A5064579974- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Ion Channels and Receptors
- Electrolyte and hormonal disorders
- Magnesium in Health and Disease
- Genetic and Kidney Cyst Diseases
- Biomedical Research and Pathophysiology
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Erythrocyte Function and Pathophysiology
- Sodium Intake and Health
- Renal and related cancers
- Renal function and acid-base balance
- Receptor Mechanisms and Signaling
- Bipolar Disorder and Treatment
- Chemical and Physical Studies
- Dialysis and Renal Disease Management
- Organ Donation and Transplantation
- Cystic Fibrosis Research Advances
- Aldose Reductase and Taurine
- ATP Synthase and ATPases Research
- Magnetic and Electromagnetic Effects
- Thermoregulation and physiological responses
- Cardiomyopathy and Myosin Studies
- Island Studies and Pacific Affairs
The University of Texas Health Science Center at Houston
2015-2024
Lipetsk State Pedagogical University
2021
Institute of Cytology
2013-2017
RELX Group (United States)
2017
University of Tennessee Health Science Center
2017
Laboratoire d’immunologie intégrative du cancer
2015
Russian Academy of Sciences
2015
The thiazide-sensitive sodium chloride cotransporter (NCC) and the epithelial channel (ENaC) are two of most important determinants salt balance thus systemic blood pressure. Abnormalities in either result profound changes There is one segment nephron where these transporters coexpressed, second part distal convoluted tubule. This a key aldosterone-sensitive nephron, final regulator handling kidney. Aldosterone hormonal for both proteins. Despite shared regulators coexpression segment,...
Potassium Kir4.1/5.1 channels are abundantly expressed at the basolateral membrane of principal cells in cortical collecting duct (CCD), where they thought to modulate transport rates by controlling transepithelial voltage. Insulin and insulin-like growth factor-1 (IGF-1) stimulate apically localized epithelial sodium (ENaC) augment reabsorption CCD. However, little is known about their actions on potassium membrane. In this study, we implemented patch-clamp analysis freshly isolated murine...
Store-operated calcium entry (SOCE) is the mechanism by which extracellular signals elicit prolonged intracellular elevation to drive changes in fundamental cellular processes. Here, we investigated role of SOCE regulation renal water reabsorption, using inbred rat strain SHR-A3 as an animal model with disrupted SOCE. We found that SHR-A3, but not SHR-B2, have a novel truncating mutation gene encoding stromal interaction molecule 1 (STIM1), endoplasmic reticulum (Ca(2+)) sensor triggers...
Significance Gpr116 is an adhesion G protein-coupled receptor (aGPCR) that highly expressed in the kidney. Here, we report localizes to A-intercalated cells murine collecting duct, where it functions as a significant and critical regulator of renal acid secretion by regulating surface expression V-ATPase proton pumps. Kidney-specific knockout mice develop unique acid-base disorder, “renal tubular alkalosis,” characterized acidic urine alkaline blood. These findings have implications for...
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The renal collecting duct is continuously exposed to a wide spectrum of fluid flow rates and osmotic gradients. Expression mechanoactivated Piezo1 channel the most prominent in duct. However, status regulation functionally distinct principal intercalated cells (PCs ICs) remain be determined. We used pharmacological activation quantify Piezo1-mediated [Ca2+]i influx single-channel activity separately PCs ICs freshly isolated ducts with fluorescence imaging electrophysiological tools. also...
Autosomal-dominant polycystic kidney disease (ADPKD) is a devastating disorder that characterized by progressive decline in renal function as result of the development fluid-filled cysts. Defective flow-mediated [Ca2+]i responses and disrupted homeostasis have been repeatedly associated with cyst progression ADPKD. We previously demonstrated transient receptor potential vanilloid type 4 (TRPV4) channel imperative for murine distal tubule cells. To determine whether compromised TRPV4...
Since parathyroid hormone (PTH) is known to increase transient receptor potential vanilloid (TRPV)5 activity and decrease Na(+)-Cl(-) cotransporter (NCC) activity, we hypothesized that decreased NCC-mediated Na(+) reabsorption contributes the enhanced TRPV5 Ca(2+) seen with PTH. To test this, used mDCT15 cells expressing functional ruthenium red-sensitive (45)Ca(2+) uptake. PTH increased uptake 8.8 ± 0.7 nmol·mg(-1)·min(-1) (n = 4, P < 0.01) NCC from 75.4 2.7 20.3 1.3 0.01). Knockdown of Ras...
Abstract Mechanosensitive TRPV4 channel plays a dominant role in maintaining [Ca 2+ ] i homeostasis and flow‐sensitive signaling the renal tubule. Polycystic kidney disease (PKD) manifests as progressive cyst growth due to cAMP‐dependent fluid secretion along with deficient mechanosensitivity impaired activity. Here, we tested how regulation of function by dietary K + intake modulates rate cystogenesis mechanosensitive cystic cells PCK453 rats, homologous model human autosomal recessive PKD...
It is well-established that the kidney collecting duct (CD) plays a central role in regulation of systemic water homeostasis. Aquaporin 2 (AQP2)-dependent reabsorption CD critically depends on arginine vasopressin (AVP) antidiuretic input and presence favorable osmotic gradient at apical plasma membrane with tubular lumen being hypotonic compared to cytosol. This difference creates mechanical force leading an increase [Ca2+]i cells. The significance osmosensitive signaling for renal...
Plasma phosphate (Pi) levels are tightly controlled, and elevated plasma Pi associated with an increased risk of cardiovascular complications death. Two renal transport proteins mediate the majority reabsorption: Na+-phosphate cotransporters Npt2a Npt2c, accounting for 70-80% reabsorption. The aim present study was to determine in vitro effects a novel inhibitor (PF-06869206) opossum kidney (OK) cells as well its selectivity vivo knockout (Npt2a-/-) mice. In OK cells, caused dose-dependent...
Kidneys play a central role in regulation of potassium homeostasis and maintenance plasma K+ levels within narrow physiological range. With-no-lysine (WNK) kinases, specifically WNK1 WNK4, have been recognized to regulate balance, part, by orchestrating maxi channel (BK)-dependent secretion the aldosterone-sensitive distal nephron (ASDN), which includes connecting tubule collecting duct. We recently demonstrated that Ca2+-permeable TRPV4 is essential for BK activation ASDN. Furthermore, high...
ENaC‐mediated sodium reabsorption in the collecting duct (CD) is a critical determinant of urinary excretion. Existing evidence suggest direct stimulatory actions Angiotensin II (Ang II) on ENaC CD, independently aldosterone‐mineralocorticoid receptor (MR) signaling. Deletion major renal AT 1 isoform, 1a R, decreases blood pressure and reduces abundance despite elevated aldosterone levels. The mechanism this insufficient compensation not known. Here, we used patch clamp electrophysiology...
The renal collecting duct plays a critical role in setting urinary volume and composition, with principal cells transporting Na+ K+ intercalated mediating Cl− reabsorption. Published evidence implies Angiotensin II (Ang II) is potent regulator of the apical transport systems response to systemic depletion. However, virtually nothing known about Ang actions on basolateral conductance cells. Here, we combined macroscopic single channel patch clamp recordings from freshly isolated mouse ducts...
Abstract Bile acids, originally known to emulsify dietary lipids, are now established signalling molecules that regulate physiological processes. Signalling targets several proteins include the ion channels involved in regulating intestinal motility and bile viscosity. Studies show acids epithelial sodium channel (ENaC) cultured cell models heterologous expression systems. ENaC plays both local systemic roles extracellular fluids. Here we investigated whether expressed native tissues. We...
cAMP is a universal second messenger regulating plethora of processes in the kidney. Two downstream effectors are PKA and exchange protein directly activated by (Epac), which, unlike PKA, often linked to elevation [Ca2+]i. While both Epac isoforms (Epac1 Epac2) expressed along nephron, their relevance kidney remains obscure. We combined ratiometric calcium imaging with quantitative immunoblotting, immunofluorescent confocal microscopy, balance studies mice lacking Epac1 or Epac2 determine...
Exchange proteins directly activated by cAMP (Epacs) are abundantly expressed in the renal tubules. We used genetic and pharmacological tools combination with balance, electrophysiological, biochemical approaches to examine role of Epac1 Epac2 sodium handling. demonstrate that Epac1-/- Epac2-/- mice exhibit a delayed anti-natriuresis dietary restriction despite augmented aldosterone levels. This was associated significantly lower response epithelial Na+ channel (ENaC) blocker amiloride,...
Tight regulation of K+ balance is fundamental for normal physiology. Reduced dietary intake, which common in Western diets, often leads to hypokalemia and associated cardiovascular- kidney-related pathologies. The distal nephron, and, specifically, the collecting duct (CD), major site controlled reabsorption via H+-K+-ATPase state deficiency. We (Mamenko MV, Boukelmoune N, Tomilin VN, Zaika OL, Jensen VB, O'Neil RG, Pochynyuk OM. Kidney Int 91: 1398-1409, 2017) have previously demonstrated...
The collecting duct is a highly adaptive terminal part of the nephron, which essential for maintaining systemic homeostasis. Principal and intercalated cells perform different physiological tasks exhibit distinctive morphology. However, acid-secreting A- base secreting B-type cannot be easily separated in functional studies. We used BCECF-sensitive intracellular pH (pHi) measurements split-opened ducts followed by immunofluorescent microscopy WT cell-specific ClC-K2−/− mice to demonstrate...