A5079546505- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Parallel Computing and Optimization Techniques
- Distributed and Parallel Computing Systems
- Plant Gene Expression Analysis
- Nuclear Physics and Applications
- Plant Virus Research Studies
- Laser-Plasma Interactions and Diagnostics
- Radiation Therapy and Dosimetry
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Advanced Computational Techniques and Applications
- Plant Reproductive Biology
- Plant responses to water stress
- Aquatic Invertebrate Ecology and Behavior
- Plant Pathogenic Bacteria Studies
- Soybean genetics and cultivation
- Phytoplasmas and Hemiptera pathogens
- Topic Modeling
- Geochemistry and Geochronology of Asian Mineral Deposits
- Fungal and yeast genetics research
- Data Quality and Management
- NF-κB Signaling Pathways
Central South University of Forestry and Technology
2021-2024
Central South University
2021-2024
Sichuan Academy of Agricultural Sciences
2024
Xidian University
2023
Nanjing Agricultural University
2023
Lawrence Berkeley National Laboratory
2022
Center for Excellence in Molecular Plant Sciences
2018-2022
Chinese Academy of Sciences
2015-2022
Sichuan University
2022
Shandong University
2022
Abstract A contribution of DNA methylation to defense against invading nucleic acids and maintenance genome integrity is uncontested; however, our understanding the extent involvement this epigenetic mark in genome-wide gene regulation plant developmental control incomplete. Here, we knock out all five known methyltransferases Arabidopsis , generating methylation-free plants. This quintuple mutant exhibits a suite defects, unequivocally demonstrating that essential for multiple aspects...
Abstract Epigenetic variation has been proposed to facilitate adaptation changing environments, but evidence that natural epialleles contribute adaptive evolution lacking. Here we identify a retrotransposon, named “NMR19” (naturally occurring DNA methylation region 19), whose and genomic location vary among Arabidopsis thaliana accessions. We classify NMR19 as NMR19-4 NMR19-16 based on its location, uncover an epiallele controls leaf senescence by regulating the expression of PHEOPHYTIN...
Abstract Radiotherapy is the current standard of care for more than 50% all cancer patients. Improvements in radiotherapy (RT) technology have increased tumor targeting and normal tissue sparing. Radiations at ultra-high dose rates required FLASH-RT effects sparked interest potentially providing additional differential therapeutic benefits. We present a new experimental platform that first one to deliver petawatt laser-driven proton pulses 2 MeV energy 0.2 Hz repetition rate by means...
It is challenging to determine the effect of DNA methylation on epigenetic landscape and function in higher organisms due lack methylation-free mutants.
Abstract Bathymodiolid mussels dominate hydrothermal vents, cold methane/sulfide-hydrocarbon seeps and other sites of organic enrichment. Here, we aimed to explore the innate immune system detoxification mechanism deep sea mussel Bathymodiolus platifrons collected from a methane seep in South China Sea. We sequenced transcriptome mussels’ gill, foot mantle tissues generated transcriptomic database containing 96,683 transcript sequences. Based on GO KEGG annotations, reported transcripts that...
Significance The epigenome influences gene regulation and genome evolution. DNA methylomes of Arabidopisis hybrids are distinct from both parents; however, how the parental interact in is poorly understood. We discovered pervasive, nonadditive methylation changes (“methylation interactions”) throughout Col C24 Arabidopsis accessions. Methylation interactions correlated with high levels small interfering RNAs, known components RNA-directed (RdDM) pathway. Indeed, abrogation RdDM activity...
In plants, establishment of de novo DNA methylation is regulated by the RNA-directed (RdDM) pathway. RdDM machinery known to concentrate in Cajal body, but biological significance this localization has remained elusive. Here, we show that antiviral Tomato yellow leaf curl virus (TYLCV) genome requires body Nicotiana benthamiana cells. Methylation viral countered a virus-encoded protein, V2, which interacts with central component AGO4, interfering its binding DNA; V2-AGO4 interaction...
In plants, hybrid vigor is influenced by genetic and epigenetic mechanisms; however, the molecular pathways are poorly understood. We investigated potential contributions of regulators to heterosis in Arabidposis found that chromatin remodeler DECREASED DNA METHYLATION 1 (DDM1) affects early seedling growth Col/C24 hybrids. ddm1 mutants showed impaired increased expression non-additively expressed genes related salicylic acid metabolism. Interestingly, our data suggest a hormetic regulator...
Significance In plants, several DNA methylation pathways help maintain patterns to ensure that transposons remain in a silenced state and cell-specific is preserved after cell division. Here, we demonstrate loss of function the chromatin remodeler DDM1 induces pathway conversion from CMT2- RdDM-dependency maintenance CHH methylation. plants defective both RdDM, there strong reactivation TEs burst TE transposition. Thus, our work not only identifies variant RdDM pathway, but also shows at...
CRISPR/dCas9 is an important DNA modification tool in which a disarmed Cas9 protein with no nuclease activity fused specific modifying enzyme. A previous study reported that overexpression of the TET1 catalytic domain (TET1cd) reduces genome-wide methylation Arabidopsis. spontaneous naturally occurring region (NMR19-4) was identified promoter PPH (Pheophytin Pheophorbide Hydrolase) gene, encodes enzyme can degrade chlorophyll and accelerate leaf senescence. The status NMR19-4 associated...
The tea-oil tree Camellia oleifera is native to China and cultivated in many parts of southern China. This plant has been grown for over 2,000 years, mainly its high-quality cooking oil. Anthracnose the main disease results a huge loss annually. Colletotrichum fructicola major pathogen causing anthracnose on tree. In previous study, we characterized that bZIP transcription factor CfHac1 controlled development pathogenicity C. fructicola. Here, identified function CfVAM7 gene, which was...
In deep-sea geothermal rift zones, the dispersal of hydrothermal fluids moderately-high temperatures typically forms subseafloor mounds. Major mineral components crust covering mound are barite and metal sulfides. As a result continental rifting along Red Sea, metalliferous sediments accumulate on seafloor Atlantis II Deep. present study, was identified in sediment core from Deep, indicating formation at sampling site. Here, we examined how such dense could affect local environment...
Abstract In Arabidopsis , RNA‐directed DNA methylation (RdDM) is required for the maintenance of CHH methylation, and de novo in all (CG, CHG, CHH) contexts, but no obvious effect RdDM deficiency on plant development has been found to date. We show that combination mutations chromatin remodeler PKL components results developmental alterations, which appear a SUPPRESSOR OF DRM1 DRM2 CMT3 ( SDC )‐dependent manner.
ABSTRACT In plants, establishment of de novo DNA methylation is regulated by the RNA-directed (RdDM) pathway. RdDM machinery known to concentrate in Cajal body, but biological significance this localization has remained elusive. Here, we show that anti-viral Tomato yellow leaf curl virus (TYLCV) genome occurs specifically body Nicotiana benthamiana cells. Methylation viral countered a virus-encoded protein, V2, which interacts with central component AGO4, interfering its binding DNA; V2-AGO4...
Abstract Background Tea oil is widely used as edible in China, which extracted from the seeds of Camellia oleifera . In national oil-tea camellia planting area reached 4.533 million hectares, output seed was 627 000 tons, and total value 18.3 billion dollars. Anthracnose common disease Ca. , affected production brought huge economic losses. Colletotrichum fructicola dominant pathogen causing anthracnose oleifera. The retromer complex participates intracellular retrograde transport cargos...
Abstract Genetic variation affecting the binding of transcription factors (TFs) has been proposed as a major mechanism underlying susceptibility to common disease. NKX2-5, key cardiac development TF, associated with electrocardiographic (EKG) traits through GWAS, but extent which differential NKX2-5 contributes these not yet studied. Here, we analyzed transcriptomic and epigenomic data generated from iPSC-derived cardiomyocyte lines (iPSC-CMs) seven whole-genome sequenced individuals in...
Anthracnose, caused by
The ‘Cuimi’ kumquat is a unique citrus cultivar known for its thin, crisp pulp and sweet, aromatic flavor. In addition to use in fresh consumption processing, this variety exhibits certain medicinal properties. This study aims investigate the genetic diversity of Huanglongbing (HLB) bacterium across different tissues kumquat, offering theoretical basis understanding HLB epidemic Dechang County, Sichuan. research focuses on absolute quantification seven specific including new leaves, upper...