A5037661740- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- Neurological disorders and treatments
- Advanced biosensing and bioanalysis techniques
- Parkinson's Disease Mechanisms and Treatments
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Botulinum Toxin and Related Neurological Disorders
- Chemical Synthesis and Analysis
- Receptor Mechanisms and Signaling
- Fungal and yeast genetics research
University of Michigan
2021-2022
Ann Arbor Center for Independent Living
2021
A circularly permuted AsLOV2 domain has been designed to photocontrol peptides by caging their C-terminus. This photoswitch provides a new module for engineering optogenetic tools and general approach lower the background of LOV-based tools.
Abstract Protein‐protein interactions (PPIs) are ubiquitously involved in cellular processes such as gene expression, enzymatic catalysis, and signal transduction. To study dynamic PPIs, real‐time methods Förster resonance energy transfer bioluminescence can provide high temporal resolution, but they only allow PPI detection a limited area at time do not permit post‐PPI analysis or manipulation of the cells. Integration yeast two‐hybrid system split protein systems integrate signals over...
Understanding the regulation of α-synuclein release could be important in better understanding Parkinson's disease development, progression, and treatment. Advances such studies are hindered by technical challenges that limit ability to monitor concentration vivo. We developed a novel microdialysis method coupled with specific sensitive immunoassay requires small sample volume (1 μL). Using this method, basal level was estimated at 254 ± 78 pM striatum freely moving mice. Additionally, we...
Abstract We re-engineered a commonly-used light-sensing protein, LOV domain, using circular permutation strategy to allow photoswitchable control of the C-terminus peptide. demonstrate that use circularly permuted domain on its own or together with original could expand engineering capabilities optogenetic tools.