A5102839293- RNA modifications and cancer
- Head and Neck Cancer Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Ear and Head Tumors
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Oral and Maxillofacial Pathology
- Head and Neck Surgical Oncology
- Tumors and Oncological Cases
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Cervical Cancer and HPV Research
- Cancer and Skin Lesions
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Plant Disease Resistance and Genetics
- Medical Imaging and Pathology Studies
- Hedgehog Signaling Pathway Studies
- Thyroid Cancer Diagnosis and Treatment
Icahn School of Medicine at Mount Sinai
2018-2025
Mount Sinai Hospital
2018-2024
Albert Einstein College of Medicine
2023
Johns Hopkins University
2009-2018
Johns Hopkins Medicine
2004-2018
University of Baltimore
2000-2017
Johns Hopkins Hospital
1998-2016
National Institute of Dental and Craniofacial Research
2016
National Institutes of Health
2016
University of Virginia
2016
Human papillomavirus-associated head and neck squamous cell carcinomas (HPV-HNSCC) originate in the tonsils, major lymphoid organ that orchestrates immunity to oral infections. Despite its location, virus escapes immune elimination during malignant transformation progression. Here, we provide evidence for role of PD-1:PD-L1 pathway HPV-HNSCC resistance. We show membranous expression PD-L1 tonsillar crypts, site initial HPV infection. In HPV-HNSCCs are highly infiltrated with lymphocytes, on...
We and others recently isolated a human p53 homologue ( p40 / p51 p63 p73L ) localized the gene to distal long arm of chromosome 3. Here we sought examine role , two variants lacking N-terminal transactivation domain, in cancer. Fluorescent situ hybridization (FISH) analysis revealed frequent amplification this locus primary squamous cell carcinoma lung head neck cancer lines. (We named AIS for mplified i n s quamous carcinoma.) Furthermore, was accompanied by RNA protein overexpression...
The distinction of solitary fibrous tumors (SFTs) from histologically similar neoplasms relies heavily on a characteristic microscopic appearance. No discriminating ultrastructural or immunohistochemical features are known. We evaluated 22 SFTs and 118 other often considered in the differential diagnosis for immunoreactivity using monoclonal antibody directed against human hematopoietic progenitor cell antigen, CD34. All (22 22, 100%) demonstrated strong CD34 irrespective tumor site...
High-risk human papillomavirus (HPV) is an established cause of head and neck carcinomas arising in the oropharynx. The presence HPV has also been reported some sinonasal tract, but little known about their overall incidence or clinicopathologic profile. surgical pathology archives Johns Hopkins Hospital were searched for all tract from 1995 to 2011, tissue microarrays constructed. p16 immunohistochemical analysis DNA situ hybridization high-risk types performed. Demographic clinical outcome...
// Rafael Guerrero-Preston 1, 2 , Filipa Godoy-Vitorino 3 Anne Jedlicka 4 Arnold Rodríguez-Hilario Herminio González Jessica Bondy 1 Fahcina Lawson Oluwasina Folawiyo Christina Michailidi Amanda Dziedzic Rajagowthamee Thangavel 5 Tal Hadar Maartje G. Noordhuis 6 William Westra 7 Wayne Koch David Sidransky Department of Otolaryngology and Head Neck Surgery, Johns Hopkins University School Medicine, Baltimore, Maryland, USA Obstetrics Gynecology, Puerto Rico San Juan, Natural...
To more fully characterize the clinical and pathologic spectrum of a recently described tumor entity sinonasal tract characterized by loss nuclear expression SMARCB1 (INI1), we analyzed 39 SMARCB1-deficient carcinomas collected from multiple medical centers. The tumors affected 23 males 16 females with an age range 19 to 89 years (median, 52). All patients presented locally advanced disease (T3, n=5; T4, n=27) involving sinuses (mainly ethmoid) variable involvement nasal cavity. Thirty...
Human papillomavirus (HPV)–related multiphenotypic sinonasal carcinoma (HMSC), originally known as HPV-related with adenoid cystic carcinoma-like features, is a peculiar neoplasm that restricted to the tract, exhibits features of both surface-derived and salivary gland (particularly carcinoma), associated high-risk HPV. Given limited number published cases, full clinicopathologic spectrum this unclear. Here, we present an updated experience 49 cases. All cases HMSC were obtained from...
Abstract Purpose: Telomeres help maintain chromosomal integrity. Dysfunctional telomeres can cause genetic instability in vitro and an increased cancer incidence telomerase knock out mouse models. We recently reported that telomere shortening was a prevalent alteration human prostate, pancreas, breast precursor lesions. In the present study, we address whether previous findings are broadly applicable to epithelial precursors general. Experimental Design: Surgical specimens of lesions from...
When patients are referred to one's own institution for therapy based on a histopathologic diagnosis rendered at another institution, many hospitals require second opinion of the surgical pathology material. This quality assurance practice has been threatened in era managed care and cost containment.The authors reviewed impact mandatory The Johns Hopkins Hospital. Cases were collected prospectively over 21-month period from April 1995 December 1996. For purposes this study, changed was...
MicroRNAs (mirs) are small noncoding RNA molecules (~22 nucleotides) that regulate posttranscriptional gene expression. Currently, there has not been a comprehensive study of their role in primary head and neck squamous cell carcinoma (HNSCC). To determine the mirs HNSCC, we screened for altered microRNA expression HNSCC tissue lines. We then further tested functional impact alterations specific mirs. An initial screening 4 normal mucosal controls lines was analyzed mature by microarray....
The p53 gene (also known as TP53) may be the most common genetic target involved in malignant transformation of human cells. Direct sequence analysis has demonstrated that alteration this occurs approximately 45% head and neck squamous cell carcinomas. consequences mutations these cancers with respect to tumor behavior patient survival have not been rigorously determined.We evaluated implications relation control locoregional disease overall following radiation therapy.Data from 110...
Abstract To identify the molecular mechanisms by which EBV-associated epithelial cancers are maintained, we measured expression of essentially all human genes and latent EBV in a collection 31 laser-captured, microdissected nasopharyngeal carcinoma (NPC) tissue samples 10 normal tissues. Global gene profiles clearly distinguished tumors from healthy epithelium. Expression levels six viral (EBNA1, EBNA2, EBNA3A, EBNA3B, LMP1, LMP2A) were correlated among themselves strongly inversely with...
Abstract Purpose: Advances in targeted lung cancer therapy now demand accurate classification of non–small cell (NSCLC). MicroRNAs (miRNA) are recently discovered short, noncoding genes that play essential roles tissue differentiation during normal development and tumorigenesis. For example, hsa-miR-205 is a miRNA highly expressed squamous carcinomas (SqCC) but not adenocarcinomas. The differential expression miRNAs could be exploited to distinguish these tumor types. Experimental Design:...
Mitochondrial genomic mutations are found in a variety of human cancers; however, the frequency mitochondrial DNA (mtDNA) coding regions remains poorly defined, and functional effects primary cancers not well described. Using MitoChip, we sequenced whole genome 83 head neck squamous cell carcinomas. Forty-one (49%) tumors contained mtDNA mutations. Mutations occurred within noncoding (D-loop) regions. A nonrandom distribution was throughout enzyme complex components. Sequencing margins with...
Abstract NOTCH1 mutations have been reported to occur in 10% 15% of head and neck squamous cell carcinomas (HNSCC). To determine the significance these mutations, we embarked upon a comprehensive study NOTCH signaling cohort 44 HNSCC tumors 25 normal mucosal samples through set expression, copy number, methylation, mutation analyses. Copy number increases were identified pathway genes, including ligand JAG1. Gene analysis defined differential expression relative tissues. Analysis individual...
Background. Point mutations in codon 12 of the K-ras protooncogene occur more frequently lung adenocarcinomas from smokers (30%) than they do nonsmokers (7%), suggesting that smoking is an important factor induction these mutations. The lack well defined “early” premalignant or situ glandular neoplasms lung, however, has not permitted direct evaluation chronology ras activation development adenocarcinomas. To circumvent need to evaluate precursor lesions, we examined former for point...
SMARCB1 (INI-1) is a tumor-suppressor gene located on chromosome 22q11.2. Its product ubiquitously expressed in nuclei of all normal tissues. inactivation has been implicated the pathogenesis diverse group malignant neoplasms that tend to share "rhabdoid" cytomorphology. This SMARCB1-deficient tumors now further expanded by subset carcinomas arising sinonasal tract. immunostaining was performed 142 carcinomas. Tumors showed loss expression were characterized for deletions fluorescence situ...
Acinic cell carcinoma (ACC) is a low-grade salivary gland malignancy characterized by serous acinar differentiation. Most ACCs arise in the parotid gland, but have been reported to originate nonparotid glands where acini are less abundant. Given recent discovery of mammary analog secretory (MASC)-a that histologically mimics ACC-a retrospective reevaluation warranted. The surgical pathology archives Johns Hopkins Hospital were searched for all arising outside gland. For each case, histologic...
Human papillomavirus (HPV)-related carcinomas of the head and neck are characterized by a predilection for oropharynx, nonkeratinizing squamous morphology, infection with HPV 16 type; but comprehensive testing across all sites has shown that pathologic features HPV-related carcinoma may be more wide ranging than initially anticipated. In particular, subset sinonasal positive, these include variant is histologically similar to adenoid cystic (ACC). Cases were identified retrospective...
Despite the importance of recognizing neuroendocrine differentiation when diagnosing tumors thoracic cavity, sensitivity traditional markers is suboptimal, particularly for high-grade carcinomas such as small cell lung carcinoma and large carcinoma. To increase sensitivity, are routinely ordered panels multiple immunostains where any single positive marker regarded sufficient evidence differentiation. Insulinoma-associated protein 1 (INSM1) a well-validated transcription factor that has only...
Abstract DNA methylation has a role in mediating epigenetic silencing of CpG island genes cancer and other diseases. Identification all gene promoters methylated cells “the methylome” would greatly advance our understanding regulatory networks tumorigenesis. We previously described new method identifying tumor suppressor based on pharmacologic unmasking the promoter region detection re-expression microarray analysis. In this study, we modified improved selection candidates structure...
NUT midline carcinoma (NMC) is a highly lethal tumor defined by translocations involving the gene on chromosome 15q14. NMC involves structures including sinonasal tract, but its overall incidence at this site and full morphologic profile are largely unknown because tumors not routinely tested for translocation. The recent availability of an immunohistochemical probe protein now permits more complete characterization NMCs. archival files Johns Hopkins Hospital Surgical Pathology were searched...