A5091243074- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Transgenic Plants and Applications
- Asthma and respiratory diseases
- Research on Leishmaniasis Studies
- T-cell and B-cell Immunology
- Contact Dermatitis and Allergies
- Biochemical Analysis and Sensing Techniques
- Toxin Mechanisms and Immunotoxins
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Burkholderia infections and melioidosis
- Lysosomal Storage Disorders Research
- Psoriasis: Treatment and Pathogenesis
- Mast cells and histamine
- Drug-Induced Adverse Reactions
- vaccines and immunoinformatics approaches
- Trypanosoma species research and implications
- Immunodeficiency and Autoimmune Disorders
Monash University
2010-2023
Alfred Health
2023
The Alfred Hospital
2010-2017
Marine Biological Laboratory
2006
London School of Hygiene & Tropical Medicine
2005-2006
Imperial College London
2005-2006
Wellcome Trust
2005-2006
University of London
2005
Summary Background Peanut allergy is a life‐threatening condition; there currently no cure. While whole allergen extracts are used for specific immunotherapy many allergies, they can cause severe reactions and even fatalities in peanut allergy. Objective To identify short, HLA‐degenerate CD4 + T cell epitope‐based peptides of the major Ara h 1 that target allergen‐specific cells without causing IgE‐mediated inflammatory activation, as candidates safe peanut‐specific immunotherapy. Methods...
Abstract Background Food allergy is a leading cause of anaphylaxis worldwide. Allergen‐specific immunotherapy the only treatment shown to modify natural history allergic disease, but application food has been hindered by risk severe reactions and short‐lived efficacy. Allergen‐derived peptides could provide solution. PVX108 comprises seven short representing immunodominant T‐cell epitopes major peanut allergens for allergy. Methods Pre‐clinical safety was assessed using ex vivo basophil...
CD8+ T cells have a protective role in experimental visceral leishmaniasis. However, the observation that inflammatory cytokines induce bystander activation of questions need for antigen-dependent effector function. Here, we demonstrate successful adoptive immunotherapy with is strictly dependent upon presence cognate antigen.
The antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype is topic current controversy. We aimed to determine whether human can be induced express MHC Class II act as antigen presenting cells for T cell stimulation. Isolated were exposed panel cytokines TLR-ligands assessed expression. was expressed up 17% isolated following incubation with combination IL-3, IFN-γ GM-CSF 72 hours. Costimulatory molecules (CD80 CD86) at very low levels after Gene expression...
Summary Background Peanut allergy causes severe type 1 hypersensitivity reactions and conventional immunotherapy against peanut is associated with a high risk of anaphylaxis. Objective Our current study reports proof concept experiments on the safety stably denatured variant major allergen Ara h 2 for immunotherapy. We determined impact structure loss its IgE binding basophil degranulation capacity, T cell reactivity as well anaphylactic potential. Methods The secondary untreated...
The incidence of obesity has risen to epidemic proportions in recent decades, most commonly attributed an increasingly sedentary lifestyle, and a 'western' diet high fat low fibre. Although non-allergic asthma is well-established co-morbidity obesity, the influence on allergic still under debate. Allergic thought result from impaired tolerance airborne antigens, so-called respiratory tolerance. We sought investigate whether fats affects development Mice fed (HFD) for 8 weeks showed weight...
<b><i>Background:</i></b> Bahia grass pollen (BaGP) is a major cause of allergic rhinitis. Subcutaneous allergen-specific immunotherapy effective for allergy, but unsuitable patients with moderate to severe asthma due the risk anaphylaxis. T cell-reactive IgE nonreactive peptides provide safer treatment option. This study aimed identify and characterize dominant CD4<sup>+</sup> cell epitope BaGP allergen, Pas n 1....
<i>Background:</i> Peanut and tree nut allergies are life-threatening conditions for many affected individuals worldwide. Currently there is no cure. While co-allergy to peanut nuts a common clinical observation, IgE cross-reactivity between reported, T cell poorly defined. <i>Methods:</i> Hazelnut-specific lines were established using peripheral blood mononuclear cells from 5 subjects with hazelnut peanut. These stimulated extracts purified major allergens, Ara h 1...
Abstract The importance of the site Ag localization within microbial pathogens for effective generation CD8+ T cells has been studied extensively, generally supporting view that secretion infected target is required optimal MHC class I-restricted presentation. In contrast, relatively little known about pathogen activation II-restricted CD4+ cells, despite their clear host protection. We have used N-terminal targeting sequence Leishmania major hydrophilic acylated surface protein B to...
ABSTRACT It has been proposed that long-lived memory T cells generated by vaccination or infection reside within a compartment finite size. Consequently, in variety of acute models interclonal competition shown to lead attrition preexisting CD8 + cells. Contrary expectations, therefore, we found chronic Leishmania donovani Listeria -immune mice results heightened protection against subsequent challenge. This was associated with bystander expansion -specific and bias these toward central...
Allergic diseases including asthma, rhinitis and eczema are known to be a major health economic burden worldwide. Specific immunotherapy (SIT) is potentially curative but restricted in use, e.g. for asthmatics, due risk of serious adverse events. Safer, effective SIT preparations require elucidation mechanisms immunoregulatory factors. Allergen-specific T cells play pivotal role. For allergic individuals, allergen-stimulated largely secrete IL-4, IL-5 IL-13 (Th2-type cytokines), whereas...