applications
- Acoustic Wave Resonator Technologies
Max Planck Institute for the Science of Light
2019-2023
University of Tehran
2015-2017
Abstract Interferometric scattering (iSCAT) microscopy is a label-free optical method capable of detecting single proteins, localizing their binding positions with nanometer precision, and measuring mass. In the ideal case, iSCAT limited by shot noise such that collection more photons should extend its detection sensitivity to biomolecules arbitrarily low However, number technical sources combined speckle-like background fluctuations have restricted limit in iSCAT. Here, we show an...
An integrated nano-electromechanical chip (NELMEC) has been developed for the label-free distinguishing of both epithelial and mesenchymal circulating tumor cells (ECTCs MCTCs, respectively) from white blood (WBCs). This nanoelectronic microfluidic fabricated by silicon micromachining can trap large single (>12 µm) at opening analysis microchannel arrays. The nature captured is detected using nanograss (SiNG) electrodes patterned entrance channels. There an observable difference between...
It has been shown that interferometric detection of Rayleigh scattering (iSCAT) can reach an exquisite sensitivity for label-free nano-matter, down to single proteins. The iSCAT is intrinsically limited by shot noise, which be indefinitely improved employing higher illumination power or longer integration times. In practice, however, a large speckle-like background and technical issues in the experimental setup limit attainable signal-to-noise ratio. Strategies algorithms data analysis are,...
Abstract Interferometric scattering (iSCAT) microscopy is a label-free optical method capable of detecting single proteins, localizing their binding positions with nanometer precision, and measuring mass. In the ideal case, iSCAT limited by shot noise so that collection more photons should allow its detection sensitivity to biomolecules arbitrarily low However, number technical sources combined speckle-like background fluctuations have restricted limit in iSCAT. Here, we show an unsupervised...
In this paper, a thermally actuated medical stent and staple using shape memory alloy (SMA) are designed, numerically analysed, compared to similarly designed stents staples made of 316L stainless steel Co-Cr alloy. The numerical analysis was carried out finite element method. A consistent 3D model is used investigate the effect materials on performance staple. This study takes into account interaction between stent/staple in vivo environment, by modelling environment forces. results good...
Abstract It has been shown that interferometric detection of Rayleigh scattering (iSCAT) can reach an exquisite sensitivity for label-free nano-matter, down to single proteins. The iSCAT is intrinsically limited by shot noise, which be indefinitely improved employing higher illumination power or longer integration times. In practice, however, a large speckle-like background and technical issues in the experimental setup limit attainable signal-to-noise ratio. Strategies algorithms data...
Interferometric scattering (iSCAT) microscopy detects single nanoparticles in a label-free fashion. Utilizing self-supervised machine learning pushes the detection sensitivity of iSCAT to very small proteins and disease markers such as chemokines cytokines.
Proteins are involved in a large number of biological processes. Apart from the field proteomics, where ensemble studies entire set proteins present system carried out, detection and analysis single has become vibrant research. Particularly, study that secreted cells into extracellular space is an important topic with wide implications for basic intercellular interactions immunology. Secretory responsible vast amount cellular functions involving migration, wound healing, immunological...
In recent decades large efforts have been made to push optical imaging the limit, for example detecting very small proteins. Great achievements obtained with fluorescence microscopy, both in terms of localization precision and study dynamics different molecules. Despite constant improvements, fluorescence-based methods suffer from intrinsic limitations, such as finite number emitted photons photo damage, which limits temporal resolution duration a measurement. Furthermore, if molecule...
We use interferometric scattering microscopy (iSCAT) to study in-situ secretion dynamics of living cells. The method allows us optically detect single proteins in real-time without the need for labeling.