A5041318703- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Gene Regulatory Network Analysis
- Cancer Genomics and Diagnostics
- Cytomegalovirus and herpesvirus research
- Plant tissue culture and regeneration
- Biochemical and Molecular Research
- Cancer survivorship and care
- Industrial Engineering and Technologies
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Plant Stress Responses and Tolerance
- Amino Acid Enzymes and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Biochemical Acid Research Studies
- Enzyme function and inhibition
- Meta-analysis and systematic reviews
- PARP inhibition in cancer therapy
- thermodynamics and calorimetric analyses
- Health Systems, Economic Evaluations, Quality of Life
- Photosynthetic Processes and Mechanisms
- Catalysis for Biomass Conversion
Nesher Technologies (United States)
2020-2023
University of California, Los Angeles
1968-2022
UCLA Health
2020
Significance With the recent discoveries of large reserves natural gas, efficient utilization one-carbon compounds for chemical synthesis would reduce raw material cost petroleum-based industry. Methanol is produced industrially from methane and a feedstock higher carbon compounds. However, current methanol requires high temperature pressure. Natural biological pathways are ATP inefficient. Here we constructed synthetic biocatalytic pathway that allows conversion to higher-chain alcohols or...
Stability in a metabolic system may not be obtained if incorrect amounts of enzymes are used. Without stability, some metabolites accumulate or deplete leading to the irreversible loss desired operating point. Even initial enzyme achieve stable steady state, changes amount due stochastic variations environmental move unstable region and lose steady-state quasi-steady-state flux. This situation is distinct from phenomenon characterized by typical sensitivity analysis, which focuses on smooth...
Health care research increasingly relies on assessment of data extracted from electronic medical records (EMRs). Clinical trial adverse event (AE) logs and patient-reported outcomes (PROs) are sources often available in the context specific projects. The aim this study was to evaluate extent concordance these sources.Patients enrolled clinical trials or receiving standard treatment for lung cancer (n = 62) completed validated questionnaires physical psychological symptoms at up three points....
Abstract An enzyme, catalyzing phosphoryl transfers from phosphoramidate or various hexose phosphates, has been purified to a high degree extracts of Escherichia coli. The purification involves the preferential denaturation contaminating proteins at acid and alkaline pH values, followed by chromatography on Biogel P-20, carboxymethyl cellulose, diethylaminoethyl cellulose. resulting enzyme is essentially homogeneous as shown acrylamide gel electrophoresis sucrose gradient centrifugation. It...
The product formation yield (product formed per unit substrate consumed) is often the most important performance indicator in metabolic engineering. Until now, actual cannot be predicted, but it can bounded by its maximum theoretical value. calculated considering stoichiometry of pathways and cofactor regeneration involved. Here we found that many cases, dynamic stability becomes an issue when excessive pathway flux drawn to a product. This constraint reduces renders maximal too loose...
Aim: There is an unmet need for predictive biomarkers immune checkpoint blockade in ovarian cancer. Homologous recombination deficiency (HRD) and immunoreactive molecular subtype may be associated with determinants of immunogenicity. Materials & methods: Neoantigen load, tumor inflammation signature (TIS), cell infiltrates individual checkpoints were assessed based on HRD status subtype. Results: Tumors demonstrated significantly higher expression neoantigens multiple check points, but not...
Abstract The stability of the Calvin Bassham Benson (CBB) cycle remains an area active computational research. Our understanding biology and prospect for bioengineered plants with higher productivity may both be impacted by a greater this area. Here we use ensemble modelling robustness analysis (EMRA) framework to show that action phosphate/G3P antiporter is much more significant maintenance than recently proposed G6P shunt. Additionally, interpret recent results suggesting overexpression...
Abstract The py_emra module implements the Ensemble Modeling Robustness Analysis (EMRA) algorithm in Python. Previous implementations MATLAB have been used to gain useful insights into metabolic dynamics and engineering strategies. package performs favorably comparison MATLAB. Potential extensions improvements are discussed.
Abstract Immune check point blockade (ICB) has recently transformed the treatment of non-small cell lung (NSCLC). However, majority patients do not respond to ICB. For ICB be effective, tumor neoantigens need presented in a human leukocyte antigen (HLA) class I-restricted manner. Recent studies by our group and others have shown that HLA-B44 supertype is associated with extended survival treated Herein, we hypothesize HLA-B44-specific motif neoepitopes generate an active immune...
3083 Background: Recent evidence suggests efficacy of immune checkpoint blockade may be influenced by human leukocyte antigen (HLA)-B. HLA-B27 supertype has an electronegative binding pocket which favorably binds and displays neoepitopes harboring positively charged amino acids (AAs). Based on surveillance, we postulate that B27 tumors have favorable should face negative selective pressure, with develop could more likely to upregulate escape mechanisms. Here evaluate the relationship between...
Abstract Purpose: Immune checkpoint blockade (ICB) has revolutionized treatment of melanoma and lung cancer. Recent evidence suggests that human leukocyte antigen (HLA) B supertype influences efficacy ICB. HLA-B27 B44 supertypes have electronegative electropositive binding pockets, respectively, preferentially bind display oppositely charged neoepitopes. We sought to evaluate the relationship between baseline immune activation neoepitopes in context HLA-B pockets. Methods: Datasets from a...