A5017868950- Nanoplatforms for cancer theranostics
- Fullerene Chemistry and Applications
- Carbon Nanotubes in Composites
- Photodynamic Therapy Research Studies
- Nanocluster Synthesis and Applications
- Graphene research and applications
- Luminescence and Fluorescent Materials
- Gold and Silver Nanoparticles Synthesis and Applications
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Porphyrin and Phthalocyanine Chemistry
- Graphene and Nanomaterials Applications
- Diamond and Carbon-based Materials Research
- Advanced Nanomaterials in Catalysis
- Carbon and Quantum Dots Applications
- Molecular Sensors and Ion Detection
- Molecular Junctions and Nanostructures
- Nanopore and Nanochannel Transport Studies
- Quantum Dots Synthesis And Properties
- Photochromic and Fluorescence Chemistry
- Supramolecular Self-Assembly in Materials
- Mass Spectrometry Techniques and Applications
- Context-Aware Activity Recognition Systems
- Conducting polymers and applications
- Synthesis and Catalytic Reactions
University of Bologna
2015-2024
Istituto di Genetica Molecolare
2023
Istituto Ortopedico Rizzoli
2023
Rzeszów University
2023
Fluorescence bioimaging is a non‐invasive technique that permits to investigate living organism in real time with high tridimensional resolution. Properly engineered fluorescent (or photoluminescent) nanoparticles promise surpass conventional molecular probes as contrast agent. Photoluminescent semiconductor quantum dots show, for example, enhanced brightness and photostability. Concerns arising from the toxic metal content of prompted search alternative inorganic similar properties but less...
Photodynamic therapy (PDT) represents a promising therapeutic modality for cancer. Here we used an orthogonal nanoarchitectonics approach (genetic/chemical) to engineer M13 bacteriophages as targeted vectors efficient photodynamic killing of cancer cells. was genetically refactored display on the phage tip peptide (SYPIPDT) able bind epidermal growth factor receptor (EGFR). The M13EGFR phages demonstrated EGFR-targeted tropism and were internalized by A431 cells, that overexpress EGFR. Using...
Abstract Photodynamic therapy (PDT) represents an emerging strategy to treat various malignancies, including colorectal cancer (CC), the third most common type. This work presents engineered M13 phage retargeted towards CC cells through pentavalent display of a disulfide-constrained peptide nonamer. The nanovector was conjugated with photosensitizer Rose Bengal (RB), and photodynamic anticancer effects resulting -RB bioconjugate were investigated on cells. We show that upon irradiation is...
Non-fluorescent nanoparticles resulting from the self-assembly of a new perylene diimide behave as fluorogenic probes for biological cells under physiological conditions giving dosage-dependent green or red fluorescence and showing very low cytotoxicity. The emission colour can be tuned by photo-irradiation to achieve multicolour labelling.
Abstract Photo‐switching of the NIR emission gold nanoparticles (GNP) upon photo‐isomerization azobenzene ligands, bound to surface, is demonstrated. Photophysical results confirm occurrence an excitation energy transfer process from ligands GNP that produces sensitized emission. Because this process, efficiency core, much higher for trans form than cis one, and t → c causes a relevant decrease As consequence, can be monitored by ratiometric detection dual excitation. The was followed in...
Abstract Fullerenes are candidates for theranostic applications because of their high photodynamic activity and intrinsic multimodal imaging contrast. However, fullerenes suffer from low solubility in aqueous media, poor biocompatibility, cell toxicity, a tendency to aggregate. C 70 @lysozyme is introduced herein as novel bioconjugate that harmless cellular environment, yet also photoactive has excellent optical optoacoustic contrast tracking uptake intracellular localization. The formation,...
Photodynamic therapy (PDT) is a potential synergistic approach to chemotherapy for treating ovarian cancer, the most lethal gynecologic malignancy. Here we used M13 bacteriophage as targeted vector efficient photodynamic killing of SKOV3 and COV362 cells. The phage was refactored (M13r) display an EGFR binding peptide in its tip that frequently overexpressed cancer. conjugated with chlorin e6 (Ce6), one widely photosensitizers (M13r-Ce6). new platform, upon irradiation, generated ROS by type...
C60@lysozyme showed significant visible light-induced singlet oxygen generation in water, indicating the potential of this hybrid as an agent for photodynamic therapy. The reactive species (ROS) concentration generated by during irradiation depends on light source, time and hybrid. significantly reduced HeLa cell viability response to irradiation. H2O2, due photoactivity C60@lysozyme, causes death via easy permeation hydrogen peroxide through membrane activation endogenous ROS production.
Lysozyme is used as a host molecule to disperse monomolecularly C<sub>60</sub> in water preserving the properties.
Fluorescence is a powerful tool for mapping biological events in real-time with high spatial resolution. Ultra-bright probes are needed order to achieve sensitivity: these typically obtained by gathering huge number of fluorophores single nanoparticle (NP). Unfortunately this assembly produces quenching the fluorescence because short-range intermolecular interactions. Here we demonstrate that rational structural modification well-known molecular fluorophore...
The high hydrophobicity of fullerenes and the resulting formation aggregates in aqueous solutions hamper possibility their exploitation many technological applications. Noncovalent bioconjugation with proteins is an emerging approach for dispersion media. Contrary to covalent functionalization, preserves physicochemical properties carbon nanostructure. unique photophysical photochemical are then fully accessible applications nanomedicine, sensoristic, biocatalysis materials science fields....
The electronic, optical, and redox properties of thiophene-based materials have made them pivotal in nanoscience nanotechnology. However, the exploitation oligothiophenes photodynamic therapy is hindered by their intrinsic hydrophobicity that lowers biocompatibility availability water environments. Here, we developed human serum albumin (HSA)–oligothiophene bioconjugates afford use insoluble physiological UV–vis electrophoresis proved conjugation oligothiophene sensitizers to protein....
Here, we show that in thiophene-based core@shell nanoparticles, namely, P3HT@PTDO NPs, the nanosegregation of materials results a peculiar photoreactivity, which, together with their soft and biocompatible nature, makes them interesting bioplatforms. By combining macroscopic microscopic Kelvin probe measurements, surface NPs becomes rich negative charges under light illumination─due to promotion photogenerated electrons from inner P3HT core outer oxidized PTDO shell─making more reactive...
Growing antibiotic resistance has encouraged the revival of phage-inspired antimicrobial approaches. On other hand, photodynamic therapy (PDT) is considered a very promising research domain for protection against infectious diseases. Yet, few efforts have been made to combine advantages both approaches in modular, retargetable platform. Here, we foster M13 bacteriophage as multifunctional scaffold, enabling selective killing bacteria. We took advantage well-defined molecular biology...
The lack of solubility in water and the formation aggregates hamper many opportunities for technological exploitation C60. Here, different peptides were designed synthesized with aim monomolecular dispersion C60 water. Phenylalanines used as recognizing moieties, able to interact through π–π stacking, while a varying number glycines spacers, connect two terminal phenylalanines. best performance was obtained FGGGF peptidic nanotweezer at pH 12. A full characterization this adduct carried out....
Fullerenes are considered excellent photosensitizers, being highly suitable for photodynamic therapy (PDT). A lack of water solubility and low biocompatibility are, in many instances, still hampering the full exploitation their potential nanomedicine. Here, we used human serum albumin (HSA) to disperse fullerenes by binding up five fullerene cages inside hydrophobic cavities. Albumin was bioconjugated with folic acid specifically address folate receptors that usually overexpressed several...
Abstract A general single‐step approach is introduced for the green fabrication of hybrid biosensors from water dispersion. The resulting device integrates semiconducting properties a carbon nanotube (CNT) and functionality protein. In initial aqueous phase, protein (viz., lysozyme [LZ]) disperses (6,5)CNT. Drop‐casting dispersion on test pattern (a silicon wafer with interdigitated Au source drain electrodes) yields fully operating, robust, electrolyte‐gated transistor (EGT) in one step....