A5002213243- Acute Lymphoblastic Leukemia research
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Cerebrovascular and Carotid Artery Diseases
- Bioenergy crop production and management
- Peripheral Artery Disease Management
- Advanced biosensing and bioanalysis techniques
- Acute Myeloid Leukemia Research
- Renal and Vascular Pathologies
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Ecology and Vegetation Dynamics Studies
- Forest Ecology and Biodiversity Studies
- CRISPR and Genetic Engineering
- Plant Disease Management Techniques
- Monoclonal and Polyclonal Antibodies Research
- Morphological variations and asymmetry
- Plant and animal studies
- Mycobacterium research and diagnosis
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- Renal Diseases and Glomerulopathies
Masaryk University
2018-2022
University Hospital Brno
2018-2022
Université du Québec en Abitibi-Témiscamingue
2012-2013
Université du Québec
2009
Charles University
2003
Mayo Clinic
2003
Trembling aspen ( Populus tremuloides ) is a clonal tree species, which regenerates mostly through root suckering. In spite of vegetative propagation, maintains high levels diversity. We hypothesized that the maintenance diversity in this species can be facilitated by integrating different clones natural grafts into aspen's communal system. To verify hypothesis, we analyzed systems three pure stands where had been delineated with help molecular markers. Grafting between roots was frequent...
B-cell neoplasms represent a clinically heterogeneous group of hematologic malignancies with considerably diverse genomic architecture recently endorsed by next-generation sequencing (NGS) studies. Because multiple genetic defects have potential or confirmed clinical impact, tendency toward more comprehensive testing diagnostic, prognostic, and predictive markers is desired. This study introduces the design, validation, implementation an integrative, custom-designed, capture-based NGS panel...
Trembling aspen (Populus tremuloides Michx.) as a clonal tree species possesses complex root system through which trees of the same or different clones are connected. Root connections have been studied with respect to resource sharing, but nature, quantities extent what is shared between relatively unknown. In this study, we posed hypothesis that systemic defense induction signals could also spread these networks and trigger defenses in neighboring ramets before arrival pests. Temporal...
Summary Rearrangements of T‐ and B‐cell receptor (TCR BCR) genes are useful markers for clonality assessment as well minimal residual disease (MRD) monitoring during the treatment haematological malignancies. Currently, rearrangements three out four TCR all BCR loci used this purpose. The fourth gene, TRA , has not been so far due to lack a method its rearrangement detection in genomic DNA. Here we propose first high‐throughput sequencing based identification clonal gene at DNA level. is on...
Introduction BCR-ABL1-like acute lymphoblastic leukemia (ALL) is a high-risk disease with complex genomic background. Though extensively studied, data on the frequency and mutual associations of present mutations are still incomplete in adult patients. This retrospective study aims to map landscape B-other ALL cohort patients focus subtype.Methods We analyzed bone marrow peripheral blood samples treated consecutively at three major Czech teaching hospitals. Samples were by cytogenetic...
Retroelements (RE) have been proposed as important players in cancerogenesis. Different cancer types are characterized by a different level of tumor-specific RE insertions. In previous studies, small cohorts hematological malignancies, such acute myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia low insertional activity. Acute lymphoblastic (ALL) adults childhood leukemias not studied this context. We performed search for new insertions (Alu L1) 44 ALL, 14 adult ALL...
Abstract Near‐haploid acute lymphoblastic leukemia is rare subgroup of the disease, which very important due to poor prognosis and resistance treatment including novel monoclonal antibodies CAR‐T therapy.
Summary The aim of the present study was to evaluate significance low‐level minimal/measurable residual disease (MRD) during early consolidation treatment in adult BCR‐ABL1 –negative acute lymphoblastic leukaemia. MRD load monitored by immunoglobulin/T‐cell receptor rearrangements and assessed as negative [complete response (CMR)], positive non‐quantifiable (MRDnq) quantifiable (MRDq). MRDnq before first second blocks had a comparable effect on survival MRDq. 5‐year overall for CMR, MRDq at...
Background: Amplicon‐based next‐generation sequencing (NGS) is increasingly used for identification of clonal immunoglobulin (IG) and T‐cell receptor (TR) gene rearrangements as markers minimal residual disease (MRD) monitoring in acute lymphoblastic leukaemia (ALL). A standardized assay integrating amplicon library preparation well bioinformatic data analysis marker has been established validated a multicentre study by the EuroClonality‐NGS working group, including laboratories from EuroMRD...