A5044119346- Protein Interaction Studies and Fluorescence Analysis
- Drug Transport and Resistance Mechanisms
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Lanthanide and Transition Metal Complexes
- Proteins in Food Systems
- Pesticide Residue Analysis and Safety
- Antibiotics Pharmacokinetics and Efficacy
- Advanced Glycation End Products research
- NMR spectroscopy and applications
- Hemoglobin structure and function
- Protein purification and stability
- Probiotics and Fermented Foods
- Advanced Fluorescence Microscopy Techniques
- Nanoparticle-Based Drug Delivery
University of Opole
2021-2024
Medical University of Silesia
2017-2021
The aim of this study is to prepare a liposomal delivery system for 5-methyl-12 (H)-quino[3,4-b]-1,4-benzothiazine chloride (5-MBT) and the in vitro release characteristics. 5-MBT from complex with human serum albumin (HSA) [LDPPC/5-MBT]:HSA was examined using spectrophotometric method differential scanning calorimetry (DSC). Electronic paramagnetic resonance used assess influence pH environment on conformation phospholipids, latter determining degree encapsulated compound. applied...
Human serum albumin (HSA) plays a fundamental role in the human body. It takes part transport of exogenic and endogenic substances, especially drugs. Ibuprofen (IBU) is one most commonly used non-steroidal anti-inflammatory drugs, for pain relief, fever purposes. The binding ligands with HSA significant factor which determines toxicity therapeutic dosages these substances. aim this study was to compare degree ibuprofen at various temperatures protein solution pH values. In order evaluate...
Serum albumin is exposed to numerous structural modifications which affect its stability and activity. Glycation one of the processes leading loss original properties physiological function disorder. In terms long lasting states hyperglycemia, Advanced End-products (AGEs) are formed. AGEs responsible for cellular tissue structure damage that cause appearance a number health consequences premature aging. The aim present study was analyze conformational changes serum by...
Glycation process occurs in protein and becomes more pronounced diabetes when an increased amount of reducing sugar is present bloodstream. may cause conformational changes resulting the alterations its binding properties even though they occur at a distance from sites. The could be related to several pathological consequences such as diabetic nondiabetic cardiovascular diseases, cataract, renal dysfunction Alzheimer's disease. experiment was designed test impact glycation on sulfonylurea...
Human serum albumin (HSA) is the most abundant human plasma protein. HSA plays a crucial role in many binding endos- and exogenous substances, which affects their pharmacological effect. The innovative aspect of study not only interaction fatted defatted (dHSA) with ibuprofen (IBU), but analysis influence temperature on structural modifications between drug proteins from characteristic near hypothermia (308 K) to reflecting inflammation body (312 K 314 K). Ibuprofen non-steroidal...
Background: Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are one of the most commonly used groups medicinal compounds in world. The wide access to NSAIDs and various ways storing them due their easy accessibility often entail problem with stability durability resulting from exposure drugs external factors. aim research was evaluate vitro mechanism competition between ibuprofen (IBU) its degradation products, i.e., 4'-isobutylacetophenone (IBAP) (2RS)-2-(4- formylphenyl)propionic acid...
Metoprolol (MTP) is a cardio-selective β1-blocker used in hypertension, angina pectoris and chronic heart failure therapies. Serum albumin the most frequently occurring protein blood plasma. The binding of ligands to human serum (HSA) has an important effect on pharmacokinetics final clinical effects.The objectives this study included detailed analysis metoprolol - interactions low affinity sites, surface or within hydrophobic subdomain macromolecule, as well competition between MTP fatty...