The culture conditions found to result in stable proliferation of normal rat hepatocytes are: (i) subconfluent cell densities; (ii) serum-free medium; (iii) hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other factors; (iv) substrata liver extracellular matrix depleted inhibitors. Serum was deleterious parenchymal cells: it inhibitory the expression liver-specific functions, cytostatic cells at all seeding densities, cytotoxic them low...

Fast myosin light chains (MLCf) 1 and 3 are proteins associated with the thick filament in vertebrate fast muscle fibers. MLC1f MLC3f have complete sequence homology for first 141 amino acids from their COO- end, but they differ length acid at NH+3 ends (MLC1f = 49 acids, 8 acids), translated different mRNAs. To elucidate structural relationship between mammalian chain isoforms, cDNA clones been isolated rat skeletal primary nucleotide sequences were determined. mRNAs display identity 3'...

We have characterized cDNAs coding for three Na,K-ATPase alpha subunit isoforms from the rat, a species resistant to ouabain. Northern blot and S1-nuclease mapping analyses revealed that these mRNAs are expressed in tissue-specific developmentally regulated fashion. The mRNA 1 isoform, approximately equal 4.5 kb long, is all fetal adult rat tissues examined. 2 mRNA, also predominantly brain heart. 3 cDNA detected two species: present most 6 found only brain, heart, skeletal muscle. deduced...

We have previously isolated and characterized cloned complementary DNAs (cDNAs) for striated smooth muscle a-tropomyosin.The sequences of these cDNA clones suggested that two isoforms were encoded by the same gene.Here, we determined complete structure a-tropomyosin (a-TM) gene, establishing a single with sequence complexity 28 kilobase pairs, is split into 12 exons produces a-TM mRNA alternative splicing minimum five exchangeable isotype-specific exons.The elucidation intronlexon...

Sodium, potassium-adenosine triphosphatase (Na + ,K -ATPase) is hypothesized to be involved in systemic vascular hypertension through its effects on smooth muscle reactivity and myocardial contractility. By means of RNA blot analyses cardiac, aortic, skeletal RNAs two rat hypertensive models, Na -ATPase α-subunit messenger isoforms (α2 α3) were shown deinduced response increased intravascular pressure. The changes observed after 48 hours or more experimental hypertension. Under these...

Abstract —A link between prenatal malnutrition and hypertension in human populations has recently been proposed. Rat models of have provided major support for this theory on the basis tail-cuff measurements. However, technique requires restraint elevated temperature, both potential sources stress. To determine effect protein blood pressure under nonstress conditions, 24-hour radiotelemetric measurements were taken home cage. Male rats born to dams fed a 6% casein diet 5 weeks before mating...

Serum-free, hormonally defined media have been developed for optimal growth of a rat hepatoma cell line. The cells' hormonal requirements are dramatically altered both qualitatively and quantitatively by whether they were plated onto tissue culture plastic or collagenous substrata. On substrata, the cells required insulin, glucagon, hormone, prolactin, linoleic acid (bound to BSA), zinc, copper, selenium. For on plastic, above factors at higher concentrations plus several additional factors:...

The sodium- and potassium-dependent adenosine triphosphatase (Na + ,K -ATPase) maintains the transmembrane Na gradient to which is coupled all active cellular transport systems. R S alleles of gene encoding -ATPase α1 subunit isoform were identified in Dahl salt-resistant (DR) salt-sensitive (DS) rats, respectively. Characterization allele-specific complementary DNA a leucine substitution glutamine at position 276. This mutation alters hydropathy profile region proximity T 3 (Na),...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCloning and tissue distribution of rat heart fatty acid binding protein mRNA: identical forms in skeletal muscleKevin P. Claffey, Victoria L. Herrera, Peter Brecher, Nelson Ruiz-OpazoCite this: Biochemistry 1987, 26, 24, 7900–7904Publication Date (Print):December 1, 1987Publication History Published online1 May 2002Published inissue 1 December 1987https://pubs.acs.org/doi/10.1021/bi00398a054https://doi.org/10.1021/bi00398a054research-articleACS...

A current challenge in the treatment of peritoneal carcinomatosis is inability to detect, visualize, and resect small or microscopic tumors pancreatic, ovarian, mesothelial origin. In these diseases, completeness primary tumor resection directly correlated with patient survival, hence, identifying sub-millimeter (i.e., disseminated disease) critical. Thus, new imaging techniques probes are needed improve cytoreductive surgery outcomes. Highly fluorescent rhodamine-labeled expansile...

A rat albumin cDNA probe (pBR alb 149) was developed in order to investigate the molecular mechanisms responsible for changes hepatic protein synthesis after chronic administration of ethanol rats. Rats fed a diet up 1 year which 36% calories were from ethanol, fatty livers but not cirrhosis. Cell-free with liver membrane-bound polysomes ethanol-fed rats increased as compared control polysomes, whereas free unchanged. Total RNA extracted and translated rabbit reticulocyte mRNA-dependent...

SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees illness death. A better understanding the immune response and subsequent, often excessive, inflammation may inform treatment decisions reveal opportunities for therapy. We studied cell subpopulations their associations with clinical parameters cohort 26 patients COVID-19. Following informed consent, we collected blood samples hospitalized COVID-19 within 72 h admission. Flow cytometry was used...

Despite the prevalence of essential hypertension, its underlying genetic basis has not been elucidated due to complexities determinants. To identify a hypertension susceptibility gene, we used an approach that integrates molecular, transgenic, and analysis using Dahl salt-sensitive (S) salt-resistant (R) rats ascertained for genotype phenotype. determine role S Q276L alpha1 Na,K-ATPase gene variant, developed transgenic bearing R wild-type (wt) Na, K-ATPase cDNA directed by cognate wt...

Na(+)-K(+)-activated adenosine triphosphatase (Na(+)-K(+)-ATPase) is the integral membrane protein that maintains Na(+)-K(+) electrochemical gradient across plasma membrane. Because of importance to fundamental and specialized cell functions, we investigated cell-specific modulation Na(+)-K(+)-ATPase alpha-subunit isoform (alpha 1, alpha 2, 3) gene expression in different stages postimplantation mouse embryos neonatal rat tissues by situ hybridization with use isoform-specific rat-derived...

Years after infection with hepatitis B virus, chimpanzees may have manifestations of the carrier state as described in man. In addition to serologic evidence for persistent viral infection, percutaneous liver biopsy specimens showed virus surface antigen cytoplasm and core nucleus. Four animals had portal inflammatory reaction seen human chronic hepatitis. Viral DNA was demonstrated nucleic acid extracts by molecular hybridization cloned plasmic pA01 containing sequences. Although a molecule...

Alzheimer's disease (AD) is characterized by increased β amyloid (Aβ) levels, extracellular Aβ deposits in senile plaques, neurofibrillary tangles, and neuronal loss. However, the physiological role of normal levels its parent protein, precursor protein (APP) are unknown. Here we report that low-level transgenic (Tg) expression Swedish APP mutant gene (APPswe) Fischer-344 rats results attenuated age-dependent cognitive performance decline 2 hippocampus-dependent learning memory tasks...

Essential hypertension remains a major risk factor for cardiovascular and cerebrovascular diseases. As complex multifactorial disease, elucidation of susceptibility loci elusive. ATP1A1 Dear are candidate genes 2 closely linked rat chromosome-2 blood pressure quantitative trait loci. Because corresponding human syntenic regions on different chromosomes, investigation (chromosome [chr]-1p21) (chr-4q31.3) facilitates genetic analyses each locus in hypertension. Here we report the association...

Multiple clinical studies show that arterial stiffness, measured as pulse wave velocity (PWV), precedes hypertension and is an independent predictor of end organ diseases including stroke, cardiovascular disease chronic kidney disease. Risk factor for stiffness implicate age, sodium. However, causal mechanisms linking risk to remain be elucidated. Here, we studied the relationship in Na-induced, stroke-prone Dahl salt-sensitive (S) hypertensive rat model, analyzed putative molecular...