A5001565953- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- ATP Synthase and ATPases Research
- Membrane-based Ion Separation Techniques
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Electrochemical Analysis and Applications
- Cardiac electrophysiology and arrhythmias
- Analytical Chemistry and Sensors
- Molecular Sensors and Ion Detection
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Mass Spectrometry Techniques and Applications
- Hydrogen Storage and Materials
- Bacterial Genetics and Biotechnology
- Magnetic and Electromagnetic Effects
- Drug Transport and Resistance Mechanisms
- Fuel Cells and Related Materials
- Force Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Advanced Thermodynamics and Statistical Mechanics
University of Konstanz
2011-2021
Laboratoire de Chimie Physique
2008
Université Paris-Sud
2008
The University of Sydney
2002-2008
Centre National de la Recherche Scientifique
2006-2008
Sorbonne Université
2006
Institut de Biologie Physico-Chimique
2006
Biologie du Chloroplaste et Perception de la Lumière chez les Microalgues
2006
Max Planck Institute of Biophysics
2002
Weizmann Institute of Science
1994-2000
Experiments were designed to characterize several partial reactions of the Na,K-ATPase and demonstrate that a model can be defined reproduces most transport features pump with single set kientic parameters. We used fluorescence label 5-iodoacetamidofluorescein, which is thought sensitive conformational changes, styryl dye RH 421, applied detect ion-binding -release reactions. In addition transient electric currents measured, are associated mainly E1-->E2 transition. Numerical...
Measurements with different chemically modified gramicidins in lipid bilayer membranes were used to discriminate between the dimeric pi(L,D) helix proposed by Urry and parallel or antiparallel helices Veatch Blout. Evidence for was obtained on basis of actions a negatively charged O-pyromellitylgramicidin N-pyromellityldesformylgramicidin membranes. O-Pyromellitylgramicidin forms ionic channels when it is applied both sides membrane. In contrast unmodified gramicidin, inactive only one side...
Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity difficulty in overproducing them native form. MPs can be overexpressed non-native state as inclusion bodies, but inducing achieve functional three-dimensional structure has proven a challenge. We describe here use an amphipathic polymer, amphipol A8-35, novel environment that allows both β-barrel α-helical fold state, absence detergents or lipids. Amphipols,...
Since the beginning of investigations Na,K-ATPase, it has been well-known that Mg2+ is an essential cofactor for activation enzymatic ATP hydrolysis without being transported through cell membrane. Moreover, experimental evidence collected years shows ions have a regulatory effect on ion transport by interacting with cytoplasmic side pump. Our experiments allowed us to reveal underlying mechanism. able bind site outside membrane domain protein's α subunit, close entrance access channel...
Alveolar liquid clearance is regulated by Na(+) uptake through the apically expressed epithelial sodium channel (ENaC) and basolaterally localized Na(+)-K(+)-ATPase in type II alveolar cells. Dysfunction of these transporters during pulmonary inflammation can contribute to edema.In this study, we sought determine precise mechanism which TIP peptide, mimicking lectin-like domain tumor necrosis factor (TNF), stimulates a homologous cell system presence or absence bacterial toxin pneumolysin...