A5067889239- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Marine animal studies overview
- Ion Channels and Receptors
- Ion channel regulation and function
- Arctic and Antarctic ice dynamics
- Lipid Membrane Structure and Behavior
- Asthma and respiratory diseases
- Biomedical Research and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cephalopods and Marine Biology
- Underwater Acoustics Research
- Dementia and Cognitive Impairment Research
- Biomedical Text Mining and Ontologies
- Insect and Arachnid Ecology and Behavior
- Peptidase Inhibition and Analysis
- Computational Drug Discovery Methods
- Nanoparticle-Based Drug Delivery
- Nuclear Receptors and Signaling
- Bat Biology and Ecology Studies
- Cerebrovascular and genetic disorders
- Intracerebral and Subarachnoid Hemorrhage Research
- Supramolecular Self-Assembly in Materials
- 14-3-3 protein interactions
Marine Ecology and Telemetry Research
2022
Calvin University
2019
Glenfield Hospital
2014-2015
University of Leicester
2014-2015
Cornell University
1995-2004
Institut thématique Génétique, génomique et bioinformatique
2001
Rockefeller University
1996-1997
Johns Hopkins University
1997
Hospital for Special Surgery
1995
Tufts University
1990
To study the metabolism of amyloid beta protein (Abeta) in Alzheimer's disease, we have developed a new approach for analyzing profile soluble Abeta and its variants. In present method, variants are immuno-isolated with Abeta-specific monoclonal antibodies. The identities determined by measuring their molecular masses using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. levels relative peak intensities spectrometric measurements comparison internal standards...
β-amyloid protein (Aβ) formation was reconstituted in permeabilized neuroblastoma cells expressing human Alzheimer precursor (βAPP) harboring the Swedish double mutation associated with familial early-onset disease. Permeabilized were prepared following metabolic labeling and incubation at 20°C, a temperature that allows βAPP to accumulate trans-Golgi network (TGN) without concomitant Aβ formation. Subsequent 37°C led generation of Aβ. production TGN persisted even under conditions which...
Fluorometric and paper chromatographic evidence indicates that dopamine is the only catecholamine present in ganglia of a number lamellibranch gastropod species.
Phospholipase D (PLD) hydrolyzes phosphatidylcholine to generate phosphatidic acid. In mammalian cells this reaction has been implicated in the recruitment of coatomer Golgi membranes and release nascent secretory vesicles from trans-Golgi network. These observations suggest that PLD is associated with complex; however, date, because its low abundance, intracellular localization characterized only indirectly through overexpression chimeric proteins. We have used highly sensitive antibodies...
Abstract Inheritance of the apolipoprotein E (ApoE) ϵ4 allele is a risk factor for Alzheimer's disease (AD) and associated with increased deposition β‐amyloid (Aβ) in AD, Down's syndrome, normal aging. Aβ form senile plaques (SPs) has recently been described patients temporal lobe epilepsy (TLE). We studied relationship between ApoE genotype tissue from who underwent lobectomy intractable epilepsy. TLE SPs had 70% carrier frequency compared 27% among age‐matched controls without SPs. Our...
Various compounds that affect signal transduction regulate the relative utilization of alternative processing pathways for beta-amyloid precursor protein (beta APP) in intact cells, increasing production nonamyloidogenic soluble beta APP (s and decreasing amyloidogenic peptide. In a recent study directed toward elucidating mechanisms underlying phorbol ester-stimulated s secretion from it was demonstrated kinase C increases formation trans-Golgi network (TGN) APP-containing secretory...
Recent work from our laboratory demonstrated that phosphatidic acid (PA) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P<sub>2</sub>), are required to maintain the structural integrity of Golgi apparatus. To investigate role these lipids in regulating structure function, we developed a novel assay follow release post-Golgi vesicles. Isolated rat liver membranes were incubated with [<sup>3</sup>H]CMP sialic radiolabel endogenous soluble membrane glycoproteins present late trans-Golgi...
Most early-onset familial Alzheimer disease is associated with missense mutations in S182, a membrane protein on chromosome 14. We investigated amyloid-beta (A beta) precursor beta PP) metabolism skin fibroblasts from S182 (Glu246)-affected individuals and unaffected family members. Steady-state A PP levels were similar among all lines as was the degree of increase soluble released upon stimulation cells either phorbol ester or serum. Among studied, consistently detectable only medium single...
The desire of animal behaviorists for more flexible methods conducting inter-study and inter-specific comparisons meta-analysis various behaviors compelled us to design an automated, behavior peak detection method that is potentially generalizable a wide variety data types, animals, behaviors. We detected the times feeding attempts by 12 Risso's dolphins (Grampus griseus) 36 blue whales (Balaenoptera musculus) using norm-jerk (rate change acceleration) time series. automated algorithm...
Asthma is characterized by airway hyper-responsiveness and variable airflow obstruction, in part as a consequence of hyper-contractile smooth muscle, which persists primary cell culture. One potential mechanism for this hyper-contractility abnormal intracellular Ca2+ handling. We sought to compare handling muscle cells from subjects with asthma compared non-asthmatic controls measuring: i) bradykinin-stimulated changes inositol 1,4,5-trisphosphate (IP3) accumulation concentration, ii)...
Knowledge of Cuvier’s beaked whale Ziphius cavirostris behavior has expanded through the utilization animal-borne tags. However, many tag types do not record sound—thus preventing echolocation click detections to identify foraging—or have short deployments that sample a limited range behaviors. As quantity such non-acoustic data grows, so too does need for robust methods detecting foraging from data. We used 692 dives 5 sound-recording tags on whales in southern California, USA, develop...
Risso’s dolphins ( Grampus griseus ), uncommon prior to the 1970’s, are now regularly observed within Southern California Bight. During long-term cetacean monitoring programs on United States Navy range areas in Bight from 2009–2019, we deployed 16 Argos-linked satellite tags acquire objective, detailed depictions of their movements and behaviors. Individuals were tracked for a median 10.7 days (range = 0.8 – 19.7). Kernel density estimation suggested individuals utilized entire with 50%...
Goose-beaked whales ( Ziphius cavirostris ) are deep-diving cetaceans known for their elusive nature and specialized foraging behavior. In 2019 2020, six telemetry tags were deployed on these in Southern California, resulting 395 h of acoustic diving data. Foraging dives manually identified by the presence echolocation pulses buzzes, generalized additive models assessed factors influencing The median bathymetric depth at sites was 1,419 m (IQR = 359), maximum dive highly correlated with...
The apolipoprotein E epsilon4 allele contributes to the genetic susceptibility underlying a large proportion (~40-60%) of typical, sporadic Alzheimer disease. Apolipoprotein deficient mice made transgenic for human accumulate excess cerebral amyloid when compared similarly prepared expressing epsilon3. Therefore, it is important search relevant interactions(s) between and Abeta in order clarify biological role Using thioflavine T (ThT)-based assay, we have investigated effects native...