A5081444911- Identification and Quantification in Food
- Marine Biology and Ecology Research
- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Isotope Analysis in Ecology
- Genomics and Phylogenetic Studies
- Marine and coastal ecosystems
- Marine Invertebrate Physiology and Ecology
- Marine and fisheries research
- Marine Ecology and Invasive Species
- Bacterial Identification and Susceptibility Testing
- Forensic and Genetic Research
- Ichthyology and Marine Biology
- Marine and coastal plant biology
- Coral and Marine Ecosystems Studies
- Ocean Acidification Effects and Responses
- Cephalopods and Marine Biology
- Aquaculture Nutrition and Growth
- Marine and environmental studies
- Crustacean biology and ecology
- Echinoderm biology and ecology
- Yersinia bacterium, plague, ectoparasites research
- Fish Ecology and Management Studies
- Marine Bivalve and Aquaculture Studies
- Data Analysis with R
Helmholtz Institute for Functional Marine Biodiversity
2018-2024
Carl von Ossietzky Universität Oldenburg
2018-2024
Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
2020-2024
Senckenberg am Meer
2012-2023
University of Bremen
2007-2021
Smithsonian Tropical Research Institute
2021
Leibniz Centre for Tropical Marine Research
2021
Abstract Characterization of species diversity zooplankton is key to understanding, assessing, and predicting the function future pelagic ecosystems throughout global ocean. The marine assemblage, including only metazoans, highly diverse taxonomically complex, with an estimated ~28,000 41 major taxonomic groups. This review provides a comprehensive summary DNA sequences for barcode region mitochondrial cytochrome oxidase I (COI) identified specimens. foundation this MetaZooGene Barcode Atlas...
During the last years DNA barcoding has become a popular method of choice for molecular specimen identification. Here we present comprehensive barcode library various crustacean taxa found in North Sea, one most extensively studied marine regions world. Our data set includes 1,332 barcodes covering 205 species, including Amphipoda, Copepoda, Decapoda, Isopoda, Thecostraca, and others. This dataset represents extensive Crustacea terms species number to date. By using Barcode Life Data Systems...
Valid fish species identification is an essential step both for fundamental science and fisheries management. The traditional mainly based on external morphological diagnostic characters, leading to inconsistent results in many cases. Here, we provide a sequence reference library mitochondrial cytochrome c oxidase subunit I (COI) valid of 93 North Atlantic originating from the Sea adjacent waters, including commercially exploited species. Neighbour-joining analysis K2P genetic distances...
Abstract Calanoid copepods play an important role in the pelagic ecosystem making them subject to various taxonomic and ecological studies, as well indicators for detecting changes marine habitat. For all these investigations, valid identification, mainly of sibling cryptic species early life history stages, represents a central issue. In this study, we compare identification methods calanoid copepod from North Sea adjacent regions total 333 specimens. Morphologically identified specimens...
Sequence-based specimen identification, known as DNA barcoding, is a common method complementing traditional morphology-based taxonomic assignments. The fundamental resource in barcoding the availability of taxonomically reliable sequence database to use reference for comparisons. Here, we provide library including 579 sequences mitochondrial cytochrome c oxidase subunit I 113 North Sea mollusc species. We tested efficacy this by simulating sequence-based identification scenario using Best...
We establish the new approach of environmental DNA (eDNA) analyses for North Sea. Our study uses a multigene approach, including mitochondrial cytochrome-c-oxidase subunit I (COI) gene analyzing species composition and nuclear hypervariable region V8 18S rDNA supraspecific biodiversity. A minibarcode primer (124 bp) was created on basis metazoan COI barcode library with 506 tested in silico, vitro, situ. applied high throughput sequencing to filtrates 23 near-bottom water samples taken at...
The applications of traditional morphological and molecular methods for species identification are greatly restricted by processing speed on a regional or greater scale generally considered unfeasible. In this context, high-throughput sequencing, metagenetics, has been proposed as an efficient tool to document biodiversity. Here we evaluated the effectiveness 454 pyrosequencing in marine metazoan community analysis using 18S rDNA: V1-V2 region. Multiplex region was used analyze two pooled...
Abstract Proteomic fingerprinting using MALDI-TOF mass spectrometry is a well-established tool for identifying microorganisms and has shown promising results identification of animal species, particularly disease vectors marine organisms. And thus can be vital biodiversity assessments in ecological studies. However, few studies have tested species across different orders classes. In this study, we collected data from 1246 specimens 198 to test diverse dataset. We also evaluated specimen...
Detecting fluctuations in the species composition of bloom-forming jellyfish requires ability to correctly identify each developmental stage. We verified diagnostic morphological and molecular genetic characters discriminate Cyanea lamarckii capillata from northern European waters. Intrusions subumbrellar muscle folds were present all C. >80 mm r-diameter (between opposite rhopalia tips), but absent lamarckii. Clearly visible wart-like papillae on central exumbrella >10–80 r-diameter,...
The euryhaline, eurytherm copepod Acartia tonsa plays a key role in many marine food webs as grazer on phytoplankton and prey species for commercially important planktivorous fishes. different populations of this cosmopolitan experience wide range environmental conditions. This study aims to elucidate the response A. changes multiple factors. effects temperature (T), salinity (S) interaction both variables recruitment processes were investigated show potential influences conditions its life...
Abstract Interoceanic canals can facilitate biological invasions as they connect the world's oceans and remove dispersal barriers between bioregions. As a consequence, multiple opportunities for biotic exchange arise resulting establishment of migrant species often causes adverse ecological economic impacts. The Panama Canal is key region it connects Pacific Atlantic Oceans in Central America. In this study, we used two complementary methods (environmental DNA (eDNA) metabarcoding...
Hydromedusae are widespread and diverse representatives of the gelatinous zooplankton, but often neglected because their inconspicuousness difficulties with identification. Here, we used an integrated approach combining both morphological molecular genetic analyses North Sea hydromedusae. Morphological identification was successfully carried out on living material, preservation in 4% formaldehyde allowed re-examination most features. Ethanol DESS were adequate fixatives for DNA led to...
Accurate species identification is crucial for ecological studies. For copepods, this usually based on a few diagnostic morphological characters, which can be highly conserved, resulting in an underestimation of diversity many copepod families. We elucidate richness the morphologically challenging and ecologically important deep-sea family Spinocalanidae tropical Atlantic by applying integrated taxonomic approach combining morphology, DNA-sequence analyses proteomic fingerprinting. In total,...
Taxonomic uncertainties in the morphological species identification and taxonomic revisions individual groups are known for all echinoderm classes. These discrimination have spawned application of molecular genetic techniques. However, as fundamental step to allow ensure future identification, valid comprehensive reference library entries comprising information together with various metadata essentially needed. In our study we compare techniques representatives North Sea classes, i.e....
Abstract Marine community diversity surveys require a reliable assessment to estimate ecosystem functions and their dynamics. For these, non‐invasive environmental DNA (eDNA) metabarcoding is increasingly applied in zoological studies complement or even replace traditional morphological identification methods. However, uncertainties remain about the accuracy of detected with eDNA capture actual field. Here, we validate reliability identifying metazoan biodiversity highly dynamic marine...
Isopods of the species Ligia oceanica are typical inhabitants rocky intertidal northern European coastline. The aim this study was to assess genetic structure using mitochondrial and nuclear sequence data. We analysed partial cytochrome c oxidase subunit I (CO1) 16S rRNA gene data 161 specimens collected from ten sites ranging Spain Norway. For selected specimens, we also sequenced hypervariable V7 expansion segment 18S as a supplementary marker. Furthermore, studied infection rate all by...
Abstract Brittle stars (Echinodermata: Ophiuroidea) comprise over 2, 000 species, all of which inhabit marine environments and can be abundant in the deep sea. Morphological plasticity number shape skeletal parts, as well variable colors, complicate correct species identification. Consequently, DNA sequence analysis play an important role In this study we compared genetic variability mitochondrial cytochrome c subunit I gene (COI) nuclear small ribosomal (SSU, 18S rDNA) to morphological...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 334:205-212 (2007) - doi:10.3354/meps334205 Comparative nutritional condition of larval dab Limanda limanda and lesser sandeel Ammodytes marinus in a highly variable environment Arne Michael Malzahn1,*, Catriona Clemmesen2, Karen Helen Wiltshire1, Silke Laakmann1,3, Maarten Boersma1,4 1Alfred Wegener...