A5066997677- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Diet, Metabolism, and Disease
- Free Radicals and Antioxidants
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Antioxidant Activity and Oxidative Stress
- Environmental Toxicology and Ecotoxicology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Drug Transport and Resistance Mechanisms
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- High Altitude and Hypoxia
- Bioactive Compounds and Antitumor Agents
- Autophagy in Disease and Therapy
- Pesticide and Herbicide Environmental Studies
- Electron Spin Resonance Studies
- Dietary Effects on Health
University of Łódź
2016-2025
Baker Heart and Diabetes Institute
2009-2014
The Alfred Hospital
2009-2014
The University of Melbourne
2012
Monash University
2012
Charles University
2010
University of Vienna
2010
OBJECTIVE Results from the Diabetes Control Complications Trial (DCCT) and subsequent Epidemiology of Interventions (EDIC) Study more recently U.K. Prospective (UKPDS) have revealed that deleterious end-organ effects occurred in both conventional aggressively treated subjects continued to operate >5 years after patients had returned usual glycemic control is interpreted as a legacy past glycemia known “hyperglycemic memory.” We hypothesized transient hyperglycemia mediates persistent...
Abstract Butyrate and R-β-hydroxybutyrate are two related short chain fatty acids naturally found in mammals. Butyrate, produced by enteric butyric bacteria, is present at millimolar concentrations the gastrointestinal tract lower levels blood; R-β-hydroxybutyrate, main ketone body, liver during fasting can reach circulation. Both molecules have been shown to be histone deacetylase (HDAC) inhibitors, their administration has associated an improved metabolic profile better cellular oxidative...
Quercetin, a common plant polyphenol, has been reported to show both antioxidant and prooxidant properties. We studied the effects of quercetin on A549 cells in vitro culture. found that low concentrations flavonoid stimulated cell proliferation increased total capacity (TAC) cells; while higher decreased survival viability, thiol content, TAC activities superoxide dismutase, catalase glutathione S-transferase. Quercetin production reactive oxygen species but produced peroxides medium. The...
Emerging evidence suggests that poor glycemic control mediates post-translational modifications to the H3 histone tail. We are only beginning understand dynamic role of some diverse epigenetic changes mediated by hyperglycemia at single loci, yet elevated glucose levels thought regulate genome-wide changes, and this still remains poorly understood. In article we describe H3K9/K14 hyperacetylation DNA methylation maps conferred in primary human vascular cells. Chromatin immunoprecipitation...
Rationale: Epigenetic changes are implicated in the persisting vascular effects of hyperglycemia. The precise mechanism whereby chromatin structure and subsequent gene expression regulated by glucose endothelial cells remain to be fully defined. Objective: We have studied molecular functional Set7 methyltransferase associates with formation histone methylation response current previous exposure glucose. Methods Results: To characterize identity protein, we used overexpressing or lacking...
Heme oxygenase-1 (HO-1) is an antioxidative, antiinflammatory, and cytoprotective enzyme that induced in response to cellular stress. The HO-1 promoter contains a (GT)n microsatellite DNA, the number of GT repeats can influence occurrence cardiovascular diseases. We elucidated effect this polymorphism on endothelial cells isolated from newborns different genotypes.On basis expression, we classified alleles into 3 groups: short (S) (most active, < or = 23), medium (moderately GT=24 28), long...
HDAC inhibitors can regulate gene expression by post-translational modification of histone as well nonhistone proteins. Often studied at single loci, increased acetylation is the paradigmatic mechanism action. However, little known extent genome-wide changes in cells stimulated hydroxamic acids, TSA and SAHA. In this article, we map vascular chromatin modifications including H3 lysine 9 14 (H3K9/14ac) using immunoprecipitation (ChIP) coupled with massive parallel sequencing (ChIP-seq). Since...
The ketogenic diet (KD), characterized by very limited dietary carbohydrate intake and used as nutritional treatment for GLUT1-deficiency syndromes pharmacologically refractory epilepsy, may promote weight loss improve metabolic fitness, potentially alleviating the symptoms of osteoarthritis. Here, we have studied effects administration a in mice previously rendered obese feeding high fat (HFD) submitted to surgical destabilization medial meniscus mimic osteoarthritis.6-weeks old were fed an...
Epigallocatechin gallate (EGCG), the main green tea polyphenol, exerts a wide variety of biological actions. Epigenetically, catechin has been classified as DNMTs inhibitor, however, its impact on histone modifications and chromatin structure is still poorly understood. The purpose this study was to find EGCG posttranslational machinery remodeling in human endothelial cells both microvascular (HMEC-1) vein (HUVECs) origin. We analyzed methylation acetylation status histones (Western...
We have determined the effect of glyphosate on selected epigenetic parameters and major cell cycle drivers in human peripheral blood mononuclear cells (PBMCs). The were incubated with at 0.5, 10 100 μM. analysis included: global DNA methylation, methylation promoter regions tumor suppressor genes (P16, P21, TP53) proto-oncogenes (BCL2, CCND1) by Real-Time PCR expression profile indicated PCR. obtained results revealed significant reduction level PBMCs exposed to glyphosate. Tested compound...
While the total antioxidant capacity (TAC) of blood plasma is mainly accounted for by urate, TAC cell interior can be expected to depend more on other antioxidants, especially glutathione and protein -SH groups. We studied homogenates several lines cultured cells subjected action thiol-modifying agents. Comparison changes level thiols (determined with a biradical spin label) demonstrates that alterations in cellular thiol content main determinant homogenates. These results show estimation...
Abstract Proliferation of human umbilical vein endothelial cells in vitro was inhibited by high concentrations oxidants and nitric oxide donors but stimulated low (micromolar or submicromolar) hydrogen peroxide, menadione, tert ‐butyl hydroperoxide, AAPH, nitroglycerin, SIN‐1 sodium nitroprusside. The stimulation seems to be dependent upon generation secondary reactive oxygen species as inferred from attenuation cell proliferation superoxide dismutase catalase. These results point another...