A5101730205- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Immune Response and Inflammation
- Spaceflight effects on biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Inflammatory Bowel Disease
- Corneal Surgery and Treatments
- RNA modifications and cancer
- Proteoglycans and glycosaminoglycans research
- Tryptophan and brain disorders
- Facial Nerve Paralysis Treatment and Research
- Heat shock proteins research
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Corneal surgery and disorders
- Trace Elements in Health
- Amyotrophic Lateral Sclerosis Research
- Epigenetics and DNA Methylation
- Prion Diseases and Protein Misfolding
- Cytokine Signaling Pathways and Interactions
Nantong University
2014-2025
China Astronaut Research and Training Center
2013-2024
Army Medical University
2024
Shanxi Medical University
2023-2024
New York State Office for People With Developmental Disabilities
2017-2024
Soochow University
2012-2018
Air Force Institute of Aviation Medicine Affiliated Hospital
2018
University of Chicago
2010-2016
The University of Texas Southwestern Medical Center
2016
Southwestern Medical Center
2016
Lumican and fibromodulin regulate the assembly of collagens into higher order fibrils in connective tissues. Here, we show that mice deficient both these proteoglycans manifest several clinical features Ehlers-Danlos syndrome. TheLum −/− Fmod are smaller than their wild type littermates display gait abnormality, joint laxity, age-dependent osteoarthritis. Misaligned knee patella, severe dysmorphogenesis, extreme tendon weakness likely causes for laxity double-nulls. Fibromodulin deficiency...
Colonic microbiota ferment non-absorbed dietary fiber to produce prodigious amounts of short chain fatty acids (SCFAs) that benefit the host through a myriad metabolic, trophic, and chemopreventative effects. The effects SCFA butyrate are, in part, mediated induction p21 gene expression. In this study, we assessed role microRNA(miRNA) butyrate's expression profiles miRNAs HCT-116 cells human sporadic colon cancers were by microarray quantitative PCR. Regulation miR-106b was 3' UTR luciferase...
Abstract Epithelial–mesenchymal transition (EMT) promotes cancer invasion and metastasis, but the integrative mechanisms that coordinate these processes are incompletely understood. In this study, we used a cross-species expression profiling strategy in metastatic cell lines of human mouse origin to identify 22 up-regulated 12 down-regulated genes part an essential genetic program metastasis. particular, identified novel function metastasis was not previously known for transcription factor...
Fibrosis is a major complication of chronic inflammation, as seen in Crohn's disease and ulcerative colitis, two forms inflammatory bowel diseases. To elucidate signals that regulate fibrosis, we investigated gene expression changes underlying inflammation fibrosis trinitrobenzene sulfonic acid-induced murine colitis. Six weekly 2,4,6-trinitrobenzene acid enemas were given to establish colitis temporal patterns obtained at 6-, 8-, 10-, 12-wk time points. The 6-wk point, TNBS-w6, was the...
BackgroundCrohn's disease (CD) is associated with defective sensing of pathogens in genetically susceptible individuals. Nucleotide-binding oligomerization domain containing 2 (NOD2) mutations coding regions are strongly linked to CD pathogenesis. Our laboratory has reported that microRNAs (miRNAs) differentially expressed CD. However, miRNA regulation NOD2 remains unknown. This study was designed determine whether miRNAs regulate expression as well downstream nuclear factor kappaB...
Dysfunction of the autophagy-lysosomal pathway (ALP) and ubiquitin-proteasome system (UPS) was thought to be an important pathogenic mechanism in synuclein pathology Parkinson's disease (PD). In present study, we investigated role sestrin2 autophagic degradation α-synuclein preservation cell viability a rotenone-induced cellular model PD. We speculated that AMP-activated protein kinase (AMPK) involved regulation autophagy protection dopaminergic cells against rotenone toxicity by sestrin2....
Poloxamer 188 (P188), a multiblock copolymer surfactant, has been shown to protect against ischemic tissue injury of cardiac muscle, testes and skeletal but the mechanisms have not fully understood. In this study, we explored whether P188 had protective effect cerebral ischemia/reperfusion its underlying mechanisms. The in vivo results showed that significantly reduced infarct volume, ameliorated brain edema neurological symptoms 24 h after ischemia/reperfusion. long-term outcome markedly...
Depression is a neuropsychiatric disorder accompanied by decrease in the brain-derived neurotrophic factor (BDNF) signalling cascade hippocampus. Fenofibrate selective agonist of PPAR-α. In this study, we investigated antidepressant-like effects fenofibrate C57BL/6J mice.The were first identified forced swim test (FST) and tail suspension (TST), then assessed chronic social defeat stress (CSDS) model. The changes hippocampal BDNF pathway adult neurogenesis after CSDS treatment further...
Long noncoding RNAs (lncRNAs) were recently found to be key regulators of biological functions and associated with human diseases. Thus far, the roles lncRNAs in inflammatory bowel disease (IBD) remain unknown. We aimed determine whether are IBD regulate epithelial cell physiology.lncRNAs microarray quantitative RT-PCR performed on 60 sigmoid colon biopsies from patients active ulcerative colitis (UC) relevant controls. Localization was detected by situ hybridization subcellular RNA. The...
Multiple genetic studies have implicated the autophagy-related gene, ATG16L1, in pathogenesis of Crohn disease (CD). While CD-related research on ATG16L1 has focused functional significance variations, mechanisms underlying regulation expression are unclear. Our laboratory described that microRNAs (miRNAs), key regulators gene expression, dysregulated CD. Here, we report miRNA-mediated colonic epithelial cells as well Jurkat T cells. Dual luciferase reporter assays following transfection...
Abstract HSP70, a stress response protein, is known to be determinant of cell death and transformation. We show that different isoforms p63 have transcriptional activities on hsp70 genes. ΔNp63α, an abundantly expressed isoform p63, activates (in vitro in vivo), whereas TAp63γ down-regulates the expression hsp70. further transactivation domain at NH2 terminus represses, COOH transcription. In addition, ΔNp63α regulates transcription gene through its interaction with CCAAT binding factor NF-Y...
Gait deficits are important clinical symptoms of Parkinson's disease (PD). However, existing behavioral tests for the detection motor impairments in rodents with systemic dopamine depletion only measure akinesia and dyskinesia, data focusing on gait scarce. We evaluated changes methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced C57BL/6 murine model PD by using a computer-assisted CatWalk system. Correlations parameters tyrosine hydroxylase (TH) protein levels substantia nigra (SN)...
Abnormal innate immune response contributes to inflammatory bowel disease (IBD) and experimental mouse colitis. Colitis studies have focused primarily on key regulators of immunity, like pathogen recognition receptors cytoplasmic mediators. Extracellular matrix (ECM) proteins are emerging as modulators responses by virtue their interactions with pathogen-associated molecular patterns (PAMPs), cytokines, growth factors, receptors, ECM fragments that mimic pathogens or cytokines. The not been...
The microRNAs (miRNAs) regulate gene expression at the posttranscriptional level. ATG16L1, an essential component for autophagy and a risk Crohn's disease, contains two binding sites in 3'UTR miR-17 family, including miRs-20a, -93, -106a, -106b. purpose of this study was to assess effects these miRNAs on ATG16L1 autophagic activity HCT116 cells.The functional were evaluated by transfection pMIR-GLO vectors bearing wild type or mutant into cells luciferase reporter assay. miRNA regulation...
In the brains of individuals with Alzheimer's disease (AD) and chronic traumatic encephalopathy, tau pathology is accompanied usually by intracellular aggregation transactive response DNA-binding protein 43 (TDP-43). However, role TDP-43 in pathogenesis not understood. Here, we investigated expression vitro vivo. We found that suppressed promoting its mRNA instability through UG repeats 3΄-untranslated region (3΄-UTR). The C-terminal was required for this function. Neurodegenerative...
Background and Purpose Developing novel pharmacological targets beyond the monoaminergic system is now a popular strategy for treating depression. PPARα nuclear receptor protein that functions as transcription factor,‐regulating gene expression. We have previously reported both WY14643 fenofibrate, two agonists of PPARα, antidepressant‐like effects in mice, implying potential antidepressant target. Experimental Approach first used various biotechnological methods to evaluate chronic stress...
Abstract The formation of differentiated cell types from pluripotent progenitors involves epigenetic regulation gene expression. DNA hydroxymethylation results the enzymatic oxidation 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) by ten-eleven translocation (TET) 5-mC dioxygenase enzymes. Previous work has mapped changes in during differentiation intestinal stem cells. However, whether or not 5-hmC regulates colonocyte is unknown. Here we show that expression and controls human...