A5077991708- Microfluidic and Bio-sensing Technologies
- Characterization and Applications of Magnetic Nanoparticles
- Erythrocyte Function and Pathophysiology
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Fluid Dynamics and Mixing
- Magnetic and Electromagnetic Effects
- Electrohydrodynamics and Fluid Dynamics
- Cancer Cells and Metastasis
- Micro and Nano Robotics
- Viral Infectious Diseases and Gene Expression in Insects
- Electrostatics and Colloid Interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Particle Dynamics in Fluid Flows
- Hemoglobinopathies and Related Disorders
- Geomagnetism and Paleomagnetism Studies
- Protein purification and stability
- Blood properties and coagulation
- Iron Metabolism and Disorders
- Field-Flow Fractionation Techniques
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- Fluid Dynamics and Heat Transfer
- Head and Neck Cancer Studies
- Hemoglobin structure and function
The Ohio State University
2016-2025
Zero to Three
2019
Scunthorpe General Hospital
2012
Charles University
2012
Nationwide Children's Hospital
2010
Cleveland Clinic
1998-2009
Bar-Ilan University
2004
University of Toledo
2004
Chalmers University of Technology
2001-2003
Colorado School of Mines
1999
ABSTRACT Extracellular vesicles (EVs) are under evaluation as therapeutics or vehicles for drug delivery. Preclinical studies of EVs often use mice other animal models to assess efficacy and disposition. However, most derived from human cells, they may elicit immune responses which contribute toxicities enhanced EV clearance. Furthermore, different cell sources comprising various cargo differ with respect immunogenicity toxicity. To EV‐induced response toxicity, we dosed C57BL/6...
Magnetic cell separation has become a popular technique to enrich or deplete cells of interest from heterogeneous population. One important aspect magnetic is the degree which binds paramagnetic material. It this material that imparts positive magnetophoretic mobility target cell, thus allowing effective separation. A mathematical relationship been developed correlate labeling an immunomagnetically labeled cell. Four parameters have identified significantly affect cell: antibody binding...
Polylactic acid (PLA) polymer film was degraded in abiotic and biotic environments to understand the role of microbes degradation process lactic based polymers. The studies were conducted a well-characterized system, an sterile aqueous desiccated environment maintained at 40, 50, 60 °C. combination experiments different isolated distinct effect microbes, water, temperature on morphological changes during degradation. Due lack availability radiolabeled PLA, various analytical techniques...
The optimization of a purely negative depletion, enrichment process for circulating tumor cells (CTCs) in the peripheral blood head and neck cancer patients is presented. uses red cell lysis step followed by immunomagnetic labeling, subsequent CD45 positive cells. A number relevant variables are quantified, or attempted to be which control performance process. Six different labeling combinations were evaluated as well significant difference with respect source: buffy coats purchased from Red...
The separation and or isolation of rare cells using magnetic forces are commonly used growing in use ranging from simple sample prep for further studies to a FDA approved, clinical diagnostic test. This growth is the result both demand obtain homogeneous molecular analysis dramatic increases power permanent magnets that even allow some unlabeled based on intrinsic moments, such as malaria parasite-infected red blood cells.
// Joao Paulo Oliveira-Costa 1 , Alex Fiorini de Carvalho 2 Giorgia Gobbi da Silveira Peter Amaya 3 Yongqi Wu Kyoung-Joo Jenny Park Mabel Pinilla Gigliola Maryam Lustberg 4 Marcilei Eliza Cavicchioli Buim 5 Elisa Napolitano Ferreira Luiz Kowalski 6 Jeffrey J. Chalmers Fernando Augusto Soares Dirce Maria Carraro Alfredo Ribeiro-Silva Department of Pathology, Ribeirao Preto School Medicine, University Sao Paulo, Preto, Brazil Laboratory Genomics and Molecular Biology, CIPE, A.C. Camargo Cancer...
Circulating tumor cells (CTCs) are commonly isolated from the blood by targeting epithelial cell adhesion molecule (EpCAM) through positive selection. However, EpCAM can be downregulated during metastatic progression, or it initially not present. We designed present prospective trial to characterize CTCs as well other circulating populations in samples women with breast cancer without EpCAM-dependent enrichment and/or isolation technology.A total of 32 patients were enrolled, and processed...
Abstract A microfluidic device was fabricated via photolithographic techniques which can create transient elongational and shear forces ranging over three orders of magnitude while still maintaining laminar flow conditions. The contractional fluid inside the simulated with FLUENT (a computational dynamics computer program) local deformation were characterized scalar quantity, energy dissipation rate. sensitivities four cell lines (CHO, HB‐24, Sf‐9, MCF7) tested in device. results indicate...
A brief, process-oriented overview of immunologically based cell separation technology is presented. In addition, the design and preliminary experimental data two unique flow-through immunomagnetic devices are The first on a dipole magnetic field, while second basis quadrupole field. can "fractionate" an inlet, magnetically labeled, stream into different outlet streams degree to which immunomagnetically labeled. separator splits in purity, recovery, potentially cells labeled controlled by...
Abstract In this paper, the second in series, use of a microscopic, high‐speed video system to study interactions two suspended insect cells strains, Trichoplusia ni (TN‐368) and Spodoptera frugiperda (SF‐9), with rupturing bubbles is reported. Events such as adsorption onto bubble film mechanism rupture were observed. On basis these observations experimental theoretical work other researchers on cell death result sparging, it proposed that are killed by rapid acceleration after high levels...
Objectives: To present and discuss a high-performance negative depletion method for the isolation of circulating tumor cells (CTCs) in blood patients with head neck cancer to determine correlation between presence CTCs early clinical outcome these patients.Design: Prospective follow-up study squamous cell carcinoma (SCCHN) undergoing surgical intervention, who had peripheral examined CTCs. Patients:The population comprised 48 diagnosed as having SCCHN intervention.Intervention: A process...
Epithelial to mesenchymal transition (EMT) has been hypothesized as a mechanism by which cells change phenotype during carcinogenesis, well tumor metastasis. Whether EMT is involved in cancer metastasis specific, practical impact on the field of circulating (CTCs). Since generally accepted definition CTC includes expression epithelial surface markers, such EpCAM, if cell loses its markers (which suggested EMT), it will not be separated and/or identified CTC. We have developed, and previously...
Abstract A majority of the previous investigations on hydrodynamic sensitivity mammalian cells have focused lethal effects as determined by cell death or lysis. In this study, we investigated effect stress CHO in a fed‐batch process using previously reported system which subjects to repetitive, high levels stress, quantified energy dissipation rate (EDR). The results indicated that growth and monoclonal antibody production test were very resistant stress. Compared control, no significant...
ABSTRACT Extracellular vesicles (EVs) hold great potential as novel systems for nucleic acid delivery due to their natural composition. Our goal was load EVs with microRNA that are synthesized by the cells produce EVs. HEK293T were engineered expressing a lysosomal associated membrane, Lamp2a fusion protein. The gene encoding pre‐miR‐199a inserted into an artificial intron of TAT peptide/HIV‐1 transactivation response (TAR) RNA interacting peptide exploited enhance EV loading containing...
Single cell study is gaining importance because of the cell-to-cell variation that exists within population, even after significant initial sorting. Analysis such at gene expression level could impact single functional genomics, cancer, stem-cell research, and drug screening. The on-chip monitoring individual cells in an isolated environment would prevent cross-contamination, provide high recovery yield, enable biological traits a level. These advantages experiments improvement for myriad...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalytical Magnetapheresis of Ferritin-Labeled LymphocytesMaciej. Zborowski, Chwan Bor. Fuh, Ralph. Green, Liping. Sun, and Jeffrey J. ChalmersCite this: Anal. Chem. 1995, 67, 20, 3702–3712Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/ac00116a014https://doi.org/10.1021/ac00116a014research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract Positive selection of CD34+ blood progenitor cells from circulation has been reported to improve patient recovery in applications autologous transplantation. Current magnetic separation methods rely on cell capture and release solid supports rather than sorting flowing suspensions, which limits the range therapeutic process scale up. We tested immunomagnetic labeling isolation fresh leukocyte fraction peripheral (leukapheresis) using continuous quadrupole flow sorter (QMS),...
During intra-erythrocytic development, malaria trophozoites digest hemoglobin, which leads to parasite growth and asexual replication while accumulating toxic heme. To avoid death, the synthesizes insoluble hemozoin crystals in digestive vacuole through polymerization of beta-hematin dimers. In process, heme is converted a high-spin ferriheme whose magnetic properties were studied as early 1936 by Pauling et al. Here, magnetophoretic cell motion analysis, we provide evidence for graduated...