A5044265878- Nuclear Physics and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Radiation Detection and Scintillator Technologies
- Spectroscopy and Chemometric Analyses
- Listeria monocytogenes in Food Safety
- Bacterial Identification and Susceptibility Testing
- Nuclear reactor physics and engineering
- Particle Detector Development and Performance
- Radioactive contamination and transfer
- Advanced Semiconductor Detectors and Materials
- Nuclear Materials and Properties
- Radioactivity and Radon Measurements
- Bee Products Chemical Analysis
- Biosensors and Analytical Detection
- Advanced X-ray and CT Imaging
- Machine Learning in Materials Science
- Medical Imaging Techniques and Applications
- Digital and Cyber Forensics
- Explainable Artificial Intelligence (XAI)
- Non-Destructive Testing Techniques
- Computational Geometry and Mesh Generation
- Microfluidic and Bio-sensing Technologies
- Data Management and Algorithms
- Digital Holography and Microscopy
- Image Processing and 3D Reconstruction
CEA LIST
2009-2024
CEA Cadarache
2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2006-2022
Laboratoire d’Analyse et de Mathématiques Appliquées
2022
Université Paris-Saclay
2021-2022
CEA Paris-Saclay
2002-2009
Organisation de Coopération et de Développement Economiques
2005
CEA Paris-Saclay - Etablissement de Saclay
2002
Raman spectrometry appears to be an opportunity perform rapid tests in microbiological diagnostics as it provides phenotype-related information from single bacterial cells thus holding the promise of direct analysis clinical specimens without any time-consuming growth phase. Here, we demonstrate feasibility a antibiotic-susceptibility determination based on use spectra acquired cells. After two-hour preculture step, one susceptible and two resistant E. coli strains were incubated, for only...
We report on rapid identification of single bacteria using a low-cost, compact, Raman spectroscope.We demonstrate that 60-s procedure is sufficient to acquire comprehensive spectrum in the range 600 3300 cm -1 .This time includes localization small aggregates, alignment individual, and spontaneous scattering signal collection.Fast typically composed less than dozen individuals, achieved by lensfree imaging over large field view 24 mm 2 .The image also allows precise with probing beam without...
Decreasing turnaround time is a paramount objective in clinical diagnosis. We evaluated the discrimination power of Raman spectroscopy when analyzing colonies from 80 strains belonging to nine bacterial and one yeast species directly on solid culture medium after 24-h (macrocolonies) 6-h (microcolonies) incubation. This approach, that minimizes sample preparation time, would allow resuming identification perform downstream antibiotic susceptibility testing. Correct rates measured for...
In this paper we present a longitudinal study of bacteria metabolism performed with novel Raman spectrometer system. Longitudinal is possible our setup since the overall procedure to localize single bacterium and collect spectrum lasts only 1 minute. Localization detection are by means lensfree imaging, whereas signal (from 600 3200 cm<sup>-1</sup>) collected into prototype that allows high light throughput (HTVS technology, Tornado Spectral System). Accomplishing time-lapse spectrometry...
The IGA code (a French acronym standing for actinides gamma isotopy) is a tool developed by the CEA LIST to determine isotopic composition of plutonium and uranium, based on automatic analysis γ/X spectrum emitted nuclear sample. Its main feature its generic approach problem. As consequence, very flexible tool, because no particular experimental setup imposed user acquisitions, in terms energy range, gain, channel number or detector resolution. However, these conditions can have an impact...
The micropattern gaseous detector Micromegas has been developed for several years in Saclay and presents good performance neutron detection. A prototype imaging designed new results obtained thermal beams are presented. Based on previous demonstrating a 1D spatial resolution, tomographic image of multiwire cable performed using prototype. number pillars supporting the micromesh is too large leads to local losses efficiency that distort reconstruction. Nevertheless, this first achieved with...
Timely microbiological results are essential to allow clinicians optimize the prescribed treatment, ideally at initial stage of therapeutic process. Several approaches have been proposed solve this issue and provide result in a few hours directly from sample such as molecular biology. However fast sensitive those methods not based on single phenotypic information which presents several drawbacks limitations. Optical advantage single-cell sensitivity probe phenotype measured cells. Here we...
<title>Abstract</title> Here we present an innovative approach for detecting threat materials within a sealed container by integrating tagged fast neutron activation analysis with Explainable Artificial Intelligence (XAI). Two AI models, Feed-Forward Neural Network (FFNN) and Convolutional (CNN), were developed to analyze the emitted gamma rays identify like explosives drugs based on depth profiles of carbon, nitrogen, oxygen concentrations. XAI was applied make models' decision-making...
In this paper we present results on single bacteria rapid identification obtained with a low-cost and compact Raman spectrometer. At present, demonstrate that 1 minute procedure, including the localization of bacterium, is sufficient to acquire comprehensive spectrum in range 600 3300 cm<sup>-1</sup>. Localization detection performed by means lensfree imaging over large field view 24 mm<sup>2</sup>. An excitation source 532 nm 30 mW illuminates collect signal into Tornado Spectral Systems...
The sIGAle code is developed by CEA LIST at the request of DEN to analyse spectra measured with CdZnTe detectors. a specific automatic analysis software which allows complete spectrum, calibration, identification radionuclides present in determination fluence rates for each significant peak and, if transfer function known, calculation activity radionuclide. takes into account asymmetric peaks tails detector and provides reproducible evaluation areas, was major requirement DEN. has been...
We report on our recent results robust identification of single bacterial cells embedded in various environments using Spontaneous Raman Scattering. Five species bacteria were considered, two which (B. Subtilis and E. Coli) grown under conditions, or real-world matrices. recorded the spectra with a confocal instrument developed lab, performed at level. Our system integrates Lensfree imaging module that allows fast detection over large Field-Of-View. Identification rates comparable to those...
The topic of this study is to build a 2D or 3D thermal neutron tomography system with good spatial resolution (better than σ = 100 μm) for imaging objects some centimeters diameter. Two ways detection have been investigated: Image Plate technology and Micromegas, detector developed particle physics, 50 μm thick foil Lithium as converter. With the classical technology, around has obtained but it would be very difficult develop an industrial based on kind detector. In case Micromegas detector,...
The IGA code (a French acronym standing for actinides gamma isotopy) is a tool developed by the CEA LIST to determine isotopic composition of plutonium and uranium, based on automatic analysis γ/X spectrum emitted nuclear sample. Its main feature its generic approach problem. As consequence, very flexible tool, because no particular experimental setup imposed user acquisitions, in terms energy range, gain, channel number or detector resolution. However, these conditions can have an impact...
Spontaneous Raman scattering is a reliable technique for fast identification of single bacterial cells, when spectra are acquired in laboratory conditions where bacteria growth and state controlled.We have developped multi-modal system combining spectroscopy darkfield imaging, aiming at analysing environmental samples, typically the field context biological pathogens detection.Such samples heterogeneous, both terms phenotype content matrix, even after preliminary purification step.In this...
Due to its performances for neutron detection, the micropattern gaseous detector Micromegas is designed imaging. In this paper will be presented new results obtained with thermal neutrons near Orphee reactor at CEA Saclay. The goal of studies a bi-dimensional was tune different parameters in order achieve best spatial resolution which around 160 mum (defined as standard deviation) double amplification mode and 180 classical mode. Several 2D imaging tests have been carried out an example,...
The sIGAle code is developed by CEA LIST at the request of DEN to analyse spectra measured with CdZnTe detectors. a specific automatic analysis software which allows complete spectrum, calibration, identification radionuclides present in determination fluence rates for each significant peak and, if transfer function known, calculation activity radionuclide. takes into account asymmetric peaks tails detector and provides reproducible evaluation areas, was major requirement DEN. has been...
Nuclear waste management is a major challenge for the nuclear industry. Radiological characterization of packages an important step this industrial process as inaccurate analysis could lead to over classification package and, consequently, higher cost storage. In context, INNOWTECH and CEA List develop low-cost automated measurement method based on gamma spectrometry carried out with CdZnTe detectors in view reducing uncertainties radionuclide activities. previous study, we showed that MCNP6...