A5087864079- Intraperitoneal and Appendiceal Malignancies
- Cancer Genomics and Diagnostics
- Ovarian cancer diagnosis and treatment
- Colorectal Cancer Screening and Detection
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Cancer Cells and Metastasis
- RNA and protein synthesis mechanisms
- Gastrointestinal Tumor Research and Treatment
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- Genetic factors in colorectal cancer
- Neurobiology and Insect Physiology Research
- Colorectal Cancer Treatments and Studies
- Lymphoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Photoreceptor and optogenetics research
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Appendicitis Diagnosis and Management
- Enzyme Catalysis and Immobilization
The University of Texas MD Anderson Cancer Center
2019-2024
Northwell Health
2024
Columbia University Irving Medical Center
2016-2022
UCLA Medical Center
2021
University of California, Los Angeles
2020-2021
VA Greater Los Angeles Healthcare System
2021
UCLA Health
2020
Columbia University
2017
Center for Systems Biology
2014
Brandeis University
2009-2011
In recent years, a number of tools have become available for remotely activating neural circuits in Drosophila. Despite widespread and growing use, very little work has been done to characterize exactly how these affect activity identified fly neurons. Using the GAL4-UAS system, we expressed blue light–gated Channelrhodopsin-2 (ChR2) mutated form ChR2 (H134R-ChR2) motor sensory neurons Drosophila third-instar locomotor circuit. Neurons expressing H134R-ChR2 show enhanced responses light...
Glioma growth is driven by signaling that ultimately regulates protein synthesis. Gliomas are also complex at the cellular level and involve multiple cell types, including transformed reactive cells in brain tumor microenvironment. The distinct functions of various types likely lead to different requirements regulatory paradigms for Proneural gliomas can arise from transformation glial progenitors proliferate via mitogenic affects translation. To investigate translational regulation this...
Importance Appendiceal adenocarcinoma is a rare tumor, and given the inherent difficulties in performing prospective trials such disease, there are currently minimal high-quality data to guide treatment decisions, highlighting need for more preclinical clinical investigation this disease. Objective To prospectively evaluate effectiveness of fluoropyrimidine-based systemic chemotherapy patients with inoperable low-grade mucinous appendiceal adenocarcinoma. Design, Setting, Participants This...
Ribosome profiling has emerged as a powerful tool for genome-wide measurements of translation, but library construction requires multiple ligation steps and remains cumbersome relative to more conventional deep-sequencing experiments. We report new, ligation-free approach ribosome that does not require ligation. Library can be completed in one day with little 1 ng purified RNA footprints. apply mouse brain tissue identify new patterns cell type-specific translation test its ability...
Abstract Purpose: Appendiceal adenocarcinoma (AA) remains an orphan disease with limited treatment options for patients unable to undergo surgical resection. Evidence supporting the efficacy of combined VEGF and PD-1 inhibition in other tumor types provided a compelling rationale investigating this combination AA, where immune checkpoint inhibitors have not been explored previously. Experimental Design: We conducted prospective, single-arm phase II study evaluating safety atezolizumab...
<p>Supplementary Figures</p>
<div>AbstractPurpose:<p>Appendiceal adenocarcinoma is a rare malignancy with distinct histopathologic subtypes and natural history metastasis primarily limited to the peritoneum. Little known about molecular pathogenesis of appendiceal relative common tumors.</p>Experimental Design:<p>We analyzed data for patients within Guardant Health database appendix cancer (<i>n</i> = 718). We then identified at our institution (from October 2004–September 2022) whom...
<p>Appendiceal Adenocarcinoma Circulating Mutational Profiles</p>
Small bowel adenocarcinoma (SBA) is a rare malignancy marked with poor prognosis. The cellular and proteomic heterogeneity within the tumor immune microenvironment (TIME) of SBA likely driver prognosis, disease progression response to therapy. We have addressed major gap in knowledge TIME using highly multiplexed, protein imaging tumor-immune ecosystem generating comprehensive, single-cell level, spatial, atlas > 600,000 cells from 136 matched normal samples clinically genomically...
Here we incorporate recent advances in Drosophila neurogenetics and “optogenetics” into neuroscience laboratory exercises. We used the light-activated ion channel channelrhodopsin-2 (ChR2) tissue-specific genetic expression techniques to study neural basis of behavior larvae. designed implemented exercises using inexpensive, easy-to-use systems for delivering blue light pulses with fine temporal control. Students first examined behavioral effects activating glutamatergic neurons larvae then...
The Drosophila larval neuromuscular preparation has proven to be a useful tool for studying synaptic physiology1,2,3. Currently, the only means available evoke excitatory junctional potentials (EJPs) in this involves use of suction electrodes. In both research and teaching labs, students often have difficulty maneuvering manipulating type stimulating electrode. present work, we show how remotely stimulate at NMJ without By expressing channelrhodopsin2 (ChR2) 4,5,6 motor neurons using...
Abstract Cancer stem cells (CSC) play a critical role in metastasis, relapse, and therapy resistance colorectal cancer. While characterization of the normal lineage cell development intestine has led to identification many genes involved induction maintenance pluripotency, recent studies suggest significant heterogeneity CSC populations. Moreover, while canonical cancer marker have been identified, ability use these classical markers annotate stemness at single-cell level is limited. In this...
Abstract Protein synthesis is dysregulated in many diseases, but we lack a systems-level picture of how signaling molecules and RNA binding proteins interact with the translational machinery, largely due to technological limitations. Here present riboPLATE-seq, scalable method for generating paired libraries ribosome-associated total mRNA. As an extension PLATE-seq protocol, riboPLATE-seq utilizes barcoded primers pooled library preparation, additionally leverages anti-rRNA ribosome...
LettersApril 2021Nivolumab Treatment of Burkitt Lymphoma: A Case ReportB. Scott Colton, MD, Nicholas Hornstein, PhD, John Timmerman, Sarah Larson, and Glaspy, MPHB. MDUniversity California, Los Angeles, PhDUniversity MPHUniversity CaliforniaAuthor, Article, Disclosure Informationhttps://doi.org/10.7326/L20-1090 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail Background: lymphoma is an uncommon form that...
<div>AbstractPurpose:<p>Appendiceal adenocarcinoma (AA) remains an orphan disease with limited treatment options for patients unable to undergo surgical resection. Evidence supporting the efficacy of combined VEGF and PD-1 inhibition in other tumor types provided a compelling rationale investigating this combination AA, where immune checkpoint inhibitors have not been explored previously.</p>Experimental Design:<p>We conducted prospective, single-arm phase II study...
<p>Supplementary Figure 1: Consort Diagram Trial consort diagram</p>
<p>Supplementary Figure 2: Representative Pathology Images A,B: images from patient tumor biopsies large mucin deposits and minimal cellularity. Infiltrating lymphocytes are notable in both images.</p>
<p>Supplementary Figure 2: Representative Pathology Images A,B: images from patient tumor biopsies large mucin deposits and minimal cellularity. Infiltrating lymphocytes are notable in both images.</p>