A5062880626- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Infant Nutrition and Health
- Biotin and Related Studies
- Neonatal Respiratory Health Research
- bioluminescence and chemiluminescence research
- Nicotinic Acetylcholine Receptors Study
- Antimicrobial Peptides and Activities
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- Click Chemistry and Applications
- Child and Adolescent Psychosocial and Emotional Development
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Fungal and yeast genetics research
- Suicide and Self-Harm Studies
- Retinal Development and Disorders
- Analytical Chemistry and Sensors
- Grief, Bereavement, and Mental Health
- Mass Spectrometry Techniques and Applications
University of Michigan
2020-2025
Washtenaw Community College
2021
Gordon College
2016-2018
Gordon State College
2016
Gordon-Conwell Theological Seminary
2016
Genetically encoded fluorescent sensors have been widely applied to detect cell signaling molecules and events.
G protein-coupled receptors (GPCRs) transduce the effects of many neuromodulators including dopamine, serotonin, epinephrine, acetylcholine, and opioids. The localization synthetic or endogenous GPCR agonists impacts their action on specific neuronal pathways. In this paper, we show a series single-protein chain integrator sensors that are highly modular could potentially be used to determine agonist across brain. We previously engineered for mu- kappa-opioid receptor called M-...
Abstract Mu‐opioid receptor (MOR) signaling regulates multiple neuronal pathways, including those involved in pain, reward, and respiration. To advance the understanding of MOR's roles pain modulation, there is a need for high‐throughput screening methods opioids vitro high‐resolution mapping brain. fill this need, we designed characterized genetically encoded fluorescent reporter, called S ingle‐chain P rotein‐based O pioid T ransmission I ndicator ool M OR (M‐SPOTIT). M‐SPOTIT represents...
In Brief Adolescent depression is a serious problem affecting 10.7% of all teens and 29.9% high school students; 17% students have contemplated suicide. Yet, in often unrecognized. This article relays the tragic death 17-year-old, along with symptoms suicide adolescents; DSM-5 criteria for depression; treatments including protective factors, psychotherapy, medications; imparts interventions addressing this huge but silent crisis. How adolescent depression? Around 11% ages 12–17 30% suffered...
The mu-opioid receptor (MOR) regulates the neuronal pathways involved in pain, reward, and respiration. To increase our understanding of MOR's roles these pathways, there is a need to detect opioids at cellular resolution. Here, we engineered an improved opioid-sensor, called M-SPOTIT2, which 11x brighter than previously M-SPOTIT1.1. We M-SPOTIT2 by adding amino acids YNSH, located near fluorophore enhanced green fluorescent protein, circular permuted protein M-SPOTIT2. M-SPOTIT1.1 HEK293T...
Modular fluorescent sensor motifs are needed to design sensors for detecting various cellular processes and functional molecules. Here, we took advantage of the versatility a new motif series called SPOTon. SPOTon integrate signal from either opioids, protein–protein interactions, or protease activities generate persistent green fluorescence. We demonstrate that can be engineered with temporal gating allow detection these events during user-defined time window, providing information about...
Abstract Protein‐protein interactions (PPIs) are ubiquitously involved in cellular processes such as gene expression, enzymatic catalysis, and signal transduction. To study dynamic PPIs, real‐time methods Förster resonance energy transfer bioluminescence can provide high temporal resolution, but they only allow PPI detection a limited area at time do not permit post‐PPI analysis or manipulation of the cells. Integration yeast two‐hybrid system split protein systems integrate signals over...
Abstract GPCRs transduce the effects of many neuromodulators including dopamine, serotonin, epinephrine, acetylcholine, and opioids. The localization synthetic or endogenous GPCR agonists impacts their action on specific neuronal pathways. In this paper, we show a series single-protein chain integrator sensors to determine agonist in whole brain. We previously engineered for mu kappa opioid receptor called M- K-SPOTIT, respectively. Here, new sensor design platform SPOTall that used engineer...
Abstract Mu‐opioid receptor (MOR) signaling regulates multiple neuronal pathways, including those involved in pain, reward, and respiration. To advance the understanding of MOR's roles pain modulation, there is a need for high‐throughput screening methods opioids vitro high‐resolution mapping brain. fill this need, we designed characterized genetically encoded fluorescent reporter, called S ingle‐chain P rotein‐based O pioid T ransmission I ndicator ool M OR (M‐SPOTIT). M‐SPOTIT represents...
Eine neuartige Klasse von genetisch kodierten Fluoreszenzsensoren zum Nachweis Opioid-Molekülen stellen Kayla E. Kroning und Wenjing Wang in ihrem Forschungsartikel auf S. 13470 vor. Diese Opioid-Sensoren werden SPOTIT (single-chain protein-based opioid transmission indicator tool) genannt. Bei der Erkennung Opioiden wird aktiviert erzeugt eine persistente grüne Fluoreszenzmarkierung für die Bildanalyse. könnte das Hochdurchsatz-Screening Zellkulturen den In-vivo-Nachweis zellulärer...