A5089097891- Synthesis and biological activity
- RNA Interference and Gene Delivery
- Quinazolinone synthesis and applications
- Nanoparticle-Based Drug Delivery
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Cancer therapeutics and mechanisms
- Synthesis and Biological Evaluation
- Acute Myocardial Infarction Research
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac Imaging and Diagnostics
- Dendrimers and Hyperbranched Polymers
- Infective Endocarditis Diagnosis and Management
- Surfactants and Colloidal Systems
- Supramolecular Self-Assembly in Materials
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cardiac Valve Diseases and Treatments
- Coronary Interventions and Diagnostics
- Supramolecular Chemistry and Complexes
- Cardiac pacing and defibrillation studies
- Research on Leishmaniasis Studies
- Inflammatory Myopathies and Dermatomyositis
- Protein Degradation and Inhibitors
- Educational Leadership and Innovation
Universidad de Salamanca
2019-2024
Instituto de Investigación Biomédica de Salamanca
2019-2024
Evolved Analytics (United States)
2024
Public Health England
2021
Universidad de Sevilla
2012-2020
University of Castilla-La Mancha
2020
Instituto de Salud Carlos III
2020
Biomedical Research Networking Center on Neurodegenerative Diseases
2020
Rede de Química e Tecnologia
2014
Instituto de Investigaciones Químicas
2012
Importance In the Revascularization for Ischemic Ventricular Dysfunction (REVIVED-BCIS2) trial, percutaneous coronary intervention (PCI) did not improve outcomes patients with ischemic left ventricular dysfunction. Whether myocardial viability testing had prognostic utility these or identified a subpopulation who may benefit from PCI remained unclear. Objective To determine effect of extent viable and nonviable myocardium on effectiveness PCI, prognosis, improvement in function. Design,...
Ventricular arrhythmia is an important cause of mortality in patients with ischemic left ventricular dysfunction. Revascularization coronary artery bypass graft or percutaneous intervention often recommended for these before implantation a cardiac defibrillator because it assumed that this may reduce the incidence fatal and potentially arrhythmias, although premise has not been evaluated randomized trial to date.
Complete revascularization of coronary disease has been linked to improved outcomes in patients with preserved left ventricular (LV) function. To identify the impact complete severe LV dysfunction. Patients enrolled REVIVED-BCIS2 trial were eligible if baseline/procedural angiograms and viability studies available for analysis by independent core laboratories. Anatomical viability-guided completeness measured myocardial indices (RIcoro RImyo) respectively, where RIcoro=[change BCIS Jeopardy...
Multi-head/multi-tail facial amphiphiles built on cyclodextrin (CD) and calixarene (CA) scaffolds are paradigmatic examples of monodisperse gene delivery systems. The possibility to precisely control the architectural features at molecular level offers unprecedented opportunities for conducting structure-activity relationship studies. A major requirement those channels is design a sufficiently diverse ensemble compounds parallel evaluation their capabilities condense DNA into transfection...
Calixarene–cyclodextrin heterodimers self-assemble in water into core–shell nanoparticles that can be decorated with glycoligands for site-specific drug delivery.
Abstract Only a few examples of monodisperse molecular entities that can compact exogenous nucleic acids into nanocomplexes, protect the cargo from biological environment, facilitate cell internalization, and promote safe transfection have been reported up to date. Although these species open new venues for fundamental studies on structural requirements govern intervening processes their application in nonviral gene‐vector design, synthesis moieties generally requires relatively...
Giant amphiphiles encompassing a hydrophilic β-cyclodextrin (βCD) component and hydrophobic calix[4]arene (CA4) module undergo self-assembly in aqueous media to afford core-shell nanospheres or nanocapsules, depending on the nanoprecipitation protocol, with high docetaxel (DTX) loading capacity. The blank loaded nanoparticles have been fully characterized by dynamic light scattering (DLS), ζ-potential measurements cryo-transmission electron microscopy (cryo-TEM). data are compatible...
Abstract The ability of cyclodextrin‐based polycationic cluster to undergo reversible DNA condensation and release in a physiologically useful pH window has been finely tuned by the installation capping xylylene moiety at secondary face cyclooligosaccharide. This strategy can be exploited advantageously design self‐assembling nonviral gene‐delivery systems from molecular entities.
The design of colchicine site ligands on tubulin has proven to be a successful strategy develop potent antiproliferative drugs against cancer cells. However, the structural requirements binding endow with low aqueous solubility. In this work, benzothiazole scaffold is used design, synthesize, and evaluate new family exhibiting high water compounds exerted activity several human cell lines, due polymerization inhibition, showing selectivity toward cells in comparison non-tumoral HEK-293...
In the Revascularization for Ischemic Ventricular Dysfunction (REVIVED-BCIS2) trial, percutaneous coronary intervention (PCI) did not reduce incidence of death or hospitalization heart failure. This prespecified secondary analysis investigated effect PCI on health status measured with KCCQ combined primary outcome in a win ratio. Participants severe ischemic left ventricular dysfunction were randomized to either addition optimal medical therapy alone (OMT). The was hierarchical composite...
Tubulin is a well-validated target for herbicides, fungicides, anti-parasitic, and anti-tumor drugs. Many of the non-cancer tubulin drugs bind to its colchicine site but no colchicine-site anticancer drug available. The composed three interconnected sub-pockets that fit their ligands modify others' preference, making design molecular hybrids (that more than one sub-pocket) difficult task. Taking advantage eighty published X-ray structures in complex with bound site, we generated an ensemble...
Engineering self-assembled superstructures through complexation of plasmid DNA (pDNA) and single-isomer nanometric size macromolecules (molecular nanoparticles) is a promising strategy for gene delivery. Notably, the functionality overall architecture vector can be precisely molded at atomic level by chemical tailoring, thereby enabling unprecedented opportunities structure/self-assembling/pDNA delivery relationship studies. Beyond this notion, judiciously preorganizing functional elements...
Abstract We designed a set of multi‐galactosides with valencies ranging from one to seven and different spacer‐arm lengths. The compounds display high structural homology for strict assessment multivalent phenomena. multimers were first evaluated by an enzyme‐linked lectin assay (ELLA) toward the peanut agglutinin (PNA). binding affinity was shown be dependent on length, cluster effects observed galactosides bearing shortest longest linkers. latter much more potent PNA cross‐linkers in...
Colchicine site ligands suffer from low aqueous solubility due to the highly hydrophobic nature of binding site. A new strategy for increasing molecular polarity without exposing polar groups-termed masked group incorporation (MPGI)-was devised and applied nitrogenated combretastatin analogues. Bulky ortho substituents pyridine nitrogen hinder it pocket while polarity. The resulting analogues show improved solubilities potent antiproliferative activity against several cancer cell lines...
To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector.siRNA protection from degradation by RNAses, efficiency and thermodynamic parameters of nanoparticle/siRNA interactions were studied on pairs amphiphilic molecules using biochemical techniques molecular dynamics.The lower solvent accessible surface area in presence nanoparticle, higher RNAse-mediated corresponding nanocomplex; moderate binding energy...
Increasing awareness of the structure microtubules has made tubulin a relevant target for research novel chemotherapies. Furthermore, particularly high sensitivity glioblastoma multiforme (GBM) cells to microtubule disruption could open new doors in search anti-GBM treatments. However, difficulties developing potent anti-tubulin drugs endowed with improved pharmacokinetic properties necessitates expansion medicinal chemistry campaigns. The application an ensemble pharmacophore screening...
The low aqueous solubility of colchicine site antimitotic agents, which the trimethoxyphenyl (A ring) is a heavy contributor, serious drawback in their clinical development. We have designed new A ring analogs with chameleonic masked polar amino groups able to increase and also behave as non-polar through intramolecular hydrogen bonds when bound tubulin. incorporated these rings several scaffolds (sulfonamides, combretastatins, phenstatins, isocombretastatins), synthesized, assayed 43...