A5041998912- Protein Interaction Studies and Fluorescence Analysis
- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- Drug Transport and Resistance Mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Surfactants and Colloidal Systems
- Porphyrin and Phthalocyanine Chemistry
- thermodynamics and calorimetric analyses
- Metal complexes synthesis and properties
- Analytical Chemistry and Chromatography
- Polymer crystallization and properties
- Protein purification and stability
- Spectroscopy and Quantum Chemical Studies
- Coagulation and Flocculation Studies
- Electron Spin Resonance Studies
- Polymer Surface Interaction Studies
- Metal-Organic Frameworks: Synthesis and Applications
- Synthesis and properties of polymers
- Epoxy Resin Curing Processes
- Lanthanide and Transition Metal Complexes
- Monoclonal and Polyclonal Antibodies Research
- Crystal structures of chemical compounds
- Field-Flow Fractionation Techniques
- RNA Interference and Gene Delivery
University of Opole
2020-2025
University of Silesia in Katowice
2008-2023
Medical University of Silesia
2006-2019
Centrum Badań Molekularnych i Makromolekularnych Polskiej Akademii Nauk
2009
Polish Academy of Sciences
2009
Background/Objectives: The principal aim of this work was to prepare a liposomal drug delivery system based on the commercial doxorubicin (DOX) and budding agent with promising anticancer activity, 9-(N-piperazinyl)-5-methyl-12(H)-quino [3,4-b][1,4]benzothiazinium chloride (9-PBThACl). Methods: A spectrophotometric methodology used meticulously investigate entrapment release characteristics new complexes (L) dipalmitoylphosphatidylcholine (DPPC) human serum albumin (HSA) its defeated analog...
Determining the properties of nanoparticles obtained by novel methods and defining scope their application as drug carriers has important practical significance. This article presents pioneering studies concerning high degree incorporation cytarabine (AraC) methotrexate (MTX) into liposome vesicles. The main focus this study were cytarabine-methotrexate-dipalmitoylphosphatidylcholine (DPPC) interactions observed in gel fluid phases DPPC bilayers. proposed new method use...
The aim of this study is to prepare a liposomal delivery system for 5-methyl-12 (H)-quino[3,4-b]-1,4-benzothiazine chloride (5-MBT) and the in vitro release characteristics. 5-MBT from complex with human serum albumin (HSA) [LDPPC/5-MBT]:HSA was examined using spectrophotometric method differential scanning calorimetry (DSC). Electronic paramagnetic resonance used assess influence pH environment on conformation phospholipids, latter determining degree encapsulated compound. applied...
Abstract Waste-expanded polystyrene (EPS) and virgin (VPS) were converted into ion exchangers by chemical modification. For this purpose, we used silica sulfuric acid as the sulfonating agent conducted sulfonation under heterogeneous conditions. VPS, influence of quantity agent, temperature, reaction time on product properties studied. As it appeared, simplifies in comparison with conventional methods. Fourier transform-infrared (FT-IR) spectra elemental analysis revealed that products...
Human serum albumin (HSA) plays a fundamental role in the human body. It takes part transport of exogenic and endogenic substances, especially drugs. Ibuprofen (IBU) is one most commonly used non-steroidal anti-inflammatory drugs, for pain relief, fever purposes. The binding ligands with HSA significant factor which determines toxicity therapeutic dosages these substances. aim this study was to compare degree ibuprofen at various temperatures protein solution pH values. In order evaluate...
Serum albumin is exposed to numerous structural modifications which affect its stability and activity. Glycation one of the processes leading loss original properties physiological function disorder. In terms long lasting states hyperglycemia, Advanced End-products (AGEs) are formed. AGEs responsible for cellular tissue structure damage that cause appearance a number health consequences premature aging. The aim present study was analyze conformational changes serum by...
Chemically functionalized graphene oxides could be used as novel drug carriers. Covalent alterations of lead to surface changes via formation chemical bonding while non-covalent ones involve van der Waals forces, hydrogen bonding, and π-π stacking interactions. modifications appear superior they can yield compounds with desired properties carriers prepared by other methods are less stable. Synthesis oxide-iminodiacetic acid oxide-glycine involves nucleophilic substitution oxide nanoparticles...
In this study waste polystyrene foam and virgin as a reference materials were converted by chemical reactions under heterogeneous conditions into useful products. Polymeric flocculants obtained from during the sulfonation with sulfuric acid sulfonating agent Ag2SO4 catalyst. The influence of temperature time reaction on properties products studied. successfully used in treatment water. Solid silica was also to convert polystyrene. comparison conventional methods simplifies makes easier...
The aim of this study is to encapsulate two drugs: 5-fluorouracil (5-FU) with the hydrophobic properties and 1-β-D-arabinofuranosylcytosine (Ara-C) amphiphilic into liposomes prepared by modified reverse-phase evaporation method (mREV) from L-α-phosphatidylcholine dipalmitoyl (DPPC). We studied thermotropic phase behavior liposome entrapped 5-FU Ara-C. It known that stability depends not only on chemical gradient loading, use membrane stabilizer such as sterols, but also transition...
Glycation process occurs in protein and becomes more pronounced diabetes when an increased amount of reducing sugar is present bloodstream. may cause conformational changes resulting the alterations its binding properties even though they occur at a distance from sites. The could be related to several pathological consequences such as diabetic nondiabetic cardiovascular diseases, cataract, renal dysfunction Alzheimer's disease. experiment was designed test impact glycation on sulfonylurea...
The effect of temperature on liposome structures has been investigated by means electron paramagnetic resonance spectroscopy with the use spin labelling technique. EPR spectra were recorded a Bruker EMX spectrometer at X band in range 300–340 K. Liposomes prepared from L?a?phosphatidylcholine dipalmitoyl (1,2?dihexadecanoyl?sn?glycerol?3?phosphocholine) 99% (DPPC), DL?a?phosphatidylcholine dimyristoyl (1,2?ditetradecanoyl?rac?glycerol?3?phosphocholine) (DMPC) and cholesterol...
Human serum albumin (HSA) is the most abundant human plasma protein. HSA plays a crucial role in many binding endos- and exogenous substances, which affects their pharmacological effect. The innovative aspect of study not only interaction fatted defatted (dHSA) with ibuprofen (IBU), but analysis influence temperature on structural modifications between drug proteins from characteristic near hypothermia (308 K) to reflecting inflammation body (312 K 314 K). Ibuprofen non-steroidal...