A5072450714- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Hyperthermia Applications
- Flow Measurement and Analysis
- Microfluidic and Bio-sensing Technologies
- Erythrocyte Function and Pathophysiology
- Digital Imaging for Blood Diseases
- Ultrasound Imaging and Elastography
- Blood transfusion and management
- Blood properties and coagulation
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Thermography and Photoacoustic Techniques
- Cancer Cells and Metastasis
- Artificial Intelligence in Healthcare
- Blood groups and transfusion
- Nanoplatforms for cancer theranostics
- Gastric Cancer Management and Outcomes
- ICT Impact and Policies
- ICT in Developing Communities
- Microfluidic and Capillary Electrophoresis Applications
- Thermal Regulation in Medicine
- Electrical and Bioimpedance Tomography
- Nanopore and Nanochannel Transport Studies
- Appendicitis Diagnosis and Management
- Colorectal Cancer Surgical Treatments
Wuhan Engineering Science & Technology Institute
2024
St. Michael's Hospital
2019-2023
Toronto Metropolitan University
2017-2023
University of Toronto
2020-2023
Ted Rogers Centre for Heart Research
2020-2023
Institute for Biomedical Engineering
2022-2023
British Medical Association
1977
Royal College of Psychiatrists
1977
University College London
1977
Stored red blood cells (RBCs) are needed for life-saving transfusions, but they undergo continuous degradation. RBC storage lesions often assessed by microscopic examination or biochemical and biophysical assays, which complex, time-consuming, destructive to fragile cells. Here we demonstrate the use of label-free imaging flow cytometry deep learning characterize lesions. Using brightfield images, a trained neural network achieved 76.7% agreement with experts in classifying seven clinically...
Abstract We describe a new technique that combines ultrasound and microfluidics to rapidly size count cells in high-throughput label-free fashion. Using 3D hydrodynamic flow focusing, are streamed single file through an beam where scattering events from each individual cell acquired. The operates at center frequency of 375 MHz with wavelength 4 μm; when the is similar scatterer, power spectra backscattered waves have distinct features specific frequencies directly related size. Our approach...
While the nucleus-to-cytoplasmic (N:C) ratio has traditionally been used for assessing cell malignancy, most N:C measurement techniques are time-consuming and performed on thin histological sections, which prohibit assessment of three-dimensional structure. A combined ultrahigh frequency ultrasound (US) photoacoustic (PA) technique was to assess size cultured cancer cells in three dimensions (3D). The diameters their stained nuclei were obtained by fitting power spectrum backscattered US...
Deleterious changes, collectively termed as storage lesions, alter the characteristics of red blood cell (RBC) morphology during in vitro storage. Due to gradual loss cellular membrane, RBCs lose their original biconcave disk shape and begin a process spherical deformation that is characterized by well-defined morphological criteria. At spheroechinocyte stage, structure RBC irreversibly damaged lacks elasticity necessary efficiently deliver oxygen. Quantifying prevalence spheroechinocytes...
The development of novel anticancer therapies warrants the parallel biomarkers that can quantify their effectiveness. Photoacoustic imaging has potential to measure changes in tumor vasculature during treatment. Establishing accuracy requires direct comparisons with gold histological standards. In this work, we explore whether a new class submicron, vascular disrupting, ultrasonically stimulated nanobubbles enhance radiation therapy. vivo experiments were conducted on mice bearing prostate...
Abstract Blood transfusion is a life-saving clinical procedure. With millions of units needed globally each year, it growing concern to improve product quality and recipient outcomes. Stored red blood cells (RBCs) undergo continuous degradation, leading structural biochemical changes. To analyze RBC storage lesions, complex biophysical assays are often employed. We demonstrate that label-free imaging flow cytometry deep learning can characterize morphologies during 42-day storage, replacing...
Red blood cells (RBCs) stored in hypothermic environments for the purpose of transfusion have been documented to undergo structural and functional changes over time. One sign so-called RBC storage lesion is irreversible damage cell membrane. Consequently, RBCs a morphological transformation from regular, deformable biconcave discocytes rigid spheroechinocytes. The spherically shaped lack deformability efficiently enter microvasculature, thereby reducing capacity oxygenate tissue. Blood banks...
The nucleus-to-cytoplasm ratio (N:C) can be used as one metric in histology for grading certain types of tumor malignancy. Current N:C assessment techniques are time-consuming and low throughput. Thus, high-throughput clinical contexts, there is a need technique that assess cell malignancy rapidly. In this study, we the four different malignant lines (OCI-AML-5—blood cancer, CAKI-2—kidney HT-29—colon SK-BR-3—breast cancer) non-malignant line (MCF-10A –breast epithelium) using an imaging flow...
A cell’s nucleus-to-cytoplasm ratio (N:C) can be used as a metric in histology for staging malignancy. Current techniques N:C assessment are time-consuming, low-throughput, and lack 3-D structure assessment. In this study, we assess the of MCF-7 cells using an ultra-high frequency acoustic/photoacoustic (PA) microscope compare measurements to imaging flow cytometer (IFC). were stained with DRAQ-5 nuclear dye facilitate production fluorescence PA signals. (PAM) consisting 375 MHz transducer...
During the in vitro storage of red blood cells (RBCs), unfavourable changes (storage lesions) cause a rapid consumption intracellular diphosphoglycerate. The latter deregulates oxygen-haemoglobin binding potential, subsequently increasing oxygen saturation (SO2 ) and membrane degradation, transforming RBCs from biconcave discs to rigid spherical bodies (spheroechinocytes). Current laboratory techniques invasively extract RBC samples assess quality cell concentrate (RCC) units. Optical...
<p><strong>Significance</strong></p> <p>We developed a strategy to avoid human subjectivity by assessing the quality of red blood cells using imaging flow cytometry and deep learning. We successfully automated traditional expert assessment training computer with example images healthy unhealthy morphologies. However, we noticed that experts disagree on ∼18% cells, so instead relying experts’ visual assessment, taught deep-learning network degradation phenotypes...
<p>The development of novel anticancer therapies warrants the parallel biomarkers that can quantify their effectiveness. Photoacoustic imaging has potential to measure changes in tumor vasculature during treatment. Establishing accuracy requires direct comparisons with gold histological standards. In this work, we explore whether a new class submicron, vascular disrupting, ultrasonically stimulated nanobubbles enhance radiation therapy. vivo experiments were conducted on mice bearing...
<p><strong>Significance</strong></p> <p>We developed a strategy to avoid human subjectivity by assessing the quality of red blood cells using imaging flow cytometry and deep learning. We successfully automated traditional expert assessment training computer with example images healthy unhealthy morphologies. However, we noticed that experts disagree on ∼18% cells, so instead relying experts’ visual assessment, taught deep-learning network degradation phenotypes...
<p>Deleterious changes, collectively termed as storage lesions, alter the characteristics of red blood cell (RBC) morphology during in vitro storage. Due to gradual loss cellular membrane, RBCs lose their original biconcave disk shape and begin a process spherical deformation that is characterized by well-defined morphological criteria. At spheroechinocyte stage, structure RBC irreversibly damaged lacks elasticity necessary efficiently deliver oxygen. Quantifying prevalence...
<p>The development of novel anticancer therapies warrants the parallel biomarkers that can quantify their effectiveness. Photoacoustic imaging has potential to measure changes in tumor vasculature during treatment. Establishing accuracy requires direct comparisons with gold histological standards. In this work, we explore whether a new class submicron, vascular disrupting, ultrasonically stimulated nanobubbles enhance radiation therapy. vivo experiments were conducted on mice bearing...
<p>Deleterious changes, collectively termed as storage lesions, alter the characteristics of red blood cell (RBC) morphology during in vitro storage. Due to gradual loss cellular membrane, RBCs lose their original biconcave disk shape and begin a process spherical deformation that is characterized by well-defined morphological criteria. At spheroechinocyte stage, structure RBC irreversibly damaged lacks elasticity necessary efficiently deliver oxygen. Quantifying prevalence...
We have developed an acoustic flow cytometer that combines ultrasound and microfluidics to rapidly size count cells label-free in a high-throughput manner. Cells are hydrodynamically focused single file into narrow stream through beam where is scattered from each individual cell. The operates at center frequency of 375 MHz with wavelength 4 μm; this regime, the power spectra backscattered waves distinct features specific frequencies directly related cell size. Our approach can be used...
Abstract Acoustic properties of biomaterials and engineered tissues reflect their structure cellularity. High-frequency ultrasound (US) can non-invasively characterize monitor these with sub-millimetre resolution. We present an approach to estimate the acoustic cell-laden hydrogels that accounts for frequency-dependent effects attenuation in coupling media, hydrogel thickness, interfacial transmission/reflection coefficients US waves, all which bias estimates. Cell-seeded fibrin disks were...
<p>The nucleus-to-cytoplasm ratio (N:C) can be used as one metric in histology for grading certain types of tumor malignancy. Current N:C assessment techniques are time-consuming and low throughput. Thus, high-throughput clinical contexts, there is a need technique that assess cell malignancy rapidly. In this study, we the four different malignant lines (OCI-AML-5—blood cancer, CAKI-2—kidney HT-29— colon SK-BR-3—breast cancer) non-malignant line (MCF-10A –breast epithelium) using an...
We are reporting two cases with similar appearances on post chemo radiotherapy MRI scan of the mucinous adenocarcinoma. There was high signal T2-weighted sequences mucin pools within and beyond rectal wall. Does this imply that wall tissues become less resistant to cancer intrusion after radiation? intramural pressure have an impact protrusion muscularis propria?
A cell's nucleus-to-cytoplasm (N:C) ratio is a histological metric used to stage malignant disease. Current N:C assessment methods, such as optical microscopy, are time-consuming, subjective, and low-throughput. Here, we compare the ratios of prostate cancer (PC-3) cells measured by novel microfluidic PhotoAcoustic Flow Cytometer (PAFC) those obtained using an Imaging (IFC). PC-3 were stained with DRAQ-5 nuclear dye divided into populations PAFC IFC. The consisted device integrated...