A5010450770- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Ion Transport and Channel Regulation
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- Renal and related cancers
- RNA modifications and cancer
- Heat shock proteins research
- Bladder and Urothelial Cancer Treatments
- Cancer Research and Treatments
- Genetic factors in colorectal cancer
- Fibroblast Growth Factor Research
- Mitochondrial Function and Pathology
- Colorectal Cancer Screening and Detection
- Peptidase Inhibition and Analysis
- Trypanosoma species research and implications
- Neonatal Respiratory Health Research
- Ion channel regulation and function
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Xenotransplantation and immune response
- Toxin Mechanisms and Immunotoxins
- Axon Guidance and Neuronal Signaling
Johns Hopkins University
2006-2023
Cancer Research Foundation
2021-2023
Clinical Research Services
2021
Johns Hopkins Medicine
1993-2019
Sidney Kimmel Comprehensive Cancer Center
2003-2015
Craig Hospital
2010
Asan Medical Center
2007-2008
Palmetto Hematology Oncology
2005-2008
Einstein Medical Center Philadelphia
2008
Harvard University
2008
Abstract Purpose: To evaluate aberrant promoter hypermethylation of candidate tumor suppressor genes as a means to detect epigenetic alterations specific solid tumors, including head and neck squamous cell carcinoma (HNSCC). Experimental Design: Using regions identified via gene discovery approach, we evaluated the ability an expanded panel CpG-rich promoters known be differentially hypermethylated in HNSCC detection serum salivary rinses associated with HNSCC. We did preliminary evaluation...
The aquaporins (AQP) are water channel proteins playing a major role in transcellular and transepithelial movement. Recently, the of AQPs human carcinogenesis has become an area great interest. Here, by immunohistochemistry (IHC), we have found expression AQP5 protein 35.3% (IHC-score: ≥1, 144/408) resected NSCLC tissue samples. Cases with AQP5-positive status ≥2) displayed higher rate tumor recurrence than negative ones (54.7% vs. 35.1%, p = 0.005) worse disease-free survival (p 0.033; OR...
Aberrant promoter hypermethylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis lung cancers and is a promising marker for cancer detection. We investigated feasibility detecting aberrant DNA methylation in bronchoalveolar lavage (BAL) samples patients.We examined matched BAL eight gene promoters (CDH1, APC, MGMT, RASSF1A, GSTP1, p16, RAR-beta 2, ARF) from 31 patients with primary tumors by quantitative fluorogenic real-time PCR. 10...
Abstract Aquaporin-CHIP is the first known molecular water channel. Originally identified in red cells and renal tubules, transcripts proteins related to AQP-CHIP are also expressed diverse epithelia with distinct developmental patterns. Northern analyses of RNA from several tissues revealed 3.1 kilobases other sizes. The nucleotide sequences human kidney cDNAs identical bone marrow cDNA. 17-kilobase structural gene was isolated, restriction maps were constructed partially sequenced. TATA...
The aquaporin-1 (AQP1) water channel protein is expressed in multiple mammalian tissues by several different developmental programs; however, the genetic regulation undefined. proximal promoter of mouse Aqp1 contains putative cis-acting regulatory elements, and erythroleukemia (MEL) cells are a well-characterized model for erythroid differentiation. Corticosteroid or dimethyl sulfoxide (DMSO) exposure induces AQP1 expression MEL cells, transcriptional was investigated transient transfections...
Abstract Promoter hypermethylation accompanied by gene silencing is a common feature of human cancers. We identified previously several new tumor suppressor genes based on pharmacologic unmasking the promoter region and detection reexpression microarray analysis. In this study, we modified selection candidates from our previous data excluding that showed basal expression in cancer cell lines. With method, found novel methylated with 90% accuracy. Among these 33 esophageal squamous carcinoma...
Abstract HSP70, a stress response protein, is known to be determinant of cell death and transformation. We show that different isoforms p63 have transcriptional activities on hsp70 genes. ΔNp63α, an abundantly expressed isoform p63, activates (in vitro in vivo), whereas TAp63γ down-regulates the expression hsp70. further transactivation domain at NH2 terminus represses, COOH transcription. In addition, ΔNp63α regulates transcription gene through its interaction with CCAAT binding factor NF-Y...
Aquaporins (AQPs) have previously been associated with increased expression in solid tumors. However, its hematologic malignancies including CML has not described yet. Here, we report the of AQP5 cells by RT-PCR and immunohistochemistry. While normal bone marrow biopsy samples (n = 5) showed no AQP5, 32% patient 41) demonstrated expression. In addition, level emergence imatinib mesylate resistance paired (p 0.047). We found that overexpression K562 resulted cell proliferation. small...
Aquaporins (AQPs) are molecular water channels, and 10 related AQPs have been identified in mammals. So far, the study of mammalian AQP expression has limited mainly to mice rat lung, kidney, brain, gastrointestinal tract. Although AQP3 AQP7 shown be involved volume-regulating mechanisms dendritic cells, exact patterns human immune system not well understood. Using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis peripheral blood from healthy donors, we demonstrated AQP1,...
Abstract Based on the oncogenic role of phosphatidylinositol glycan (PIG) class U in human tumors, we explored two additional subunits glycosylphosphatidylinositol (GPI) transamidase complex breast cancer. We found that PIG T (PIG-T) and GPI anchor attachment 1 (GPAA1) were overexpressed cancer cell lines primary tumors. Forced expression PIG-T GPAA1 transformed NIH3T3 cells vitro increased tumorigenicity invasion these vivo. Suppression led to inhibition anchorage-independent growth....
NMDA receptor Type 2B (NMDAR2B) is a candidate TSG first identified in esophageal squamous cell carcinoma (ESCC). To evaluate NMDAR2B methylation gastric cancer progression, we performed quantitative methylation-specific PCR (MSP), RT-PCR and immnunohistochemistry (IHC) primary tissues colony formation assays lines. We found that the expression of was reactivated by demethylating agent, 5-aza-2'-deoxycytidine, with or without trichostatin A Moreover, inactivation to be closely correlated...
Abstract Deleted in colorectal cancer (DCC) is a candidate tumor-suppressor gene located at chromosome 18q21. However, DCC was found to have few somatic mutations and the heterozygous mice (DCC+/−) showed similar frequency of tumor formation compared with wild-type (DCC+/+). Recently, came back spotlight as better understating its function relationship ligand (netrin-1) had shown that may act conditional gene. We evaluated hypermethylation mechanism for inactivation head neck squamous cell...