A5024260876- Viral Infections and Immunology Research
- S100 Proteins and Annexins
- interferon and immune responses
- Polydiacetylene-based materials and applications
- Cardiac Fibrosis and Remodeling
- Acute Myocardial Infarction Research
- Organometallic Complex Synthesis and Catalysis
- Photorefractive and Nonlinear Optics
- Tissue Engineering and Regenerative Medicine
- Ion channel regulation and function
- Asymmetric Synthesis and Catalysis
- Hormonal Regulation and Hypertension
- Supramolecular Self-Assembly in Materials
- Cyclization and Aryne Chemistry
- Advanced Polymer Synthesis and Characterization
- Metal complexes synthesis and properties
- Organ Transplantation Techniques and Outcomes
- Metal-Catalyzed Oxygenation Mechanisms
- Advanced Drug Delivery Systems
- Fern and Epiphyte Biology
- Oxidative Organic Chemistry Reactions
- Cardiac Structural Anomalies and Repair
- Adenosine and Purinergic Signaling
- Phenothiazines and Benzothiazines Synthesis and Activities
- Peptidase Inhibition and Analysis
German Centre for Cardiovascular Research
2017-2020
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020
Berlin-Brandenburger Centrum für Regenerative Therapien
2016-2018
Charité - Universitätsmedizin Berlin
2013-2018
Barts Health NHS Trust
2017
Institut Kardiale Diagnostik und Therapie
2017
Humboldt-Universität zu Berlin
2017
Freie Universität Berlin
2017
Deutsches Herzzentrum der Charité
2017
Heidelberg University
2017
Background: The alarmins S100A8 and S100A9 are damage-associated molecular patterns, which play a pivotal role in cardiovascular diseases, inflammation, viral infections. We aimed to investigate their Coxsackievirus B3 (CVB3)–induced myocarditis. Methods Results: mRNA expression was 13.0-fold ( P =0.012) 5.1-fold =0.038) higher endomyocardial biopsies from patients with CVB3-positive myocarditis compared controls, respectively. Elimination of CVB3 led downregulation these alarmins....
The cytoplasmatic pattern recognition receptor, NOD2 (nucleotide-binding oligomerization domain 2), belongs to the innate immune system and is among others responsible for of single-stranded RNA. With Coxsackievirus B3 (CVB3) being a RNA virus, recent evidence that target, NLRP3 (NOD-like receptor family, pyrin containing 3) importance in pathogenesis CVB3-induced myocarditis, we aimed unravel role myocarditis.Endomyocardial biopsy mRNA expression was higher CVB3-positive patients compared...
Inflammation in myocarditis induces cardiac injury and triggers disease progression to heart failure. NLRP3 inflammasome activation is a newly identified amplifying step the pathogenesis of myocarditis. We previously have demonstrated that mesenchymal stromal cells (MSC) are cardioprotective Coxsackievirus B3 (CVB3)-induced In this study, MSC markedly inhibited left ventricular (LV) NOD2, NLRP3, ASC, caspase-1, IL-1β, IL-18 mRNA expression CVB3-infected mice. ASC protein expression,...
Regulatory T (Treg) cells offer new therapeutic options for controlling undesired systemic and local immune responses. The aim of the current study was to determine impact Treg administration on cardiac inflammation remodeling in coxsackievirus B3 (CVB3) -induced myocarditis. Therefore, syngeneic were applied intravenously CVB3-infected mice 3 d after infection. Compared with CVB3 + PBS mice, exhibited lower left ventricle (LV) chemokine expression, accompanied by reduced presence...
Abstract Aims The alarmin S100A8/S100A9 (S100A8/A9) is released by activated monocytes/macrophages and neutrophils in the setting lymphocytic myocarditis (MC). We recently demonstrated its therapeutic potential experimental acute MC. Now, we investigated diagnostic relevance of S100A8/A9 serum levels patients with suspected chronic MC heart failure without cardiac inflammation. Methods Results Serum were analysed a recent onset [≤ 30 days, n = 32; ejection fraction (EF): 45.4 ± 12.9%],...
Studies on inflammatory disorders elucidated the pivotal role of CX3CL1/CX3CR1 axis with respect to pathophysiology and diseases progression. Coxsackievirus B3 (CVB3)-induced myocarditis is associated severe cardiac inflammation, which may progress heart failure. We therefore investigated influence CX3CR1 ablation in model acute myocarditis, was induced by inoculation 5x105 plaque forming units CVB3 (Nancy strain) either CX3CR1-/- or C57BL6/j (WT) mice. Seven days after infection, myocardial...
Parathyroid hormone (PTH) is an effective bone anabolic agent. However, only when administered by daily sc injections exposure of short duration achieved, a prerequisite for response. Instead applying exogenous PTH, mobilization endogenous stores the can be envisaged. The secretion PTH stored in parathyroid glands mediated calcium sensing receptor (CaSR) GPCR localized at cell surface. Antagonists CaSR (calcilytics) mimic state hypocalcaemia and stimulate release to bloodstream. Screening...
Abstract Left ventricular (LV) diastolic dysfunction is among others attributed to cardiomyocyte stiffness. Mesenchymal stromal cells (MSC) have cardiac-protective properties. We explored whether intravenous (i.v.) application of PLacenta-eXpanded (PLX) MSC-like improves LV relaxation in streptozotocin (STZ)-induced diabetic mice and investigated underlying mechanisms. Diabetes mellitus was induced by STZ (50 mg/kg body weight) during five subsequent days. One week after the first injection,...
Abstract Cardiac-derived adherent proliferating cells (CardAPs) are derived from human endomyocardial biopsy specimens; they share several properties with mesenchymal stromal cells. The aims of this study were to evaluate whether intramyocardial injection CardAPs modulates cardiac fibrosis and hypertrophy in a mouse model angiotensin II (Ang II)-induced systolic heart failure analyze underlying mechanisms. Intramyocardial application 200,000 improved left ventricular function. This was...
RR01, a new highly lipophilic drug showing extremely low water solubility and poor oral bioavailability, has been incorporated into pH-dependent dissolving particles made of poly(methacrylic acid-co-ethylacrylate) copolymer. The physicochemical properties the were determined using laser-light-scattering techniques, scanning electron microscopy, high-performance liquid chromatography, x-ray powder diffraction. Suspension free in solution hydroxypropylcellulose (reference formulation) aqueous...
Abstract Aims Left ventricular (LV) dysfunction in viral myocarditis is attributed to myocardial inflammation and fibrosis, inducing acute long‐time cardiac damage. Interventions are not established. On the basis of link between inflammation, aldosterone, extracellular matrix regulation, we aimed investigate effect an early intervention with mineralocorticoid receptor antagonist (MRA) eplerenone on remodelling a murine model persistent coxsackievirus B3 (CVB3)‐induced myocarditis. Methods...
Abstract We report on absorption and fluorescence of single crystals the unsymmetrically substituted diacetylene TS/FBS during UV-photopolymerization thermal polymerization. All observed reaction intermediates have been identified as diradicals, carbenes stable oligomers. compare these results with well-known TS6. The quantum yield initiation step well activation energy following dark determined. Well-separated yellow zones are characteristic for crystals: An additional blue-shifted polymer...
Abstract The synthesis of two new asymmetrical diacetylene (DA) derivatives is described: 1‐(4‐fluorobenzenesulfonyloxy)‐6‐(4‐trifluoromethylbenzenesulfonyloxy)‐2,4‐hexadiyne (FBS/TFMBS) and 1‐(2,4‐dinitrophenoxy)‐6‐(4‐methyl‐2‐nitrophenoxy)‐2,4‐hexadiyne (DNP/MNP). Results are presented on crystal growth, structure analysis dielectric properties. Both DAs polymerize thermally in the solid state have polar (“ferroelectric”) individual chains. different chains unit cell arranged...
Abstract The thermal solid state polymerization was analysed for topospecifically deuterated derivatives of 2,4‐hexadiynylene di‐ p ‐toluenesulfonate (PTS). Deuteration the methylene groups close to diacetylene‐monomer triple bonds influences drastically length induction period and reaction‐rate constants in autocatalytic period. These peculiarities were investigated by combination dielectric permittivity measurements with differential scanning calorimetry gravimetrical analyses polymerization.