A5064973744- Viral gastroenteritis research and epidemiology
- Child Nutrition and Water Access
- Escherichia coli research studies
- Clinical Nutrition and Gastroenterology
- Infant Nutrition and Health
- Digestive system and related health
- Viral Infections and Immunology Research
- Pregnancy and Medication Impact
- Public Health in Brazil
- Iron Metabolism and Disorders
- Diet and metabolism studies
- Ginger and Zingiberaceae research
- Child Nutrition and Feeding Issues
- Drug Transport and Resistance Mechanisms
- Animal Virus Infections Studies
- Clostridium difficile and Clostridium perfringens research
- Pharmacological Effects and Toxicity Studies
- Gastrointestinal motility and disorders
- Birth, Development, and Health
- Salmonella and Campylobacter epidemiology
- Vitamin C and Antioxidants Research
- Enterobacteriaceae and Cronobacter Research
- Muscle metabolism and nutrition
- Listeria monocytogenes in Food Safety
- Congenital Anomalies and Fetal Surgery
Universidade Federal do Ceará
2011-2020
University of Virginia
2016
Center for Global Health
2016
Critical to the design and assessment of interventions for enteropathy its developmental consequences in children living impoverished conditions are non-invasive biomarkers that can detect intestinal damage predict effects on growth development. We therefore assessed fecal, urinary systemic predictors 375 6-26 month-old with varying degrees malnutrition (stunting or wasting) Northeast Brazil. 301 these returned followup anthropometry after 2-6m. Biomarkers correlated stunting included plasma...
ABSTRACT Nephrotoxicity is the main complication of gentamicin (GM) treatment. GM induces renal damage by overproduction reactive oxygen species and inflammation in proximal tubular cells. Phenolic compounds from ginger, called gingerols, have been demonstrated to antioxidant anti-inflammatory effects. We investigated if oral treatment with an enriched solution gingerols (GF) would promote a nephroprotective effect animal nephropathy model. The following six groups male Wistar rats were...
Fecal biomarkers have emerged as important tools to assess intestinal inflammation and enteropathy. The aim of this study was investigate the correlations between fecal markers, myeloperoxidase (MPO), lactoferrin (FL), calprotectin (FC) lipocalin-2 (Lcn-2), compare differences by breastfeeding status well normalization protein or weight. Simultaneous, quantitative MPO, FL, FC Lcn-2, levels were determined in frozen specimens collected from 78 children (mean age 15.2 ± 5.3 months) a...
Abstract Acute kidney injury (AKI) and metabolic dysfunction are critical complications in sepsis syndrome; however, their pathophysiological mechanisms remain poorly understood. Therefore, we evaluated whether the pharmacological properties of 6-gingerol (6G) 10-gingerol (10G) could modulate AKI disruption a rat model (faecal peritonitis). Animals from sham groups were intraperitoneally injected with 6G or 10G (25 mg/kg). Septic decreased creatinine clearance renal antioxidant activity, but...
Campylobacter is an important cause of foodborne gastroenteritis. We determined the occurrence jejuni and coli, using culture-based methods PCRs targeting virulence-associated genes (VAGs) among children aged ≤14 years who were treated for diarrhoea at emergency rooms in northeastern Brazil. Genomic DNA was extracted directly from stool samples collected 366 children. A questionnaire also applied to qualify clinical conditions presented by each child time admission. C. coli detected 16.4 %...
Background Diarrheal diseases are an important cause of morbidity and mortality among children in developing countries. We aimed to study the etiology severity diarrhea living low-income semiarid region Brazil. Methodology This is a cross-sectional, age-matched case-control aged 2–36 months from six cities Brazil's region. Clinical, epidemiological, anthropometric data were matched with fecal samples collected for identification enteropathogens. Results enrolled 1,200 children, 596 cases 604...
Malnutrition results in serious consequences for growth and cognitive development children. We studied select child maternal biologic factors, socioeconomic enteric pathogenic burden gut function biomarkers 402 children 6–24 months of age Northeastern Brazil. In this prospective case–control study, not being fed colostrum [odds ratio (OR): 3.29, 95% confidence interval (CI): 1.73–6.26], ≥18 years (OR: 1.88, CI: 1.10–3.22) no electric fan 2.46, 1.22–4.96) or bicycle 1.80, 1.10–2.95) the...
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control fed standard II) undernourished RBD. After 7 days, killed collected evaluation electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) protein expression...
The transport of myo-inositol is the main mechanism for maintenance its high intracellular levels. We aimed to measure mRNA and protein levels cotransporters in sciatic nerve (SN) dorsal root ganglia (DRG) during experimental diabetes. Streptozotocin-induced (STZ; 4, 8, 12 weeks; 65 mg/kg; ip) diabetic rats (DB) age-matched euglycemic (E) were used analysis sodium 1, 2 (SMIT1, SMIT2) or H+/myo-inositol cotransporter (HMIT). There was a significant reduction SMIT1 SN DRG (by 36.9 31.0%)...
Norovirus (NoV) infections are known to have high-morbidity and mortality rates a major health problem globally. The impact of NoV on child development is, however, poorly understood. We evaluated the distribution genotypes in children from low-income Brazilian semiarid region, relation with their clinical symptoms, nutritional status, co-pathogens.The test population included aged 2 36 months 6 cities region. Fecal samples were collected each child, along information regarding...
Scorpion venom causes renal injury and affects vascular ion-channels function. Centruroides margaritatus scorpion is found in Colombia frequently the cause of envenomation accidents; however, its impact has never been investigated.To evaluate effects C. (CmV) on parameters using isolated rat kidney cell culture models.Wistar rats (n = 5, weighing 240-300 g) were first perfused with Krebs-Henseleit solution containing 6 g 100 mL-1 bovine serum albumin. After 30 minutes, kidneys CmV to a final...
Strenuous exercise triggers deleterious effects on the intestinal epithelium, but their mechanisms are still uncertain. Here, we investigated whether a prolonged training and an additional exhaustive protocol alter permeability putative effect of alanyl-glutamine (AG) pretreatment in this condition. Rats were allocated into 5 different groups: 1) sedentary; 2 3) trained (50 min per day, days week for 12 weeks) with or without 6 weeks oral (1.5 g/kg) AG supplementation; 4 5) subjected to test...
Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children from developing countries and presents high genetic variability. We aimed to characterize the EPEC virulence-related gene (VRG) distribution copathogens associated with nutrition-related outcomes low-income Brazilian semiarid region. A cross-sectional case-control study was conducted 1,191 aged 2 36 months northeast region Brazil. Stool samples were collected clinical, epidemiological, anthropometric data...
Epithelial cell migration is an essential response to enteric pathogens such as enteropathogenic Escherichia coli (EPEC). This study aimed investigate the effects of EPEC infection on intestinal epithelial in vitro, well involvement type III secretion system (T3SS) and Rho GTPases. Crypt cells (IEC-6) were infected with strains (E2348/69, ΔescF, LDI001 strain isolated from a malnourished Brazilian child) commensal E. HS. Wound death assays performed at different time-points. Transcription...
ABSTRACT Background: Enteroaggregative Escherichia coli (EAEC) is an important pathogen causing enteric infections worldwide. This pathotype linked to malnutrition in children from developing countries. Alanyl‐glutamine (Ala‐Gln) immune modulator nutrient that acts during intestinal damage and/or inflammation. study investigated the effect of EAEC infection and Ala‐Gln on cell viability, death, inflammation epithelium cells (IEC‐6). Methods: Cells were infected with prototype 042 strain,...
ABSTRACT Background: Rotavirus A (RVA) is one of the leading causes acute gastroenteritis worldwide; however, few studies assessed RVA genetics with community surveillance. Objectives: This study aimed to investigate clinical data, genetic diversity, and coinfection patterns infections in children from 2 36 months old or without childhood diarrhea Brazilian semiarid region during postvaccination era. Methods: We enrolled collected socioeconomic/clinical information using a standardized...