A5084211838- Chromium effects and bioremediation
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Hippo pathway signaling and YAP/TAZ
- Eicosanoids and Hypertension Pharmacology
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Immune Response and Inflammation
- Heavy Metal Exposure and Toxicity
- Muscle Physiology and Disorders
- Congenital heart defects research
- Inflammasome and immune disorders
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Neuroscience and Neuropharmacology Research
- Kruppel-like factors research
- Head and Neck Cancer Studies
- Prostate Cancer Treatment and Research
- Bioinformatics and Genomic Networks
- Polyomavirus and related diseases
- Health, Environment, Cognitive Aging
- Viral-associated cancers and disorders
- Lymphoma Diagnosis and Treatment
Cincinnati Children's Hospital Medical Center
2022-2025
University of Cincinnati Medical Center
2015-2022
University of Cincinnati
2016-2017
Persistent infection with HPV causes nearly 5% of all cancers worldwide, including cervical and oropharyngeal cancers. Compared to HPV-negative (HPV−) head neck squamous cell carcinomas (HNSCCs), HPV-positive (HPV+) HNSCCs exhibit a significantly improved treatment response; however, established regimens were largely developed for HPV− disease. Effectively de-escalating therapy optimizing protocols minimize toxicity both HPV+ tumors has been variably successful, in part due the heterogeneity...
Eosinophilic esophagitis (EoE) is a rare atopic disorder associated with esophageal dysfunction, including difficulty swallowing, food impaction, and inflammation, that develops in small subset of people allergies. Genome-wide association studies (GWASs) have identified 9 independent EoE risk loci reaching genome-wide significance (p < 5 × 10
Abstract Background There are two major genetic types of Epstein-Barr Virus (EBV): type 1 (EBV-1) and 2 (EBV-2). EBV functions by manipulating gene expression in host B cells, using virus-encoded regulatory proteins including Nuclear Antigen (EBNA2). While EBNA2 is known to interact with human transcription factors (hTFs) such as RBPJ, EBF1, SPI1 (PU.1), shares only ~ 50% amino acid identity thus may have distinct binding partners, genome locations, functions. Results In this study, we...
Abstract Prostate cancer stem cells (CSC) are implicated in tumor initiation, progression, metastasis, and the development of therapeutic-resistant disease. It is well known that bulk prostate express androgen receptor (AR) androgens required for growth, emergence castration-resistant In contrast, small subpopulation self-renewing CSCs exhibits an AR-negative (AR−) signature. The mechanisms underlying absence AR unknown. Using CSC-like cell models isolated from clinical biopsy tissues, we...
Abstract Persistent HPV16 infection is a major cause of the global cancer burden. The viral life cycle dependent on differentiation program stratified squamous epithelium, but landscape keratinocyte subpopulations which support distinct phases has yet to be elucidated. Here, single cell RNA sequencing infected compared uninfected organoids identifies twelve populations, with subset mapped reconstruct their respective 3D geography in epithelium. Instead conventional terminally differentiated...
Cognate interaction between CD4+ effector memory T (TEM) cells and dendritic (DCs) induces innate inflammatory cytokine production, resulting in detrimental autoimmune pathology storms. While TEM use tumor necrosis factor (TNF) superfamily ligands to activate DCs, whether prompt other DC-intrinsic changes that influence the response has never been investigated. We report surprising discovery trigger double-strand DNA breaks via mitochondrial reactive oxygen species (ROS) production...
Hexavalent chromium compounds are well-established respiratory carcinogens used in industrial processes. While inhalation exposure constitutes an occupational risk affecting mostly workers, environmental from drinking water is a widespread gastrointestinal cancer risk, millions of people throughout the world. Cr(VI) genotoxic, forming protein-Cr-DNA adducts and silencing tumor suppressor genes, but its mechanism action at molecular level poorly understood. Our prior work using FAIRE showed...
Complex mixtures of environmental agents often cause mixture-specific health effects that cannot be accounted for by a single mechanism. To study the biological exposure to mixture chromium-VI and benzo[a]pyrene (B[a]P), found together in environment, we exposed mice 60 days 0, 55, 550, or 5500 ppb Cr(VI) drinking water followed 90 coexposure B[a]P at 1.25, 12.5, 125 mg/kg/day examined liver gastrointestinal (GI) tract effects. In liver, caused more significant histopathology than expected...
Human cytomegalovirus (HCMV) infects up to 80% of the world's population. Here, we show that HCMV infection leads widespread changes in human chromatin accessibility and looping, with hundreds thousands genomic regions affected 48 hours after infection. Integrative analyses reveal HCMV-induced perturbation Hippo signaling through drastic reduction TEAD1 transcription factor activity. We confirm extensive concordant loss binding, active H3K27ac histone marks, looping interactions upon Our...
Multinucleated skeletal muscle cells have an obligatory need to acquire additional nuclei through fusion with activated stem when responding both developmental and adaptive growth stimuli. A fundamental question in biology has been the reason underlying this for new syncytial that already harbor hundreds of nuclei. To begin answer long-standing question, we utilized nuclear RNA-sequencing approaches developed a lineage tracing strategy capable defining transcriptional state recently fused...
Hexavalent chromium compounds are well-established respiratory carcinogens to which humans commonly exposed in industrial and occupational settings. In addition, natural anthropogenic sources of these contribute the exposure global populations through multiple routes, including dermal, ingestion inhalation that elevate risk cancer by largely unresolved mechanisms. Cr(VI) has genotoxic properties include ternary adduct formation with DNA, increases DNA damage, mostly double-strand break...
Human cytomegalovirus (HCMV) infects up to 80% of the world’s population. Here, we show that HCMV infection leads widespread changes in human chromatin accessibility and looping, with hundreds thousands genomic regions affected 48 hours after infection. Integrative analyses reveal HCMV-induced perturbation Hippo signaling through drastic reduction TEAD1 transcription factor activity. We confirm extensive concordant loss binding, active H3K27ac histone marks, looping interactions upon Our...
Human cytomegalovirus (HCMV) infects up to 80% of the world’s population. Here, we show that HCMV infection leads widespread changes in human chromatin accessibility and looping, with hundreds thousands genomic regions affected 48 hours after infection. Integrative analyses reveal HCMV-induced perturbation Hippo signaling through drastic reduction TEAD1 transcription factor activity. We confirm extensive concordant loss binding, active H3K27ac histone marks, looping interactions upon Our...