A5053637987- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Protein Structure and Dynamics
- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- Polar Research and Ecology
- Electrochemical sensors and biosensors
- thermodynamics and calorimetric analyses
- Microbial Metabolites in Food Biotechnology
- Algal biology and biofuel production
- Polysaccharides and Plant Cell Walls
- Porphyrin Metabolism and Disorders
- Mitochondrial Function and Pathology
- Studies on Chitinases and Chitosanases
- Microbial Metabolism and Applications
- Proteins in Food Systems
- Pancreatic function and diabetes
- Hemoglobin structure and function
- Natural Antidiabetic Agents Studies
- Enzyme-mediated dye degradation
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- biodegradable polymer synthesis and properties
UNSW Sydney
2009-2024
King Fahd University of Petroleum and Minerals
2014-2019
Korean Association Of Science and Technology Studies
2008
King Abdulaziz City for Science and Technology
2000
National Institute for Biotechnology and Genetic Engineering
1994-2000
Imperial College London
1993
Lignocellulose biomass derived from plant cell walls is a rich source of biopolymers, chemicals, and sugars, besides being sustainable alternative to petrochemicals. A natural armor protecting living protoplasts, the wall currently target intense study because its crucial importance in development, morphogenesis, resistance (a)biotic stresses. Beyond intrinsic relevance related overall physiology, constitute an exquisite example composite material that constant inspiration for biotechnology,...
Calcium (Ca2+) has an important structural role in guaranteeing the integrity of outer lipopolysaccharide layer and cell walls bacterial cells. Extracellular DNA (eDNA) being part slimy matrix produced by bacteria promotes biofilm formation through enhanced matrix. Here, concurrent Ca2+ eDNA mediating aggregation was studied for first time using a variety strains thermodynamics to binding. It found that concentrations under both planktonic growth conditions were different among strains....
Psychrophilic (cold-adapted) microorganisms make a major contribution to Earth's biomass and perform critical roles in global biogeochemical cycles. The vast extent environmental diversity of cold biosphere has selected for equally diverse microbial assemblages that can include archaea, bacteria, eucarya, viruses. Underpinning the important ecological psychrophiles are exquisite mechanisms physiological adaptation. Evolution also cold-active traits at level molecular adaptation, enzymes from...
Abstract Carbon nanosheets are two-dimensional nanostructured materials that have applications as energy storage devices, electrochemical sensors, sample supports, filtration membranes, thanks to their high porosity and surface area. Here, for the first time, carbon been prepared from stems leaves of a nettle fibre clone, by using cheap straight-forward procedure can be easily scaled up. The nanomaterial shows interesting physical parameters, namely interconnectivity pores, graphitization,...
Abstract The cold‐adapted α‐amylase from Pseudoalteromonas haloplanktis (AHA) is a multidomain enzyme capable of reversible unfolding. Cold‐adapted proteins, including AHA, have been predicted to be structurally flexible and conformationally unstable as consequence high lysine‐to‐arginine ratio. In order examine the role low arginine content in structural flexibility amino groups lysine were guanidinated form homo‐arginine (hR), structure–function–stability properties modified analyzed by...
ABSTRACT Archaea are abundant and drive critical microbial processes in the Earth's cold biosphere. Despite this, not enough is known about molecular mechanisms of adaptation no biochemical studies have been performed on stenopsychrophilic archaea (e.g., Methanogenium frigidum ). This study examined structural functional properties shock proteins (Csps) from archaea, including analysis Csp M. frigidum. csp genes present most bacteria some eucarya but absent archaeal genome sequences,...
We report the first characterization of an L-arginine:glycine amidinotransferase from a prokaryote. The enzyme, CyrA, is involved in pathway for biosynthesis polyketide-derived hepatotoxin cylindrospermopsin Cylindrospermopsis raciborskii AWT205. CyrA phylogenetically distinct other amidinotransferases, and structural alignment shows differences between active site residues well-characterized human (AGAT). Overexpression recombinant Escherichia coli enabled biochemical we confirmed predicted...
The fasciclin-like arabinogalactan proteins (FLAs) belong to the protein (AGP) superfamily and are known play different physiological roles in plants. This class of was shown participate plant growth, development, defense against abiotic stresses and, notably, cell wall biosynthesis. Although some studies available on characterization FLA genes from species, both woody herbaceous, no detailed information is family textile hemp (Cannabis sativa L.), an economically important fibre crop. By...
ABSTRACT The cold-adapted α-amylase from Pseudoalteromonas haloplanktis unfolds reversibly and cooperatively according to a two-state mechanism at 30°C sequentially with two transitions temperatures below 12°C. To examine the role of four disulfide bridges in activity conformational stability enzyme, eight cysteine residues were reduced β-mercaptoethanol or chemically modified using iodoacetamide iodoacetic acid. Matrix-assisted laser desorption-time flight mass spectrometry analysis...
Abstract The alkaline protease, savinase was chemically modified to enhance the productivity of enzyme at low temperatures on a complex polymeric protein (azocasein) substrate. At 5 and 15°C, with ficol or dextran hydrolyzed fivefold more azocasein than unmodified savinase. Kinetic studies showed that catalytic improvements are associated changes in uncompetitive substrate inhibition K i values savinases sixfold higher Modeling small‐angle scattering data indicates two molecules bind...