A5077161899- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Advanced biosensing and bioanalysis techniques
- Genetics, Bioinformatics, and Biomedical Research
- Phytase and its Applications
- S100 Proteins and Annexins
- Origins and Evolution of Life
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- Cell Adhesion Molecules Research
- Electrochemical Analysis and Applications
- Spectroscopy and Quantum Chemical Studies
- Genomics and Phylogenetic Studies
- Protein Tyrosine Phosphatases
- Endoplasmic Reticulum Stress and Disease
- Signaling Pathways in Disease
- Various Chemistry Research Topics
- Redox biology and oxidative stress
- Polysaccharides and Plant Cell Walls
- Advanced Physical and Chemical Molecular Interactions
- Biosensors and Analytical Detection
- Legume Nitrogen Fixing Symbiosis
- Atomic and Molecular Physics
University of Calgary
2007-2019
Starch is the major carbohydrate reserve in plants, and degraded for growth at night. breakdown requires reversible glucan phosphorylation granule surface by novel dikinases phosphatases. The dual-specificity phosphatase starch excess 4 (SEX4) required desphosphorylation; however, regulation of enzymatic activity SEX4 not well understood. We show that switches between reduced (active) oxidized (inactive) states, suggesting redox-regulated. Although only partial reactivation was achieved...
In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded at night to fuel growth metabolism. Starch primarily by β-amylases, liberating maltose, but this activity preceded glucan phosphorylation accompanied dephosphorylation. A phosphatase family member, LIKE SEX4 1 (LSF1), binds required for normal degradation, its exact role unclear. Here, we show that LSF1 does not dephosphorylate glucans. The recombinant dual specificity (DSP) domain of had no...
It is widely accepted that a pair of EF-hands the functional unit typical four EF-hand proteins such as calmodulin or troponin C. In this work we investigate structure and stability domains in related protein calcium- integrin-binding 1 (CIB1) presence absence Mg2+ Ca2+, to determine if similar interactions occur. The backbone flexibility CIB1 were first studied by NMR spectroscopy, these studies complimented with steady-state fluorescence spectroscopy chemical denaturation experiments using...
Mg+2/Mn+2-dependent type 2C protein phosphatases (PP2Cs) are ubiquitous in eukaryotes, mediating diverse cellular signaling processes through metal ion catalyzed dephosphorylation of target proteins. We have identified a distinct PP2C sequence class ("PP2C7s") which is nearly universally distributed Eukaryotes, and therefore apparently ancient. PP2C7s by far most prominent plants green algae. Combining phylogenetic analysis, subcellular localization predictions, distillation publically...
Rapid on-site measurements of arsenic (As) are essential for the timely remediation As-contaminated groundwater both municipal and emergency response applications. Current field tests suffer from either complicated end-user instructions or a lack accuracy specificity. The system presented here combines whole-cell bacterial biosensor with an electrochemical measurement that provides enhanced signal intensity compared to traditional bacterial-detection approaches. When integrated within...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOMLeT—An Alternative Approach to Learning Metabolism: Glycolysis and the TCA Cycle as an ExampleMichael G. Surette , Charles M. Stevens Dylan Silver Brad Behm Raymond J. Turner View Author Information Department of Medical Microbiology Infectious Diseases, University Calgary, Alberta T2N 1N4, Canada Biological Sciences, CanadaCite this: Chem. Educ. 2007, 84, 12, 2024Publication Date (Web):December 1, 2007Publication History Received3 August...