Abstract Interoceanic canals can facilitate biological invasions as they connect the world's oceans and remove dispersal barriers between bioregions. As a consequence, multiple opportunities for biotic exchange arise resulting establishment of migrant species often causes adverse ecological economic impacts. The Panama Canal is key region it connects Pacific Atlantic Oceans in Central America. In this study, we used two complementary methods (environmental DNA (eDNA) metabarcoding...

Abstract Land use is known to affect water quality yet the impact it has on aquatic microbial communities in tropical systems poorly understood. We used 16S metabarcoding assess of land bacterial column four streams central Panama. Each stream was influenced by a common Neotropical use: mature forest, secondary silvopasture and traditional cattle pasture. Bacterial community diversity composition were significantly nearby uses. Streams bordered forests had higher phylogenetic (Faith’s PD)...

Biofilms can methylate mercury (Hg) at higher rates than unattached bacteria and are increasingly recognized as important Hg methylation sites in the environment. Our previous study showed that biofilm cultures were up to 1 order of magnitude greater those planktonic a sulfate-reducing bacterium. To probe whether differential resulted from metabolic differences between these two cultures, assays following molybdate or chloroform inhibition (a specific inhibitor acetyl-CoA pathway) conducted...

Insects host a highly diverse microbiome, which plays crucial role in insect life. However, the composition and diversity of microbiomes associated with Neotropical freshwater insects is virtually unknown. In addition, extent to diversification this microbiome phylogenetic divergence remains be determined. Here, we present first comprehensive analysis bacterial communities six closely related species water striders Panama. We used comparative analyses assess associations between dominant...

Abstract Safe water storage is critical to preserve quality, especially when intermittent piped drinking supply creates a need for household storage. This study characterized practices and stored quality in 94 households (N = 94) among four peri-urban neighborhoods Arraiján, Panama with varying degrees of intermittency. We found that 18 (19.1%) unsafe containers. Forty-four (47%) samples had residual chlorine levels <0.2 mg/L. While 33 (35.1%) were positive total coliform bacteria,...

Monitoring of stream water quality is a key element resource management worldwide, but methods that are commonly used in temperate habitats may not be appropriate humid tropical systems. We assessed the influence four land uses on microbial 21 streams Panama Canal Watershed over one-year period, using common culture-based fecal indicator test and 16S rDNA metabarcoding. Each was located within one uses: mature forest, secondary silvopasture, traditional cattle pasture. Culturing detected...

Important changes in water bacterial communities were found across different stages of a drinking system with intermittent supply (IWS).

Intermittent water supply (IWS) is found in many parts of the world, especially low- and middle-income regions, characterized by a loss distribution system pressure, periods stagnation, intrusion contaminants, repressurization piped when service resumed after no-supply period, need for consumer storage. Although we have robust understanding impacts IWS on microbial quality, less research emphasis has been placed impact intermittency chemical such as formation disinfection byproducts (DBPs)....

Shipping corridors can be hotspots for biological invasions as they connect the world’s oceans and dissolve dispersal barriers between these aquatic systems. As a consequence, multiple opportunities biotic exchange arise resulting establishment of non-native species often causes adverse ecological economic impacts. In this study, combined effort traditional gillnetting eDNA-based surveys was implemented to characterize fish community Panama Canal, which is key region it connects Pacific...

Interoceanic canals can facilitate biological invasions as they connect the world’s oceans and dissolve dispersal barriers between bioregions. As a consequence, multiple opportunities for biotic exchange arise resulting establishment of migrant species often causes adverse ecological economic impacts. The Panama Canal is key region it connects Pacific Atlantic Oceans in Central America. In this study, we used two complementary methods (environmental DNA (eDNA) gillnetting) to survey fish...

Interoceanic canals can facilitate biological invasions as they connect the world’s oceans and dissolve dispersal barriers between bioregions. As a consequence, multiple opportunities for biotic exchange arise resulting establishment of migrant species often causes adverse ecological economic impacts. The Panama Canal is key region it connects Pacific Atlantic Oceans in Central America. In this study, we used two complementary methods (environmental DNA (eDNA) gillnetting) to survey fish...