A5022079510- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Endometriosis Research and Treatment
- Nicotinic Acetylcholine Receptors Study
- Osteoarthritis Treatment and Mechanisms
- Ion channel regulation and function
- Bone and Dental Protein Studies
- Cytokine Signaling Pathways and Interactions
- Reproductive Physiology in Livestock
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Hypothalamic control of reproductive hormones
- Immune Response and Inflammation
- Marine Toxins and Detection Methods
- Galectins and Cancer Biology
- RNA Research and Splicing
- Veterinary Equine Medical Research
- Neonatal Respiratory Health Research
- TGF-β signaling in diseases
- Mitochondrial Function and Pathology
- Infant Nutrition and Health
- Pregnancy and preeclampsia studies
- Metastasis and carcinoma case studies
- Myasthenia Gravis and Thymoma
- Epigenetics and DNA Methylation
University of Rijeka
2011-2020
Baylor College of Medicine
1999-2015
The University of Texas MD Anderson Cancer Center
1994
Progesterone regulates reproductive function through two intracellular receptors, progesterone receptor-A (PR-A) and receptor-B (PR-B), that arise from a single gene as transcriptional regulators of progesterone-responsive genes. Although in vitro studies show PR isoforms can display different regulatory activities, their physiological significance is unknown. By selective ablation PR-A mice, we the PR-B isoform modulates subset functions by regulation target Thus, are functionally distinct...
Progesterone (P) regulates female reproduction via two nuclear receptors, PR-A and PR-B. Although both receptors display overlapping distinct transcription regulatory properties, their individual physiological roles are unclear. To address the role of PR-A, we generated a mouse model in which expression PR-B was specifically ablated (PRBKO – / ). We show that selective activation PRBKO mice is sufficient to elicit normal ovarian uterine responses P but results reduced mammary gland...
Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected may fail to implant although many first breach the endometrial epithelium and embed in decidualizing stroma before being rejected via mechanisms are poorly understood. Here we show developmentally impaired human elicit an endoplasmic stress response decidual cells. A was also evident upon vivo exposure of mouse uteri culture medium conditioned by low-quality embryos. By contrast,...
Decidualization renders the endometrium transiently receptive to an implanting blastocyst although underlying mechanisms remain incompletely understood. Here we show that human endometrial stromal cells (HESCs) rapidly release IL-33, a key regulator of innate immune responses, upon decidualization. In parallel, differentiating HESCs upregulate IL-33 transmembrane receptor ST2L and other pro-inflammatory mediators before mounting profound anti-inflammatory response includes downregulation...
Using the recently generated mutant mice strain (PRKO) carrying a null mutation for progesterone receptor (PR) gene by targeting, we examined critical role of PR as coordinator key regulatory events involved in steroid hormone and dopamine-facilitated sexual behavior female mice. In vitro one-point binding analyses estradiol benzoate (EB)-induced cellular PRs immunohistochemistry mediobasal hypothalamus demonstrated reduction homozygous females, equivalent to background levels seen...
Abstract Among the predisposing factors to osteoarthritis (OA), a frequent destructive joint disease, is complex genetic heritage including interleukin‐1 family members like IL1β (IL1B) and IL1 receptor antagonist (IL1RN) genes. The aim of this study was investigate allelic genotypic frequencies IL1B gene single nucleotide polymorphism (SNP) at −511(G>A) variable number tandem repeat (VNTR) in IL1RN Croatian Caucasian population hip OA (HOA) cases healthy controls. A total 259 HOA...
Neurobehavioral effects of progesterone are mediated primarily by its interaction with neural receptors (PRs), expressed as PR-A and PR-B protein isoforms. Whereas the expression two isoforms in tissues is suggestive their selective cellular responses modulation distinct subsets PR-induced target genes, role individual brain behavior unknown. We have previously demonstrated a critical for PRs transcriptional mediators (ligand-dependent), dopamine (ligand-independent)-facilitated female...
The area around Cys‐192 and Cys‐193 is thought to be a functionally important part of the α‐subunit acetylcholine receptor. We have synthesized peptide α 182‐198 α‐chain Torpedo californica receptor investigated binding α‐bungarotoxin, cobratoxin antibodies raised against results showed that synthetic contains second toxin‐binding region also binds considerable fraction anti‐receptor antibodies. report here activity 125–148 human which has been previously localized as in
Decidualizing human endometrial stromal cells (HESCs) profoundly up-regulate 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), the enzyme that converts inert cortisone to active cortisol. We postulated induction of a cortisol gradient upon decidualization periimplantation endometrium may impact on uterine natural killer (uNK) cell population and local expression corticosteroid-dependent target genes.Midluteal biopsies (n = 55) were processed for uNK (CD56) analysis primary HESC cultures....
In rodents, progesterone receptors (PRs) A and B have different often nonoverlapping roles, this study asked whether activities of the PR proteins in mouse are related to differences their expression reproductive tissues. The individual PRA PRB was determined immunohistochemically mammary gland uterus during estrous cycle or response endocrine manipulation. gland, were colocated PR+ epithelial cells, with little change cycle. uterus, not detected luminal epithelium at any stage cycle, cells...
Abstract A series of eighteen consecutive overlapping synthetic peptides, uniform size and overlaps, which encompass the entire extracellular part (residues 1–210) Torpedo californica acetylcholine receptor α chain were examined in vitro for their ability to stimulate lymph node cells from receptor‐primed C57BL/6 (H‐2 b ), C3H/He k SWR q ) SJL s mice. The recognition sites (T sites) by these mouse strains resided within six regions on chain. Three recognized T conincided with antibodies (...
MUC1 expression responds differently to changes in progesterone (P) levels mouse vs. human uterine epithelium. Two isoforms of receptor, PRA and PRB, mediate the physiological effects P. Using transient transfection a epithelial cell line, HEC-1A, we showed that liganded PRB stimulated gene activity. alone had little effect on promoter activity, but antagonized PRB-mediated stimulation. The region from 523 570 bp upstream transcriptional start site was shown be required for P response....
Lactoferrin is a member of the transferrin family iron-binding glycoproteins. induced by estrogen in mouse uterus during early pregnancy. However, expression and function, if any, lactoferrin preimplantation embryo this developmental period has not been investigated. In current study, spatiotemporal murine embryogenesis was examined using situ hybridization immunohistochemical analyses. first detected 2-4 cell fertilized continued until blastocyst stage development. Interestingly, at 16-cell...
Reaction of overlapping synthetic peptides spanning the extracellular part (residues 1-210) alpha chain Torpedo californica acetylcholine receptor (an 2 beta gamma delta pentamer) with anti-receptor antibodies produced profiles continuous antigenic regions correlate segment. Essentially similar were recognized by rabbit and outbred mouse against isolated or membrane-bound receptor. The sites reside within eight regions: residues 1-14, 25-36, 41-53, 63-75, 102-114, 128-138, 172-182, 188-198....
The continuous alpha-neurotoxin-binding regions on the extracellular part (residues 1-210) of alpha-chain Torpedo californica acetylcholine receptor were localized by reaction 125I-labelled alpha-bungarotoxin with synthetic overlapping peptides spanning this entire chain. specificity binding was confirmed inhibition unlabelled toxin and, for appropriate peptides, anti-(acetylcholine receptor) antibodies. Five toxin-binding within residues 1-10, 32-41, 100-115, 122-150 and 182-198. third,...
Genetic predisposition to the complex hereditary disease like osteoarthritis (OA) of large joints (hip and knee) includes interleukin-1 gene (IL-1) cluster on chromosome 2. Using a case-control study with 500 OA patients (240 knee 260 hip patients, all joint replacement), we analysed frequencies IL-1 polymorphisms in Croatian Caucasian population. The control samples came from 531 healthy individuals including blood donors. We genotyped two single nucleotide locus at IL-1A (-889, C>T,...