A5036419714- Child Nutrition and Water Access
- Escherichia coli research studies
- Digestive system and related health
- Viral gastroenteritis research and epidemiology
- Child Nutrition and Feeding Issues
- Clinical Nutrition and Gastroenterology
- Chemotherapy-induced organ toxicity mitigation
- Food Security and Health in Diverse Populations
- Trace Elements in Health
- Nutrition and Health in Aging
- Tuberous Sclerosis Complex Research
- Iron Metabolism and Disorders
- Polyomavirus and related diseases
- Barrier Structure and Function Studies
- Gut microbiota and health
- Nutritional Studies and Diet
- Biochemical Analysis and Sensing Techniques
- Vagus Nerve Stimulation Research
- Infant Nutrition and Health
- Advanced Glycation End Products research
- Cardiac Ischemia and Reperfusion
- Salmonella and Campylobacter epidemiology
- Acute Kidney Injury Research
- Clostridium difficile and Clostridium perfringens research
- Neurological Disorders and Treatments
Universidade Federal do Ceará
2016-2025
Dysfunction of the intestinal epithelium barrier (DIEB) is frequent and can lead to serious complications in early childhood when diagnosis clinical intervention are limited, especially children with environmental enteric disease malnutrition. The use refined analytical techniques increasingly necessary this context. This study aimed validate high-performance liquid chromatography method coupled tandem mass spectrometry (LC-MS/MS) measure DIEB by lactulose:mannitol ratio detection (LM test)...
Diabetic-metabolic syndrome (MetS-D) has a high prevalence worldwide, in which an association with the rupture of intestinal epithelium barrier function (IEBF) been pointed out, but functional and morphological properties are still not well understood. This study aimed to evaluate impact acute hyperglycemia diabetes on tight junction proteins, metabolic failure, ion water transports, IEBF parameters. Diabetes was induced male Rattus norvegicus (200-310 g) 0.5 mL streptozotocin (70 mg/kg)....
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control fed standard II) undernourished RBD. After 7 days, killed collected evaluation electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) protein expression...
Few studies have focused on quantitatively analyzing nutrients from infant diets, compromising complementary feeding evaluation and health promotion worldwide. This study aimed to describe dietary intake in infants 9 24 mo of age, determining nutrient intakes associated with the risk underweight, wasting, stunting. Usual were determined by 24-h recalls collected when 9–24 age communities 7 low- middle-income countries: Brazil (n = 169), Peru 199), South Africa 221), Tanzania 210), Bangladesh...
Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children from developing countries and presents high genetic variability. We aimed to characterize the EPEC virulence-related gene (VRG) distribution copathogens associated with nutrition-related outcomes low-income Brazilian semiarid region. A cross-sectional case-control study was conducted 1,191 aged 2 36 months northeast region Brazil. Stool samples were collected clinical, epidemiological, anthropometric data...
Abstract Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition intestinal barrier function. This study evaluated the impact a diet physical development morphofunctional in mice. C57BL/6 (21 days age) mice were fed Northeastern Brazil regional basic (RBD) or control for 21 d. The animals subjected to bioimpedance analysis, lactulose test, morphometric analysis quantitative reverse transcription-PCR evaluate tight junctions...
Abstract Acrolein is the main toxic metabolite of Ifosfamide (IFO) that causes urothelial damage by oxidative stress and inflammation. Here we investigate molecular mechanism action gingerols, Zingiber officinale bioactive molecules, as an alternative treatment for ifosfamide-induced hemorrhagic cystitis. Female Swiss mice were randomly divided into 5 groups: control; IFO; IFO + Mesna; [8]- or [10]-gingerol. Mesna (80 mg/kg, i.p.) was given minutes before, 4 8 hours after (400mg/kg, i.p.)....
Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed investigate the effects of EPEC infection on intestinal epithelial in vitro, well involvement type III secretion system (T3SS) and Rho GTPases. Crypt cells (IEC-6) were infected with strains (E2348/69, ΔescF, LDI001 strain isolated from a malnourished Brazilian child) commensal E. HS. Wound death assays performed at different time-points. Transcription...
ABSTRACT Background: Enteroaggregative Escherichia coli (EAEC) is an important pathogen causing enteric infections worldwide. This pathotype linked to malnutrition in children from developing countries. Alanyl‐glutamine (Ala‐Gln) immune modulator nutrient that acts during intestinal damage and/or inflammation. study investigated the effect of EAEC infection and Ala‐Gln on cell viability, death, inflammation epithelium cells (IEC‐6). Methods: Cells were infected with prototype 042 strain,...
Ischemia/reperfusion injury (I/R) is commonly related to acute kidney (AKI) and oxidative stress. Antioxidant agents are used treat this condition. Lippia sidoides a brazillian shrub with anti-inflammatory anti-oxidative properties. Thus, the aim of study evaluate effect ethanolic extract (LSEE) on in vivo vitro models AKI induced by I/R. Male Wistar rats were submitted unilateral nephrectomy ischemia contralateral for 60 min via clamping followed reperfusion 48 h. They divided into four...
ABSTRACT Lactulose and mannitol have been used to assess intestinal permeability several methodologies used. Objectives This study aimed validate the high-performance liquid chromatography method coupled with tandem mass spectrometry measure lactulose sugars. Material Methods We a (HPLC) system an ABsciex Q-TRAP 5500 triple quadrupole spectrometer (MS/MS) ABSciex Electro Nebulization Interface (ESI) (Framingham, MA, USA). For separation of compounds in HLPC, analytical column HILIC-ZIC® from...