A5076679983- FOXO transcription factor regulation
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Bacterial biofilms and quorum sensing
- Cardiovascular Disease and Adiposity
- Molecular Biology Techniques and Applications
- Renal cell carcinoma treatment
- Cardiovascular Function and Risk Factors
- Cytomegalovirus and herpesvirus research
- PI3K/AKT/mTOR signaling in cancer
- Prostate Cancer Treatment and Research
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Ubiquitin and proteasome pathways
- Antibiotic Resistance in Bacteria
- Nutrition, Genetics, and Disease
- Peptidase Inhibition and Analysis
- Viral Infections and Vectors
- Respiratory viral infections research
- Digestive system and related health
Central South University
2013-2025
Xi’an Children’s Hospital
2024
Northwest University
2023-2024
Cincinnati Children's Hospital Medical Center
2021-2023
The Barbara Ann Karmanos Cancer Institute
2012-2022
Wayne State University
2013-2022
Air Force Medical University
2005-2020
Xijing Hospital
2005-2020
Wuhan University of Science and Technology
2020
Huaibei Normal University
2010-2011
Background: The threat of drug-resistant Pseudomonas aeruginosa requires great efforts to develop highly effective and safe bactericide. Objective: This study aimed investigate the antibacterial activity mechanism silver nanoparticles (AgNPs) against multidrug-resistant P. . Methods: antimicrobial effect AgNPs on clinical isolates resistant P was assessed by minimal inhibitory concentration (MIC) bactericidal (MBC). In , alterations morphology structure were observed transmission electron...
Abstract Introduction Phosphatidylinositol 3-kinases (PI3Ks) are a group of lipid kinases that regulate signaling pathways involved in cell proliferation, adhesion, survival, and motility. Even though PIK3CA amplification somatic mutation have been reported previously various kinds human cancers, the genetic change breast cancer has not clearly identified. Methods Fifteen lines 92 primary tumors (33 with matched normal tissue) were used to check gene copy number . For study, we specifically...
Mitochondrial genomic mutations are found in a variety of human cancers; however, the frequency mitochondrial DNA (mtDNA) coding regions remains poorly defined, and functional effects primary cancers not well described. Using MitoChip, we sequenced whole genome 83 head neck squamous cell carcinomas. Forty-one (49%) tumors contained mtDNA mutations. Mutations occurred within noncoding (D-loop) regions. A nonrandom distribution was throughout enzyme complex components. Sequencing margins with...
As in many other human cancers, overactivation of the phosphotidylinositol 3-kinase (PI3K)/Akt signaling pathway occurs frequently thyroid cancer, but mechanism is not completely clear.Because activating mutations and genomic amplification PIK3CA gene, which encodes p110a catalytic subunit PI3K, are common we sought to investigate this phenomenon tumors.To search for mutations, isolated DNA from primary tumors various types performed direct sequencing exons gene that carry most cancers. We...
Abstract Epithelial–mesenchymal transition (EMT) promotes cancer invasion and metastasis, but the integrative mechanisms that coordinate these processes are incompletely understood. In this study, we used a cross-species expression profiling strategy in metastatic cell lines of human mouse origin to identify 22 up-regulated 12 down-regulated genes part an essential genetic program metastasis. particular, identified novel function metastasis was not previously known for transcription factor...
p63 is a member of the p53 tumor suppressor gene family, which regulates downstream target expression by binding to sequence-specific response elements similar those p53. By using oligonucleotide microarray analysis and analyzing promoters p63-induced genes, we have identified novel p63-specific (p63-REs) in promoter regions EVPL SMARCD3. These p63-REs exhibit characteristic differences from canonical p53-RE (RRRCWWGYYY) both core-binding element (CWWG) as well RRR and/or YYY stretches....
Context: The role of the T1799A BRAF mutation in lymph node metastasis papillary thyroid cancer (PTC) is not clear. Objective: Our objective was to explore relationship between and PTC by examining both primary tumors their paired metastases. Design: We isolated genomic DNA from metastases performed direct sequencing exon 15 gene that carries mutation. Results: In a series 33 cases, 21 harbored tumors, 17 (81%) them same also Twelve cases did harbor among which nine node-metastasized whereas...
Promoter hypermethylation is one of the common mechanisms leading to gene silencing in various human cancers. Using a combination pharmacologic unmasking and microarray techniques, we identified 59 candidate hypermethylated genes, including LOXL1, lysyl oxidase-like gene, bladder cancer cells. We further showed that LOXL1 LOXL4 are commonly silenced genes cells, this silence predominantly related promoter methylation. also found methylation loss expression primary tumors. In addition,...
Frequent genetic alterations of the components in phosphoinositide 3-kinase (PI3K)/PTEN/AKT signaling pathway contribute greatly to breast cancer initiation and progression, which makes targeting this a promising therapeutic strategy for treatment. In study, we showed that presence copper (Cu), disulfiram (DSF), clinically used antialcoholism drug, could potently inhibit cell growth regardless PIK3CA status. Surprisingly, treatment with mixture DSF (DSF-Cu) led decreased expression PTEN...
In this study, we have showed that GCNT2, a gene-encoding glucosaminyl (N-acetyl) transferase 2, I-branching enzyme, is overexpressed in highly metastatic breast cancer cell lines of human and mouse origin basal-like tumor samples. GCNT2 expression also significantly correlated to the phenotype Functional studies ectopic enhances detachment, adhesion endothelial cells, migration invasion vitro, lung metastasis cells vivo. Knockdown decreases vitro We further shown involvement...
Many epithelial-mesenchymal transition (EMT)-promoting transcription factors have been implicated in tumorigenesis and metastasis as well chemoresistance of cancer. However, the underlying mechanisms mediating these processes are unclear. Here, we report that Foxq1, a forkhead box-containing factor EMT-inducing gene, promotes stemness traits mammary epithelial cells. Using an expression profiling assay, identified Twist1, Zeb2, PDGFRα β Foxq1 downstream targets. We further show can be...
The decline of clinically effective antibiotics has made it necessary to develop more antimicrobial agents, especially for refractory biofilm-related infections. Silver nanoparticles (AgNPs) are a new type agent that can eradicate biofilms and reduce bacterial resistance, but its anti-biofilm mechanism not been elucidated. In this study, we investigated the molecular AgNPs against multidrug-resistant Pseudomonas aeruginosa by means tests, scanning electron microscopy (SEM), tandem mass tag...
Background: The intestinal flora can influence behavior through the microbiota-gut-brain axis and is closely related to occurrence development of nervous system diseases such as depression. Probiotics like Lactobacillus may regulate balance play an active role in preventing treating Methods: Eight-week-old C57BL/6J mice (n=32) were randomly equally divided into a normal control group, + Lac model group. groups intraperitoneally injected with 1.2 mg/kg lipopolysaccharide for 7 days, was...
Abstract HSP70, a stress response protein, is known to be determinant of cell death and transformation. We show that different isoforms p63 have transcriptional activities on hsp70 genes. ΔNp63α, an abundantly expressed isoform p63, activates (in vitro in vivo), whereas TAp63γ down-regulates the expression hsp70. further transactivation domain at NH2 terminus represses, COOH transcription. In addition, ΔNp63α regulates transcription gene through its interaction with CCAAT binding factor NF-Y...
Prostate cancer is the most common in adult men North America. Preclinical studies of prostate employ genetically engineered mouse models, because does not occur naturally rodents. Widespread application these models has been limited autopsy was only reliable method to evaluate treatment efficacy longitudinal studies. This article reports first use three-dimensional ultrasound microimaging for measuring tumor progression a model, 94-amino acid secretory protein gene-directed transgenic...
Abstract We investigated the genotype-dependent therapeutic potential of targeting phosphoinositide 3-kinase (PI3K)/Akt pathway for thyroid cancer. Proliferation TPC1, Hth7, FTC133, OCUT1, K1, and BCPAP cells that harbored PI3K/Akt-activating genetic alterations was potently inhibited by Akt inhibitor perifosine, whereas SW1736, Hth74, WRO, KAT18, TAD2 no had or only modest responses. Inhibition phosphorylation perifosine seen in these cells. Genetic-dependent apoptosis induced selectively...
Background/Aims: The previous study in our lab showed that Nodal molecule on bronchial epithelial cells (BECs) was modulated by all kinds of lung microbes. present designed to determine the effects proliferation BECs and BECs-induced differentiation T-helper (Th) cells. epigenetic mechanisms expression following treatments different microbes were also identified. Methods: Real-time polymerization chain reaction (PCR) western blot used Nodal. Flow cytometry observe subsequent Th Methylation...