A5004345012- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- MicroRNA in disease regulation
- Invertebrate Immune Response Mechanisms
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Plant Pathogens and Fungal Diseases
- Congenital heart defects research
- Congenital limb and hand anomalies
- Physiological and biochemical adaptations
- Neuroinflammation and Neurodegeneration Mechanisms
- Phytochemistry and Bioactive Compounds
- Cancer, Hypoxia, and Metabolism
- Neurobiology and Insect Physiology Research
- HIV Research and Treatment
- Autophagy in Disease and Therapy
- Congenital gastrointestinal and neural anomalies
- Cannabis and Cannabinoid Research
- Lung Cancer Research Studies
- Phytochemical compounds biological activities
- Neuroblastoma Research and Treatments
University of Bath
2007-2023
University of Bristol
2015-2020
University of Lisbon
2007
Abstract Neural crest cells are highly multipotent stem cells, but it remains unclear how their fate restriction to specific fates occurs. The direct model hypothesises that migrating maintain full multipotency, whilst progressive envisages fully transitioning partially-restricted intermediates before committing individual fates. Using zebrafish pigment cell development as a model, we show applying NanoString hybridization single transcriptional profiling and RNAscope in situ neural retain...
Abstract Background A major challenge lies in understanding the complexities of gene regulation. Mutation transcription factor SOX10 is associated with several human diseases. The disease phenotypes reflect function diverse tissues including neural crest, central nervous system and otic vesicle. As expected, expression pattern complex highly dynamic, but little known underlying mechanisms regulating its spatiotemporal pattern. conserved between all vertebrates characterised. Results We have...
Tissue damage leads to a robust and rapid inflammatory response whereby leukocytes are actively drawn toward the wound. Hydrogen peroxide (H2O2) has been shown be an immediate signal essential for recruitment of these blood cells wound sites in both Drosophila vertebrates [1Niethammer P. Grabher C. Look A.T. Mitchison T.J. A tissue-scale gradient hydrogen mediates detection zebrafish.Nature. 2009; 459: 996-999Crossref PubMed Scopus (1128) Google Scholar, 2Moreira S. Stramer B. Evans I. Wood...
Abstract Accurate lineage tracing is crucial to understanding of developmental and stem cell biology, but particularly challenging for transient highly dispersive cell‐types like the neural crest (NC). The authors report in this article a new zebrafish transgenic line Tg(‐4725sox10:Cre) ba74 . This expresses Cre under control well‐characterized portion sox10 promoter and, by crossing floxed‐reporter line, show that expression consistent with those described GFP reporter lines using same...
Drosophila provides a powerful model in which to study inflammation vivo, and previous studies have revealed many of the key signaling events critical for recruitment immune cells tissue damage. In fly, wounding stimulates rapid production hydrogen peroxide (H
Anaplastic lymphoma kinase (ALK) is an important drug target in many cancers, including lymphoma, neuroblastoma, and lung cancer. Here, we demonstrate proof-of-principle for a novel inexpensive assay ALK inhibitor activity identification zebrafish. We that the human oncogenic fusion, NPM-ALK, drives overproduction of iridophores, highly visible, shiny pigment cell-type Treatment with potent inhibitor, TAE684, fully inhibits production ALK-dependent iridophores. Using our assay, test multiple...
Abstract The yields of the essential oils isolated from aerial parts Pittosporum tobira grown in three different locations Lisbon, Portugal, were determined by hydrodistillation; chemical composition volatile oils, distillation–extraction, was analysed GC and GC–MS. In studied (leaves, flowers fruits), total 49 compounds identified. oil samples P. <0.05–0.17% volume/fresh weight (v/f.w.). n ‐Nonane most abundant component all (26–68%) myrcene (2–24%) second abundant. There no major...
Abstract Neural crest cells (NCCs) are highly multipotent stem cells. A long-standing controversy exists over the mechanism of NCC fate specification, specifically regarding presence and potency intermediate progenitors. The direct restriction (DFR) model, based on early in vivo clonal studies, hypothesised that intermediates absent migrating maintain full multipotency 1–6 . However, most authors favour progressive (PFR) models, with fully NCCs (ENCCs) transitioning to partially-restricted...