A5000298077- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Supramolecular Self-Assembly in Materials
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- French Urban and Social Studies
- Bacteriophages and microbial interactions
- Protein Kinase Regulation and GTPase Signaling
- S100 Proteins and Annexins
- Ubiquitin and proteasome pathways
- Erythrocyte Function and Pathophysiology
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Force Microscopy Techniques and Applications
- RNA regulation and disease
University of Warwick
2017-2025
The Francis Crick Institute
2021-2022
Clathrin-mediated endocytosis (CME) is the major route of receptor internalization at plasma membrane. Analysis constitutive CME difficult because initiation endocytic events unpredictable. When and where a clathrin-coated pit will form what cargo it contain are to foresee. Here we describe series genetically encoded reporters that allow on demand. A clathrin-binding protein fragment (“hook”) inducibly attached an “anchor” membrane, which triggers formation new vesicles. Our design...
The mitotic spindle is a bipolar array of microtubules, radiating from the poles which each contain centrosome, embedded in pericentriolar material. Two proteins, ch-TOG and TACC3, have multiple functions at due to operating either alone, together, or complex with other proteins. To distinguish these activities, we need new molecular tools dissect their function. Here, present structure α-helical bundle domain that mediates its interaction TACC3 structural model describing interaction,...
The formation of a clathrin-coated vesicle (CCV) is major membrane remodeling process that crucial for traffic in cells. Besides clathrin, these vesicles contain at least 100 different proteins although it unclear how many are essential the vesicle. Here, we show intracellular can be induced living cells using minimal machinery and achieved on various membranes, including mitochondrial outer membrane. Chemical heterodimerization was used to inducibly attach clathrin-binding fragment 'hook'...
Transport of proteins and lipids from one membrane compartment to another is via intracellular vesicles. We investigated the function tumor protein D54 (TPD54/TPD52L2) found that TPD54 was involved in multiple trafficking pathways: anterograde traffic, recycling, Golgi integrity. To understand how controls these diverse functions, we used an inducible method reroute mitochondria. Surprisingly, this manipulation resulted capture many small vesicles (30 nm diameter) at mitochondrial surface....
Tagging a protein of interest with GFP using genome editing is popular approach to study function in cell and developmental biology. To avoid re-engineering lines or organisms order introduce additional tags, functionalized nanobodies that bind can be used extend the functionality tag. We developed nanobodies, which we termed 'dongles', could add, for example, an FKBP tag GFP-tagged interest, enabling knocksideways experiments knock-in lines. The power it allows investigators rapidly switch...
Membrane traffic is an important regulator of cell migration through the endocytosis and recycling surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs) are transport vesicles that involved in multiple membrane trafficking steps, including pathway. The only known marker for INVs tumor protein D54 (TPD54/TPD52L2), a member TPD52-like family. Overexpression family proteins cancer has been linked to poor prognosis aggressive metastatic phenotype, which suggests may...
Article6 September 2021Open Access Transparent process Multi-modal adaptor-clathrin contacts drive coated vesicle assembly Sarah M Smith orcid.org/0000-0002-5349-1902 School of Life Sciences, University Warwick, Coventry, UK Search for more papers by this author Gabrielle Larocque orcid.org/0000-0001-8295-9378 Centre Mechanochemical Cell Biology, Warwick Medical School, Katherine Wood orcid.org/0000-0003-3723-097X Kyle L Morris orcid.org/0000-0002-1717-8134 Alan Roseman...
Transport of proteins and lipids from one membrane compartment to another is via intracellular vesicles. We investigated the function Tumor Protein D54 (TPD54/TPD52L2), found that TPD54 was involved in multiple trafficking pathways: anterograde traffic, recycling Golgi integrity. To understand how controls these diverse functions, we used an inducible method reroute mitochondria. Surprisingly, this manipulation resulted capture many small vesicles (30 nm diameter) at mitochondrial surface....
The formation of a clathrin-coated vesicle is major membrane remodeling process that crucial for traffic in cells. Besides clathrin, these vesicles contain at least 100 different proteins although it unclear how many are essential the vesicle. Here, we show intracellular can be induced living cells using minimal machinery and achieved on various membranes, including mitochondrial outer membrane. Chemical heterodimerization was used to inducibly attach clathrin-binding fragment "hook" an...
Tagging a protein-of-interest with GFP using genome editing is popular approach to study protein function in cell and developmental biology. To avoid re-engineering lines or organisms order introduce additional tags, functionalized nanobodies that bind can be used extend the functionality of tag. We developed nanobodies, which we termed “dongles”, could add, for example, an FKBP tag GFP-tagged protein-of-interest; enabling knocksideways experiments knock-in lines. The power it allows...
Membrane traffic is an important regulator of cell migration through the endocytosis and recycling surface receptors such as integrin heterodimers. Intracellular nanovesicles (INVs), are a recently identified class transport vesicle that involved in multiple membrane trafficking steps including pathway. The only known marker for INVs Tumor Protein D54 (TPD54/TPD52L2), member TPD52-like protein family. Overexpression family proteins cancer has been linked to poor prognosis aggressive...
Clathrin-coated pits are formed by the recognition of membrane and cargo heterotetrameric AP2 complex subsequent recruitment clathrin triskelia. A potential role for in coated-pit assembly beyond initial has not been explored. Clathrin binds β2 subunit AP2, several binding sites on heavy chain have identified, but our structural knowledge these interactions is incomplete their functional importance during endocytosis unclear. Here, we analysed cryo-EM structure cages assembled presence hinge...