Three-dimensional (3D) spheroids of mesenchymal stem cells (MSCs) have high biological significance due to more accurate imitation physiological conditions, in vivo processes and greater therapeutic efficiency compared monolayer MSCs. However, molecular specific mechanisms regulating the effectiveness remain unknown. Endometrial MSCs are considered as reliable potent objects for cell-based therapy availability minor ethical concerns. Thus, knowledge controlling eMSC is clinical importance....

Mesenchymal stem cells obtained from desquamated endometrium (eMSCs) are considered as reliable and promising objects for cell-based therapy. eMSCs aggregated into three-dimensional (3D) spheroids demonstrate greater efficiency compared to monolayer 2D eMSCs. However, molecular processes specific mechanisms regulating the effectiveness of remain unknown. Regulation a number physiological reactions in MSCs is associated with functioning Ca2+-permeable mechanosensitive Piezo1 channels. In our...

Calcium-activated potassium channels (KCa) are important participants in calcium signaling pathways due to their ability be activated by an increase intracellular free concentration. KCa involved the regulation of cellular processes both normal and pathophysiological conditions, including oncotransformation. Previously, using patch-clamp, we registered currents plasma membrane human chronic myeloid leukemia K562 cells, whose activity was controlled local Ca2+ entry via mechanosensitive...

Endometrial mesenchymal stem cells (eMSCs) are a specific class of stromal which have the capability to migrate, develop and differentiate into different types such as adipocytes, osteocytes or chondrocytes. It is this unique plasticity that makes eMSCs significant for cellular therapy regenerative medicine. Stem choose their way development by analyzing extracellular intracellular signals generated mechanical force from microenvironment. Mechanosensitive channels part toolkit feels...

Piezo1 is a Ca

Abstract Membrane cholesterol and lipid rafts are implicated in various signalling processes involving actin rearrangement living cells. However, functional link between raft integrity organisation of cytoskeleton remains unclear. We have compared the effect sequestration on F‐actin structures normal transformed fibroblasts which microfilament system is developed to a different extent. The depletion membrane by methyl‐beta‐cyclodextrin (MbCD) resulted disruption plasma as it was revealed...

Lung cancer is one of the most common types in world. Despite existing treatment strategies, overall patient survival remains low and new targeted therapies are required. Acidification tumor microenvironment drives growth metastasis many cancers. Acid sensors such as acid-sensing ion channels (ASICs) may become promising targets for lung therapy. Previously, we showed that inhibition ASIC1 by a recombinant analogue mambalgin-2 from Dendroaspis polylepis controls oncogenic processes leukemia,...

Abstract The study of ion channels in stem cells provides important information about their role cell fate. Previously we have identified the activity calcium-activated potassium big conductance (BK channels) human endometrium-derived mesenchymal (eMSCs). BK could significant impact into signaling processes by modulating membrane potential. potential and ionic permeability dynamically changes during cycle transitions. Here, aimed at verification as transporting pathway regulating passageway...

The actin cytoskeleton is indispensable for the motility and migration of all types cells; therefore, it plays a crucial role in ability tissues to repair. Mesenchymal stem cells are intensively used regenerative medicine, but usually relatively low percent transplanted reaches injury. To overcome this evident limitation, researchers try enhance rate cells. As one approaches, co-cultivation preconditioning with biologically active compounds, which can cause rearrangements followed by an...

Piezo1/2 are mechanosensitive calcium-permeable channels that can be activated by various modes of membrane deformation. The identification the small molecule Yoda1, a synthetic Piezo1 agonist, revealed possibility chemical activation channel. Stimulating effects Yoda1 on have been mainly documented using over-expressing cellular systems or channel proteins incorporated in artificial lipid bilayers. However, activating effect native plasma living cells remains generally undefined, despite...

Statins are the most commonly prescribed agents used to modulate cholesterol levels in course of hypercholesterolemia treatment because their relative tolerability and LDL-C lowering effect. Recently, there emerging interests perspectives statin drugs as anticancer based on preclinical evidence antiproliferative, proapoptotic, anti-invasive properties. Functional impact application transformed cells still remains obscure that requires systematic study adequate cellular models provide correct...

Sodium influx is tightly regulated in the cells of blood origin. Amiloride-insensitive sodium channels were identified as one main sodium-transporting pathways leukemia cells. To date, all known regulatory these are coupled with intracellular actin cytoskeleton dynamics. Here, to search for physiological mechanisms controlling epithelial Na+ channel (ENaC)-like channels, we utilized K562 a unique model examine single behavior whole-cell patch-clamp experiments. We have shown first time that...

Melanoma is a highly aggressive type of skin cancer produced through the malignant transformation melanocytes, and it usually associated with poor prognosis. Clinically, melanoma has several stages migration invasion cells skin's layers, rapid spreading formation tumors in multiple organs. The main problem emergence resistance to applied methods treatment; thus, primary importance find more crucial signaling pathways that control progression this could be targeted prevent spreading. Here, we...