A5062574910- Erythrocyte Function and Pathophysiology
- Blood transfusion and management
- Freezing and Crystallization Processes
- Blood groups and transfusion
- Blood donation and transfusion practices
- Physiological and biochemical adaptations
- Reproductive Biology and Fertility
- Blood properties and coagulation
- nanoparticles nucleation surface interactions
- Digital Imaging for Blood Diseases
- Hemoglobinopathies and Related Disorders
- Icing and De-icing Technologies
- Pluripotent Stem Cells Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Mesenchymal stem cell research
- Hepatitis B Virus Studies
- Point processes and geometric inequalities
- 3D Shape Modeling and Analysis
- Pancreatic function and diabetes
- Hematopoietic Stem Cell Transplantation
- Textile materials and evaluations
- Artificial Intelligence in Healthcare
- Spaceflight effects on biology
- Plant Surface Properties and Treatments
- Reproductive Health and Technologies
Canadian Blood Services
2014-2024
Bristol-Myers Squibb (United States)
2022-2023
University of Alberta
2013-2021
University of Ottawa
2015
Sunnybrook Health Science Centre
2012
Health Sciences Centre
2012
Commonwealth Scientific and Industrial Research Organisation
1987-1993
National Measurement Laboratory
1989
This paper shows how approximate maximum likelihood estimation for fairly general point processes on the line can be performed with GLIM. The approximation is based a weighted sum to an integral in likelihood. Various weighting schemes are briefly examined. methodology illustrated example, and its extension Poisson higher dimensions described.
Abstract In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced rapid freezing rates. After thawing and transfusion, must be removed avoid intravascular hemolysis. This is time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc β-pBrPh-Glc have the...
Canadian Blood Services has been conducting quality monitoring of red blood cell (RBC) components since 2005, a period spanning the implementation semiautomated component production. The aim was to compare RBC produced before and after this production method change.Data from 572 units were analyzed, categorized by method: Method 1, manual methods; 2, buffy coat method; 3, whole filtration method. assessed using an extensive panel in vitro tests, encompassing regulated control criteria such...
Stored red blood cells (RBCs) are needed for life-saving transfusions, but they undergo continuous degradation. RBC storage lesions often assessed by microscopic examination or biochemical and biophysical assays, which complex, time-consuming, destructive to fragile cells. Here we demonstrate the use of label-free imaging flow cytometry deep learning characterize lesions. Using brightfield images, a trained neural network achieved 76.7% agreement with experts in classifying seven clinically...
Abstract During cryopreservation, ice recrystallization is a major cause of cellular damage. Conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol function by number different mechanisms but do not mitigate or control at concentrations utilized in cryopreservation procedures. In North America, human red blood cells (RBCs) utilizes high glycerol. RBC units frozen under these conditions must be subjected to time-consuming deglycerolization process after thawing order...
Background and Objectives While the clinical impact of differences in red blood cell ( RBC ) component processing methods is unknown, there are concerns they may be confounding variables studies such as ongoing ‘age blood’ investigations. Here, we compare vitro characteristics concentrates RCC s) produced by several different methods. Materials Methods Nine were examined: three apheresis (Alyx, MCS + Trima), well leucoreduced whole blood‐derived s buffy coat filtration non‐leucoreduced s....
Abstract Background Characteristics of red blood cells (RBCs) are influenced by donor variability. This study assessed quality and metabolomic variables RBC subpopulations varied biologic age in cell concentrates (RCCs) from male female donors to evaluate their contribution the storage lesion. Study Design Methods Red healthy (n = 6) 4) were Percoll separated into less dense (“young”, Y‐RCCs) (“old”, O‐RCCs) subpopulations, which weekly for 28 days changes hemolysis, mean volume (MCV),...
While irradiation of red cell concentrates (RCC) prevents graft-versus-host disease in susceptible transfusion recipients, it also damages blood cells (RBC). To understand the ability regulations to prevent inferior units, we irradiated 980 RCC saline-adenine-glucose-mannitol (SAGM) using various combinations pre-irradiation age and post-irradiation storage times, measured hemolysis extracellular potassium levels. We observed unacceptably high (>0·8%) some elevated levels all...
BACKGROUND: A 30‐minute rule was established to limit red blood cell (RBC) exposure uncontrolled temperatures during storage and transportation. Also, RBC units issued for transfusion should not remain at room temperature (RT) more than 4 hours (4‐hour rule). This study aimed determining if single or multiple RT exposures affect quality and/or promote bacterial growth. STUDY DESIGN AND METHODS: Growth experiments were performed in RBCs inoculated with Serratia liquefaciens marcescens ....
Low-molecular-weight ice recrystallization inhibitors (IRIs) are ideal cryoprotectants that control the growth of and mitigate cell damage during freezing. Herein, we describe a detailed study correlating inhibition activity cryopreservation ability with structure O-aryl-glycosides. Many effective IRIs efficient cryoadditives for freezing red blood cells (RBCs). One cryoadditive did not inhibit but instead inhibited nucleation, demonstrating significance inhibiting both processes...
Deleterious changes, collectively termed as storage lesions, alter the characteristics of red blood cell (RBC) morphology during in vitro storage. Due to gradual loss cellular membrane, RBCs lose their original biconcave disk shape and begin a process spherical deformation that is characterized by well-defined morphological criteria. At spheroechinocyte stage, structure RBC irreversibly damaged lacks elasticity necessary efficiently deliver oxygen. Quantifying prevalence spheroechinocytes...
On montre que les calculs pour l'estimation des parametres dans le domaine frequences, plusieurs modeles de series temporelles sont essentiellement ceux moindres carres reponderes iterativement et donc peuvent etre realises par la majorite programmes-produits statistiques
Background and Objectives Di‐2‐ethylhexyl phthalate ( DEHP ) is a blood bag plasticizer. It also toxin, raising concerns for vulnerable populations, example, neonates infants. Here, the in vitro quality of red cell concentrates RCC stored paediatric bags formulated with alternative plasticizers to was compared. Materials Methods were pooled split into polyvinylchloride PVC )/ , /1,2‐cyclohexanedicarboxylic acid diisononyl ester DINCH or /butyryl trihexyl citrate BTHC bags. Quality assessed...
Background Nondestructive testing of blood components could permit in‐process quality control and reduce discards. Tubing segments, generated during red cell ( RBC ) component production, were tested to determine their suitability as a sample source for testing. Study Design Methods Leukoreduced produced from whole WB by two different methods: filtration buffy coat BC ). Components corresponding segments on D ays 5 42 hypothermic storage HS spun hematocrit Hct ), hemoglobin Hb content,...
Sterile washing of red blood cells (RBCs) and use an additive solution permits longer postwash storage. The effect irradiation during this extended storage time is unclear.RBCs were washed 14 days after collection using automated cell processor stored in saline-adenine-glucose-mannitol. To determine how long irradiated RBCs could be stored, units 1, 4, 5, 7 vitro quality was assessed. Determined limits for validated. Quality assessment included percent recovery, hemoglobin (Hb), hemolysis,...
We have conducted wearer trials demonstrating that human subjects can perceive the heat of sorption water vapor by wool garments in typical winter conditions. found this causes wearers to rate as warmer under certain conditions than materials with little or no sorption, such acrylic. If are thoroughly dried before use, full benefit heating effect is realized.
BACKGROUND Washed red blood cells (RBCs) are indicated for immunoglobulin (Ig)A‐deficient recipients when RBCs from IgA‐deficient donors not available. Canadian Blood Services recently began using the automated ACP 215 cell processor (Haemonetics Corporation) RBC washing, and its suitability to produce was investigated. STUDY DESIGN AND METHODS produced whole donations by buffy coat (BC) filtration (WBF) methods were washed or COBE 2991 processors IgA total protein levels assessed. A...
Abstract Background Red cell concentrates (RCCs) may be cryopreserved at Canadian Blood Services (CBS) for up to 10 years; however, inadvertent warming of these units over the prescribed storage temperature (≤ −65°C) occur. These discarded from inventory avoid potential adverse transfusion outcomes. This study aimed assess quality RCCs that experienced unintentional transient events (TWEs) related freezer failures. Study Design Thirty with known TWEs were selected this and classified into...
Abstract Background The ACP 215 automated cell processor is used to glycerolize and deglycerolize red concentrates (RCCs). Its primary advantage over the COBE 2991, previously cryopreserve RCCs, that it maintains a closed system enabling extended post‐thaw expiry. However, was observed post‐deglycerolization hematocrits (Hct) of units processed with LN236 kit are markedly lower than those using 2991. Therefore, we intended determine whether modified process smaller volume deglycerolization...
Abstract Background Differences in manufacturing conditions using the Haemonetics ACP 215 cell processor result cryopreserved red concentrates (RCCs) of varying quality. This work studied effect processing method, additive solution, and storage duration on RCC quality to identify an optimal protocol for manufacture RCCs. Materials methods RCCs were pooled‐and‐split stored 7, 14, or 21 days before cryopreservation. Units glycerolized with a single double centrifugation method. After thawing,...
Before transfusion, thawed frozen red cell concentrates (RCCs) must be deglycerolized. In order to ensure that these products meet regulatory standards for hematocrit, an approach manipulate hematocrit post deglycerolization was developed and implemented.Glycerolized RCCs were deglycerolized using the COBE 2991 processor, final product's adjusted by addition of various volumes 0.9% saline / 0.2% dextrose. The in vitro quality (hematocrit, hemolysis, hemoglobin content, volume, recovery, ATP,...
Abstract Units of red blood cell (RBC) concentrates with rare phenotypes are typically not included in method validation studies for cryopreservation processes; rather, they reserved patients needs. Some RBC may demonstrate membrane abnormalities, like acanthocytosis as observed RBCs the McLeod phenotype, and specifically banked these attributes; however, impact that have on post-thaw quality has been well studied. To evaluate how a phenotype is affected by process, 4 units, cryopreserved...