A5004546666- Bacterial Identification and Susceptibility Testing
- Cellular transport and secretion
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Advanced Chemical Sensor Technologies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Toxin Mechanisms and Immunotoxins
- Image Processing Techniques and Applications
- Lipid Membrane Structure and Behavior
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Gas Sensing Nanomaterials and Sensors
- Microbial Inactivation Methods
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Advanced X-ray Imaging Techniques
- stochastic dynamics and bifurcation
- Microbial Community Ecology and Physiology
- Electrostatics and Colloid Interactions
bioMérieux (France)
2010-2023
bioMérieux (Brazil)
2014
Systems Technology (United States)
2014
Laboratoire des systèmes et applications des technologies de l'information et de l'énergie
2012
Institut Mérieux (France)
2012
CEA Grenoble
2004-2010
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2004-2010
CEA LETI
2007
Institut Curie
1998-2003
Centre National de la Recherche Scientifique
1998-2003
The molecular mechanisms underlying early/recycling endosomes-to-TGN transport are still not understood. We identified interactions between the TGN-localized putative t-SNAREs syntaxin 6, 16, and Vti1a, two endosomal v-SNAREs, VAMP3/cellubrevin, VAMP4. Using a novel permeabilized cell system, these proteins were functionally implicated in post-Golgi retrograde step. function of Rab6a' was also required, whereas its closely related isoform, Rab6a, has previously been Golgi-to-endoplasmic...
Shiga toxin and other toxins of this family can escape the endocytic pathway reach Golgi apparatus. To synchronize endosome to transport, B-fragment was internalized into HeLa cells at low temperatures. Under these conditions, protein partitioned away from markers destined for late colocalized extensively with cointernalized transferrin. Upon subsequent incubation 37°C, ultrastructural studies on cryosections failed detect B-fragment–specific label in multivesicular or multilamellar...
We visualized a fluorescent-protein (FP) fusion to Rab6, Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi cell periphery. TCs specifically accumulated retrograde cargo, wild-type Shiga toxin B-fragment (STB), during STB endoplasmic reticulum (ER). associated intimately ER, and entered ER via specialized peripheral regions that FP-Rab6. Microinjection of antibodies block...
In HeLa cells, Shiga toxin B-subunit is transported from the plasma membrane to endoplasmic reticulum, via early endosomes and Golgi apparatus, circumventing late endocytic pathway. We describe here that in cells derived human monocytes, i.e., macrophages dendritic was internalized a receptor-dependent manner, but retrograde transport biosynthetic/secretory pathway did not occur part of protein degraded lysosomes. These differences correlated with observation associated Triton...
Raman spectrometry appears to be an opportunity perform rapid tests in microbiological diagnostics as it provides phenotype-related information from single bacterial cells thus holding the promise of direct analysis clinical specimens without any time-consuming growth phase. Here, we demonstrate feasibility a antibiotic-susceptibility determination based on use spectra acquired cells. After two-hour preculture step, one susceptible and two resistant E. coli strains were incubated, for only...
Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This application illustrated by analysis human pathogen Staphylococcus aureus, demonstrating its promise rapid bacteria from positive blood cultures...
Decreasing turnaround time is a paramount objective in clinical diagnosis. We evaluated the discrimination power of Raman spectroscopy when analyzing colonies from 80 strains belonging to nine bacterial and one yeast species directly on solid culture medium after 24-h (macrocolonies) 6-h (microcolonies) incubation. This approach, that minimizes sample preparation time, would allow resuming identification perform downstream antibiotic susceptibility testing. Correct rates measured for...
Despite recent advances in molecular diagnostics, ultrafast determination of the antibiotic susceptibility phenotype pathogenic microorganisms is still a major challenge vitro diagnostics (IVD) infectious diseases. Raman microspectroscopy has been proposed as means to achieve this goal. Previous studies have shown that phenotyping could be done through analysis microbial cells, either large clusters or down single-cell level case Gram-negative rods. Gram-positive cocci such Staphylococcus...
On the road towards efficient diagnostics of infectious diseases, sample preparation is considered as key step and remains a real technical challenge. Finding new methods for extraction micro-organisms from complex biological major challenge prior to pathogen detection analysis. This paper reports technique capturing isolating sample. To achieve segregation pathogens blood cells, dielectrophoretic forces applied bioparticles previously subjected an osmotic shock are successfully implemented...
HIV‐1 Nef protein down‐regulates several important immunoreceptors through interactions with components of the intracellular sorting machinery. expression is also known to induce modifications endocytic pathway. Here, we analyzed effects on retrograde transport, from plasma membrane endoplasmic reticulum using Shiga toxin B‐subunit (STxB). inhibited access STxB reticulum, but did not modify surface level receptor, Gb 3 , nor its internalization rate as measured a newly developed assay....
An innovative and low-cost method is proposed for the detection discrimination of indole-positive pathogen bacteria. The allows non-invasive gaseous indole, released by bacteria, with nanoporous colorimetric sensors. innovation comes from use matrices doped 4-(dimethylamino)-cinnamaldehyde, which act as sponges to trap concentrate targeted analyte turn transparent dark green, long before colonies get visible naked eyes. With such sensors, it was possible discriminate E. coli H. alvei, two...
In this study, we assess the scattering of light and auto-fluorescence from single bacterial cells to address challenge fast (<2 h), label-free phenotypic antimicrobial susceptibility testing (AST). Label-free flow cytometry is used for monitoring both respiration-related in two different fluorescence channels corresponding FAD NADH, morphological structural information contained scattered by individual bacteria during incubation with or without antibiotic. Large multi-parameter data are...
Timely microbiological results are essential to allow clinicians optimize the prescribed treatment, ideally at initial stage of therapeutic process. Several approaches have been proposed solve this issue and provide result in a few hours directly from sample such as molecular biology. However fast sensitive those methods not based on single phenotypic information which presents several drawbacks limitations. Optical advantage single-cell sensitivity probe phenotype measured cells. Here we...
Sample preparation is a key issue of modern analytical methods for in vitro diagnostics diseases with microbiological origins: to separate bacteria from other elements the complex biological samples are great importance. In present study, we investigated DEP force as way perform such de-complexification sample by extracting micro-organisms under highly non-uniform electric field micro-system based on an interdigitated electrodes array. Different parameters were optimize capture efficiency,...
A new colorimetric and easy-to-use solid sensor for the detection of indole-producing bacteria is described. The a nanoporous matrix with tailored pores doped reactant, DMACA. turns rapidly from transparent to green-blue when exposed metabolic indole coming cultures, both in liquid gas phases. An example discrimination between indole-positive (Eschericia coli) indole-negative (Hafnia alvei), two important contaminants food industry given.