A5072616965- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Cancer Genomics and Diagnostics
- Carcinogens and Genotoxicity Assessment
- Endoplasmic Reticulum Stress and Disease
- Genetic factors in colorectal cancer
- Liver Disease Diagnosis and Treatment
- Bee Products Chemical Analysis
- Colorectal and Anal Carcinomas
- Prostate Cancer Treatment and Research
- Viral Infectious Diseases and Gene Expression in Insects
- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Molecular Biology Techniques and Applications
- Prostate Cancer Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Microbial Inactivation Methods
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Air Quality and Health Impacts
- Liver physiology and pathology
- Veterinary Equine Medical Research
- Toxin Mechanisms and Immunotoxins
The Maria Sklodowska-Curie National Research Institute of Oncology
2006-2023
Expression of constitutively active heat shock transcription factor 1 (HSF1) in mouse spermatocytes induces apoptosis and leads to male infertility. We report here that prior the onset massive caused by expression HSF1 a marked reduction spermatocyte‐specific Hsp70.2 mRNA protein levels occurs. In addition, HSP70.2 relocalizes from predominant cytoplasmic nuclear position developing express HSF1. Later developmental stages, cells undergoing HSF1‐induced essentially lack protein. The...
The human HSPA2 gene, which belongs to the HSP70 family of heat shock genes, is a counterpart rodent testis-specific HspA2 gene. Rodent genes are expressed mainly in pachytene spermatocytes, while transcripts gene have been detected various normal somatic tissues, albeit translation messenger RNA into corresponding protein has not yet unambiguously demonstrated, except for several cancer cell lines. aim our work, first step search function cells, was establish its intracellular localization...
The present report is a follow-up to our previous molecular epidemiology studies on DNA damage in residents of the industrial region Upper Silesia. study was designed focus environmental exposure airborne pollutants; other exposures or confounding factors (e.g. smoking status and age) were eliminated. A Silesian population consisting 67 donors compared 72 inhabitants less polluted but similarly urbanized area, surrounded by rural part Poland. In both regions non-smoking females with similar...
The precise analysis of tumour markers in blood such as circulating cell-free DNA (cfDNA) could have a significant impact facilitating monitoring patients after initial therapy. Although high levels total cfDNA plasma cancer are consistently demonstrated, low sensitivity alterations is reported.The major question regards the recovery tumour-specific KRAS mutated and cancer-associated type 16 human papillomavirus (HPV16).TaqMan technology was used for detection mutation, HPV16 to quantify...
Heat shock activates in somatic cells a set of genes encoding heat proteins which function as molecular chaperones. The basic mechanism by these are activated is the interaction specific transcription factor HSF1 with regulatory DNA sequence called element (HSE). In higher eukaryotes present unstressed inactive monomers which, response to cellular stress, aggregate into transcriptionally competent homotrimers. paper we showed that expression transgene mutated constitutively active placed...
Abstract To determine whether DNA transfer to mouse testes by in vivo electroporation could be useful method for studying regulatory elements of genes specifically active spermatocytes first we compared the expression pattern a construct containing EGFP reporter gene ligated fragment heat shock testis‐specific hst70 promoter, both testis transgenic mice and electroporated vivo. While was expressed all seminiferous tubules cell‐ stage‐specific manner, only small fraction cells this marker...
Abstract Accumulation of inducible heat shock proteins (e.g. Hsp70i) during cellular stress confers thermotolerance, reduces the consequences damage and facilitates recovery, while abrogation Hsp70i expression renders sensitivity to apoptosis. Testis translocation into abdominal cavity, which results in temperature elevation, does not induce proteins. Despite constitutive testis‐specific Hsp70 proteins, spermatocytes are very sensitive at elevated temperatures. To test whether protein could...
Abstract Somatic mutations of 11q23.3‐linked constitutive heat shock protein 70 gene ( HSPA8 alias HSC70 ) are detected by others in breast carcinomas. To examine whether intragenic, somatic occur lung carcinomas, we sequenced its exons 2–8, with adjacent intronic sequences, a series DNA samples from non‐small‐cell cancers (NSCLC). Twenty‐one polymorphisms were detected, but no mutation. However, observed an association between the 1541‐1542delGT genotype and immunohistochemical staining...
Background: Implementation of anal squamous cell carcinoma (ASCC) treatment modifications requires reliable patient risk stratification. The circulating tumor–related human papillomavirus type 16 (ctHPV16) may play a role in predicting survival or assessing response. Methods: study included 62 ASCC patients treated with chemoradiotherapy. A threshold 2.5 was used to determine the maximum standardized uptake value (SUVmax). ctHPV16 viral load (VL) quantified by qPCR. Results: In multivariate...
The p53 mutation spectrum can generate hypotheses linking carcinogen exposure to human cancer. Although it is well-documented that tobacco smoking a major cause of lung cancer, the contribution air pollution less well-established. We determined molecular and immunohistochemical changes (p53 gene mutations, protein accumulation WAF1 expression) genetic polymorphisms GSTM1, CYP1A1 CYP2D6 genes in case series non-small-cell cancers from Silesia. This region southern Poland highly industrialized...
Stress-inducible Hsp70i and constitutively expressed Hsc70 are highly related heat shock proteins. Aberrant expression levels intracellular localization of these proteins has been suggested as a potential marker in certain tumors. The aim our study was to work out reliable, immunohistochemical detection the stress-inducible protein enabling discrimination between paraffin-embedded human tissues. We tested effect several fixative procedures antigen retrieval on effectiveness murine cells...
Human papilloma virus (HPV) is highly frequent among patients with anal squamous cell carcinoma, but the viral load (VL) differs between patients. This study aimed to compare rate of HPV positivity, HPV16VL, p16INK4A and p53 expression treatment naive recurrent cancer was genotyped via AmpliSens® HCR-genotype-titre-FRT kit. HPV16 VL determined quantitative polymerase chain reaction-based in-house test. tested immunohistochemistry. The cohort comprised 13 treatment-naive 17 SCC High-risk...
Fine needle aspiration (FNA) is routine diagnostic tool in breast tumors assessment. In some cases, however, limitations of this method do not permit an unequivocal diagnosis. these, suspected, cases immunocytochemical evaluation selected biological markers may be help. The aim the study was assessment HSP27 value diagnosis and discrimination benign malignant lesions. expression examined by immunocytochemistry fine smears assessed to C2-C5 categories. C5 subgroup correlated with ER, PR...
Analysis of circulating cell-free DNA in blood is considered as a liquid biopsy, which enables non-invasive and repetitive investigation the tumor DNA. The potential clinical usefulness frequently examined lung cancer. Thus, our aim was assessment if chemotherapy influences concentration.Fifty-seven cancer patients advanced stages disease were examined. Quantification determined by TERT amplification.Distant metastases significantly connected with level. Patients treated conventional had...
Ewa Malusecka, Anna Fiszer-Kierzkowska, Robert Herok, Stanislaw Wronski and Justyna Rembak-Szynkiewicz-Combining Laser-Assisted Microdisstection with/and Immunohistochemistry - RNA Quality of Clinical LCM-Derived Samples