A5074320825- Hippo pathway signaling and YAP/TAZ
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Wnt/β-catenin signaling in development and cancer
- Mesenchymal stem cell research
- Salivary Gland Disorders and Functions
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Polyomavirus and related diseases
- Kruppel-like factors research
- Aluminum toxicity and tolerance in plants and animals
- Fungal Infections and Studies
- Phagocytosis and Immune Regulation
- Trace Elements in Health
University of Cincinnati Medical Center
2018-2024
The Ohio State University
2018
Copper toxicity and copper limitation can both be effective host defense mechanisms against pathogens. Tolerance of high by fungi makes as a mechanism largely ineffective fungal A forward genetic screen for Histoplasma capsulatum mutant yeasts unable to replicate within macrophages showed the Ctr3 transporter is required intramacrophage proliferation. mediates uptake growth in low copper. Transcription CTR3 gene induced differentiation H. into pathogenic available copper, but not decreased...
Human cytomegalovirus (HCMV) infects up to 80% of the world's population. Here, we show that HCMV infection leads widespread changes in human chromatin accessibility and looping, with hundreds thousands genomic regions affected 48 hours after infection. Integrative analyses reveal HCMV-induced perturbation Hippo signaling through drastic reduction TEAD1 transcription factor activity. We confirm extensive concordant loss binding, active H3K27ac histone marks, looping interactions upon Our...
Abstract Human cytomegalovirus (HCMV) is a β-herpesvirus which ubiquitous in the human population. HCMV has largest genome of all known herpesviruses, and thus encodes large array proteins that affect pathogenesis different cell types. Given ability to replicate range cells, investigators have begun identify viral required for type-specific replication. There are four encoded homologous G protein-coupled receptors (GPCRs); these viral-encoded GPCRs (vGPCRs) UL33, UL78, US27, US28. In current...
Various aspects of human cytomegalovirus (HCMV) pathogenesis, including its ability to replicate in specific cells and tissues the mechanism(s) horizontal transmission, are not well understood, predominantly because strict species specificity exhibited by HCMV. Murine CMV (MCMV), which contains numerous gene segments highly similar those HCMV, has been useful for modeling some pathogenesis; however, it remains essential build relevant cell-based systems investigate how HCMV counterparts...
Human cytomegalovirus (HCMV) tropism for epithelial cells is determined by the pentameric glycoprotein complex found on viral envelope. Laboratory-adapted strains, such as AD169, typically develop loss-of-function mutations pentamer, thus losing ability to efficiently initiate lytic replication in cells. Using our human salivary gland-derived (hSGE) cell model, we observed that 3 chemically distinct histone deacetylase (HDAC) inhibitors can rescue infection hSGE using pentamer-null strains...
The salivary glands are a site of significant interest for researchers interested in multiple aspects human disease. One goal is to restore function damaged by radiation therapies or due pathologies associated with Sjögren's syndrome. A second define the mechanisms which viruses replicate within glandular tissue where they can then gain access fluids important horizontal transmission. These goals highlight need robust and accessible vitro gland model that be utilized above mentioned as well...
Human cytomegalovirus (HCMV) infects up to 80% of the world’s population. Here, we show that HCMV infection leads widespread changes in human chromatin accessibility and looping, with hundreds thousands genomic regions affected 48 hours after infection. Integrative analyses reveal HCMV-induced perturbation Hippo signaling through drastic reduction TEAD1 transcription factor activity. We confirm extensive concordant loss binding, active H3K27ac histone marks, looping interactions upon Our...
Human cytomegalovirus (HCMV) infects up to 80% of the world’s population. Here, we show that HCMV infection leads widespread changes in human chromatin accessibility and looping, with hundreds thousands genomic regions affected 48 hours after infection. Integrative analyses reveal HCMV-induced perturbation Hippo signaling through drastic reduction TEAD1 transcription factor activity. We confirm extensive concordant loss binding, active H3K27ac histone marks, looping interactions upon Our...
The salivary glands are a site of significant interest for researchers interested in multiple aspects human disease. One goal is to restore function damaged by radiation therapies or due pathologies associated with Sjögren's syndrome. A second define the mechanisms which viruses replicate within glandular tissue where they can then gain access fluids important horizontal transmission. These goals highlight need robust and accessible vitro gland model that be utilized above mentioned as well...